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Bacillus cereus strain YN917 and application thereof

A technology of Bacillus cereus and bacterial strains, applied in the field of biological control, can solve the problems of unstable disease control effect, poor colonization ability of antagonistic microorganisms, and reduced control effect of antagonistic microorganisms, so as to achieve good application prospects, avoid adverse effects, and dry re-enhancing effect

Active Publication Date: 2021-08-20
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the field of biological control, there are often problems such as poor colonization ability of antagonistic microorganisms under natural conditions in the field and unstable effect on disease control.
A large number of studies have also shown that the antagonistic microorganisms screened indoors have a strong inhibitory effect on pathogenic bacteria in the greenhouse, but the effect in field control is not satisfactory. Colonize the root circle of plants, on the other hand, interact with the complex microbial species and antagonistic strains in the field soil, resulting in a decrease in the control effect of antagonistic microorganisms. Therefore, it is necessary to select and screen biological strains with strong field adaptability or high-efficiency metabolites Applying to field disease control is an important way to solve the above problems

Method used

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  • Bacillus cereus strain YN917 and application thereof
  • Bacillus cereus strain YN917 and application thereof
  • Bacillus cereus strain YN917 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1 The isolation and acquisition of Bacillus cereus bacterial strain YN917

[0030] Healthy rice plants were selected from diseased clusters of the rice variety Xiangzaoxian 24 infected with rice blast in Paixingshang Village, Daligang Town, Taojiang County, Hunan Province (N28°31′49.02″, E111°54′24.86″), and the rice strains were used with the blast fungus An antagonistic bacteria strain was screened out by plate confrontation method, and the inhibition rate of the strain against Magnaporthe oryzae was 72.63%±1.30%.

[0031] Cultivate the bacterial strain on LB solid medium (tryptone 10g / L, yeast extract 5g / L, NaCl 5g / L, agar 20g / L, pH7.0, distilled water preparation) plate, observe its colony morphology, and The scanning electron microscope of its bacterium observes, and its bacterium colony form is as follows figure 1 As shown, the colonies are large, soft, rough, irregular in shape, and slightly shiny white colonies (candle-like color). After long-term culti...

Embodiment 2

[0035] Example 2 Bacillus cereus strain YN917 disease prevention and growth promotion effect

[0036] After the bacterial strain YN917 was activated on the LB solid medium plate, two bacterium cakes with a diameter of 5 mm were obtained with a punch and inserted into 150 mL of LB liquid medium (tryptone 10 g / L, yeast extract 5 g / L, NaCl 5 g / L L, pH 7.0, distilled water) in a 250mL Erlenmeyer flask, 28°C, 180r / min conditions in a shaker for 24h, to obtain the metabolites of Bacillus cereus strain YN917.

[0037] Add 1mL of Bacillus cereus strain YN917 and 1mL of rice blast fungus conidia liquid into a sterilized centrifuge tube and mix well, replace the culture medium of strain YN917 with sterile water as a control, cultivate in the dark at 28°C, and observe under the microscope every 2 hours For spore germination, the experiment was repeated 3 times. The results showed that strain YN917 had inhibitory effect on the germination of Magnaporthe grisea spores.

[0038] Taking Li...

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Abstract

The invention provides a bacillus cereus strain, which is named as YN917 and is preserved in the China Center for Type Culture Collection on October 29, 2020, and the preservation number is CCTCC NO: M2020655. The strain can be used for treating and preventing rice blast, has a good control effect on the rice blast, has protection and treatment effects, can promote rice growth, can be processed and developed as a potential biological control agent and a biological inoculant for rice cultivation, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of biological control, and more specifically relates to a Bacillus cereus strain YN917 and its application in treating and preventing rice blast fungus and its application in rice growth promotion. Background technique [0002] Rice (Oryza sativa) is one of the most important food crops. It is widely planted in more than 120 countries and regions, accounting for 1 / 4 of the world's total arable land. More than half of the world's population feeds on rice. Rice blast (the pathogen is Magnaporthe oryzae, and its asexual generation is Pyiicularia oryzae) is one of the most serious fungal diseases of rice. The disease can be divided into seedling blast, leaf blast, node blast, and panicle blast according to its location and onset period. , grain blast, branch blast, etc. The disease has become a serious obstacle to the sustained high and stable yield of rice. At present, the main measures to prevent and control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C05F11/08C05G3/00C05G3/60C12R1/085
CPCC12N1/20A01N63/22C05F11/08C05G3/00C05G3/60
Inventor 刘二明周瑚邹秋霞任佐华
Owner HUNAN AGRICULTURAL UNIV
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