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Vascularized bone bionic multifunctional tissue engineering scaffold with anti-inflammatory effect and preparation method thereof

A technology of tissue engineering scaffold and anti-inflammatory effect, applied in tissue regeneration, pharmaceutical formula, coating, etc., can solve the problems of prolonging the healing time of bone defects, and achieve the effect of promoting vascularization, anti-inflammatory preparation method, and good comprehensive performance

Active Publication Date: 2021-08-20
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Finally, we considered the issue of bone tissue engineered scaffolds causing foreign body reactions around the implant, triggering inflammation and prolonging the healing time of bone defects

Method used

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  • Vascularized bone bionic multifunctional tissue engineering scaffold with anti-inflammatory effect and preparation method thereof
  • Vascularized bone bionic multifunctional tissue engineering scaffold with anti-inflammatory effect and preparation method thereof
  • Vascularized bone bionic multifunctional tissue engineering scaffold with anti-inflammatory effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) The cortical and cancellous bone structure that simulates natural bone is modeled by AutoCAD, 3DSMAX and other modeling software to obtain a three-dimensional model.

[0056] (2) Using FDM technology, biomimetic PLA scaffolds with gradient pore sizes of 500 μm and 900 μm were prepared by 3D printers. (3) At room temperature, the PLA scaffolds were soaked in ammonia solution with a pH value of 12 and stirred at a speed of 180 rpm for 4 h, and then the alkali-treated PLA scaffolds were soaked in a concentration of 1% ( W / V ) in the HA ethanol solution at 180 rpm for 1.5 h, then placed the PLA scaffold in absolute ethanol and sonicated for 3 min to remove excess HA on the surface of the scaffold, and finally air-dried at room temperature.

[0057] (4) Weigh 10 g of gelatin and dissolve it in 100 mL of PBS solution, and stir for 1 h in a warm bath at 45 °C until completely dissolved. Slowly add 8 mL of MA into the gelatin solution dropwise, and after stirring for 2 h, ...

Embodiment 2

[0063](1) The cortical and cancellous bone structure that simulates natural bone is modeled by AutoCAD, 3DSMAX and other modeling software to obtain a three-dimensional model.

[0064] (2) Using FDM technology, biomimetic PLA scaffolds with gradient pore sizes of 400 μm and 800 μm were prepared by 3D printers.

[0065] (3) At room temperature, soak the PLA scaffold in an ammonia solution with a pH value of 12 and stir it at a speed of 175 rpm for 4 hours, and then soak the alkali-treated PLA scaffold in a concentration of 1% ( W / V ) in the HA ethanol solution at 180 rpm for 2 h, then placed the PLA scaffold in absolute ethanol and sonicated for 3 min to remove excess HA on the surface of the scaffold, and finally air-dried at room temperature.

[0066] (4) Weigh 10 g of gelatin and dissolve it in 100 mL of PBS solution, and stir in a warm bath at 50 °C for 1 h until completely dissolved. 8 mL of MA was slowly added dropwise to the gelatin solution, and after stirring for 1.5 ...

Embodiment 3

[0072] (1) The cortical and cancellous bone structure that simulates natural bone is modeled by AutoCAD, 3DSMAX and other modeling software to obtain a three-dimensional model.

[0073] (2) Using FDM technology, biomimetic PLA scaffolds with gradient pore sizes of 450 μm and 850 μm were prepared by 3D printers.

[0074] (3) At room temperature, soak the PLA scaffold in an ammonia solution with a pH value of 12 and stir at a speed of 180 rpm for 4 hours, and then soak the PLA scaffold after alkali treatment in a concentration of 1% ( W / V ) in the HA ethanol solution at 180 rpm for 1.5 h, then placed the PLA scaffold in absolute ethanol and ultrasonicated for 3 min to remove excess HA on the surface of the scaffold, and finally air-dried at room temperature.

[0075] (4) Weigh 10 g of gelatin and dissolve it in 100 mL of PBS solution, and stir for 1 h in a warm bath at 45 °C until completely dissolved. Slowly add 8 mL of MA into the gelatin solution dropwise, and after stirring...

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Abstract

The invention discloses a vascularized bone bionic multifunctional tissue engineering scaffold with an anti-inflammatory effect and a preparation method thereof. The vascularized bone bionic multifunctional tissue engineering scaffold mainly comprises four components, namely an HA(at)PLA scaffold, GelMA, DFO-NPs and MnCO. An HA(at)PLA scaffold is prepared through a 3D printing technology and a surface coating manner, a mixed solution of GelMA prepolymer, MnCO and DFO-NPs is injected into the scaffold, UV photocrosslinking is performed to obtain the bionic multifunctional composite scaffold, and according to the bionic multifunctional composite scaffold prepared by combining the components, due to the release of MnCO and DFO-NPs, the bone repair can be well promoted by diminishing inflammation and promoting the formation of blood vessels, and a theoretical basis and technical support are provided for an existing clinical treatment scheme for large-segment bone defect repair.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a vascularized bone bionic multifunctional tissue engineering scaffold with anti-inflammatory effect and a preparation method thereof. Background technique [0002] Bone has a strong ability to repair itself, but it cannot completely repair large segmental bone defects. There are tens of millions of patients with bone defects in the world every year, and large bone defects caused by tumors and trauma are the main reasons for clinical bone repair and transplantation. The best material for bone grafting is autologous bone, but the source of autologous bone is limited, and it will increase the suffering of patients, making it difficult to meet the actual needs. The emergence of bone tissue engineering scaffolds provides more diverse repair materials for this type of surgery. In recent years, bone tissue engineering has frequently achieved new results, and the prep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/32A61L27/02A61L27/18A61L27/22A61L27/52A61L27/54A61L27/56A61L27/58
CPCA61L27/18A61L27/32A61L27/222A61L27/025A61L27/56A61L27/52A61L27/54A61L27/58A61L2300/102A61L2300/216A61L2300/41A61L2300/412A61L2400/12A61L2430/02C08L67/04C08L89/00
Inventor 张进杨黄浩童冬梅阮任杰陈琳臧浩苏伟玲邹蕴
Owner FUZHOU UNIV
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