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Preparation method of hierarchical porous metal organic framework chiral sensing probe, probe obtained by preparation method and application of probe

A metal-organic framework and hierarchical porous technology, which is applied in the fields of enzyme immobilization and analytical chemistry, can solve problems such as interference, and achieve the effects of improving stability, avoiding aggregation quenching, and high porosity

Active Publication Date: 2021-08-27
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, fluorescent probes rarely allow simultaneous enantioselective and chemoselective recognition of specific amino acids, and may be interfered by other similar biochemical molecules

Method used

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  • Preparation method of hierarchical porous metal organic framework chiral sensing probe, probe obtained by preparation method and application of probe
  • Preparation method of hierarchical porous metal organic framework chiral sensing probe, probe obtained by preparation method and application of probe
  • Preparation method of hierarchical porous metal organic framework chiral sensing probe, probe obtained by preparation method and application of probe

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Experimental program
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Effect test

Embodiment 1

[0047] 1.1 Synthesis of fluorescent molecules (PF)

[0048] 1,2,4-phenyltrhane and mass of 5.19 g of 3-15 g of 3-bromophenol and a mass of 15 ml were added to the tri-neck flask. After 72 hours at 135 ° C, the mixture was added to 120 ml of ice ion water, and the green solid was stirred, then the obtained solid was collected by vacuum filtration. The mixed reagent (V / V = ​​1: 3) was washed several times with pyridine and acetic anhydride, and a mixed reagent (V / V = ​​2: 1) of acetic anhydride and pyridine was recrystallized 3 times at 100 ° C, and then the concentration was used. 1 mol L -1 The hydrochloric acid was washed several times, and finally, the white solid powder was obtained at 85 ° C for 12 h.

[0049] PD (DPPF) CL of a mass of 400 mg of boron and a mass of 200 mg of a mass of 400 mg of a boron, a mass of 98.9 mg. 2 And the mass of 510 mg of anhydrous acetate is loaded into the dry three-neck flask and the system is vacuum and then reflects nitrogen. This process ...

Embodiment 2

[0073] Referring to Example 1 1.2-1.4 Changing the fixed amino acid oxidase, fixed L-glutamate oxidase (L-GOX) into a hierarchical mesh metal organic skeleton microsphere, resulting in L-GOX & PF @ HPUIO-66 probe material.

[0074] See Example 1 1.5 Alter the L / D-amino acid as L / D-glutamic acid. Figure 24 A It can be seen that L-GOX & PF @ HPUIO-66 explores that L-glutamic acid has chiral sensor.

[0075] See Example 1 1.6 Changing the L-AAO & PF @ HPUIO-66 probe material is L-GOX & PF @ HPUIO-66 probe material; changing the L-amino acid is L-glutamic acid. Figure 24 B and Figure 24 C It can be seen that the L-GOX & PF @ HPUIO-66 probe can be linearized in L-glutamic acid within 0 to 100 μm.

[0076] 19 μl of the amino acid solution (100 μm) with a chiral enantiomer (100 μm) was added to 1.9 mL of L-GOX & PF @ HPUIO-66 probe solution (100 mg L -1 )middle. The fluorescence intensity of the reaction mixture at λ = 520 nm was measured at 37 ° C to the reaction equilibrium. Figure...

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Abstract

The invention relates to a preparation method of a hierarchical porous metal organic framework chiral sensing probe, which comprises the following steps of providing a hierarchical porous metal organic framework microsphere with a regular pore structure, fixing amino acid oxidase on the hierarchical porous metal organic framework microspheres to obtain hierarchical porous metal organic framework microspheres fixed with enzymes, and loading the amino acid oxidase in pore channels of the hierarchical porous metal organic framework microspheres through adsorption, fixing fluorescent molecules on the hierarchical porous metal organic framework microspheres fixed with enzyme to obtain the hierarchical porous metal organic framework chiral sensing probe, and loading the fluorescent molecules in pore channels of the hierarchical porous metal organic framework microspheres through adsorption. The invention also provides the hierarchical porous metal organic framework chiral sensing probe obtained by the preparation method and application thereof. By fixing different amino acid oxidase as a chiral recognition center, the amino acid can be recognized and the content of the amino acid can be analyzed in a high-sensitivity and high-specificity manner.

Description

Technical field [0001] The present invention relates to enzyme immobilization and analysis chemistry, and more particularly to a method of preparing a hierarchical porous metal organic frame-leaf-sensitive probe and a probe obtained thereby and its application thereof. Background technique [0002] Amino acids are one of the most important and most important chiral compounds in nature (Angew.Chem.It.ed.2017, 56, 7276-7281). L-amino acids and D-amino acids are not mutually exclusive, and is usually coexisting in the form of a non-racemic mixture. In mammals and humans, amino acids are typically present in a free form or in the form of a protein. In general, most of the amino acids in the organism and nature present in the form of L form, and a large amount of D form is generally negative symptoms, aging or diseases (ACS Appl.mater.Iterfaces 2017, 9, 201091-20999). The production of amino acids, and the felt is important in life science (Amino Acids 2012, 42, 1553-1582). Amino acid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/01
CPCG01N21/6486G01N21/01
Inventor 顾金楼杨健
Owner EAST CHINA UNIV OF SCI & TECH
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