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Method for producing single-cell protein by mixing trichoderma reesei and saccharomyces cerevisiae to ferment white spirit vinasse

A technology of single-cell protein and Saccharomyces cerevisiae, which is applied in the field of mixed fermentation of distiller's grains produced by Trichoderma reesei and Saccharomyces cerevisiae, can solve the problems of feed palatability inspection, poor repeatability, complex control of four bacteria, etc., and achieve good results Industrial application potential, high conversion rate, and obvious synergistic effect

Active Publication Date: 2021-08-31
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mixed fermentation of Geotrichum candidum, Aspergillus oryzae, Trichoderma viride and Bacillus subtilis has high utilization efficiency and high protein yield, but the regulation of the four bacteria is complicated and the repeatability is poor. The palatability of the four bacteria needs to be tested when used as feed

Method used

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  • Method for producing single-cell protein by mixing trichoderma reesei and saccharomyces cerevisiae to ferment white spirit vinasse
  • Method for producing single-cell protein by mixing trichoderma reesei and saccharomyces cerevisiae to ferment white spirit vinasse
  • Method for producing single-cell protein by mixing trichoderma reesei and saccharomyces cerevisiae to ferment white spirit vinasse

Examples

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Embodiment Construction

[0026] The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples were purchased from commercial sources unless otherwise specified.

[0027] 1 Experimental materials and methods

[0028] 1.1 Materials

[0029] Distiller's grains: from Xifeng Distillery; strains: Trichoderma reesei Rut-C30, Saccharomyces cerevisiae INVSc1, commercially available strains; reagents: all commercially available analytically pure.

[0030] 1.2 Medium

[0031] (1) PDA medium

[0032] (2) YPD medium: yeast extract 1%, peptone 2%, glucose 2%

[0033] (3) Seed medium: peptone 0.05g / bottle, ammonium sulfate 0.07g / bottle, potassium dihydrogen phosphate 0.1g / bottle, trace elements 0.05ml / bottle, urea 0.015g / bottle, magnesium sulfate 0.015g / bottle, chlorine Calcium 0.02g / bottle, citric acid 2.5ml / bottle, Tween 80 2 drops / bottle, 0.1g / ml glucose 5ml / bottle, distilled water 42.5ml / bottle

[0034] (4) Fermenta...

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Abstract

The invention discloses a method for producing single-cell protein by mixing trichoderma reesei and saccharomyces cerevisiae to ferment white spirit vinasse, which comprises the following steps: inoculating the trichoderma reesei into a fermentation medium containing the white spirit vinasse for fermentation, and inoculating the saccharomyces cerevisiae into the white spirit vinasse for continuous fermentation after 0-36 hours, the total fermentation time is 5-6 days, the total inoculation amount of the trichoderma reesei and the saccharomyces cerevisiae is 4-10%, and the inoculation biomass ratio of the trichoderma reesei to the saccharomyces cerevisiae is 1: 3-5: 1. A double-fungus microbial colony formed by the trichoderma reesei and the saccharomyces cerevisiae is obvious in synergistic effect, free of obvious antagonism and simple to regulate and control, 26.7 g of SCP can be produced from 33.3 g of vinasse after condition optimization, and the conversion rate is high.

Description

technical field [0001] The invention belongs to the field of fermentation, and in particular relates to a method for producing single-cell protein by mixed fermentation of distiller's grains by Trichoderma reesei and Saccharomyces cerevisiae. Background technique [0002] Distiller's grains are the waste residues left after brewing rice, wheat, sorghum, etc. It comes from my country's unique liquor brewing technology and contains a certain proportion of nutrients, especially crude protein, lysine, methionine and tryptophan. etc. and a variety of trace elements and vitamins, have a certain reuse value, and can turn waste into treasure and reuse it as animal feed so as to save the concentrate for feeding animals and reduce the investment in breeding. [0003] There have been many related reports on patents and papers on single-cell protein (SCP) produced by mixed fermentation of various distiller's grains by microbial flora, but the various technologies used are different, each...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/16C12R1/885C12R1/865
CPCC12N1/14C12N1/16Y02P60/87
Inventor 方浩鲁辰雨邓云涛
Owner NORTHWEST A & F UNIV
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