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Thin-layer chromatography identification method of rhizoma corydalis stomach'an capsule

A technology of thin-layer chromatography and chromatography, applied in the field of drug analysis, to achieve the effects of clear judgment of results, elimination of interference, and control of drug quality

Active Publication Date: 2021-08-13
段复华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are 9 herbs in the prescription of this product, and the current drug standard only points to the qualitative identification items of 2 herbs, which has a large gap with the actual needs of quality control of traditional Chinese medicine

Method used

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  • Thin-layer chromatography identification method of rhizoma corydalis stomach'an capsule
  • Thin-layer chromatography identification method of rhizoma corydalis stomach'an capsule
  • Thin-layer chromatography identification method of rhizoma corydalis stomach'an capsule

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Experimental program
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Effect test

preparation example Construction

[0062] (1) Preparation of the test solution:

[0063] Take 2 g of Yanhu Wei'an Capsules, add 20 ml of solvent for ultrasonic or reflux treatment for 30 minutes, filter, concentrate the filtrate to 2 ml, filter with a 0.45 μm microporous membrane, and use it as the test solution;

[0064] (2) Thin-layer chromatographic identification of chicken saigenin:

[0065] Take 1.0 mg of gallinaceous savine reference substance, dissolve it in 1 mL of methanol, filter it with a 0.45 μm microporous membrane, and use it as the reference substance solution;

[0066] Draw 2 μL each of the test solution and the reference solution, respectively, and place them on the same thin-layer chromatography plate, use chloroform-methanol-glacial acetic acid-water solution as the developing agent to develop upward, take it out, and dry it in the air; spray the thin-layer chromatography with 10% sulfuric acid ethanol solution, heated at 105°C until the color of the spots is clear; the chromatogram of the ...

Embodiment 1

[0097] Exploration of Qualitative Conditions of Thin Layers

[0098] 1. Choice of extraction solvent

[0099] ①Weigh 500g of each raw medicinal material. Then the 500g raw medicinal material is divided into two parts of 400g and 100g. The 400g part was heated and extracted with 10 times the mass of water, boiled for 30 minutes, then filtered and concentrated to 100ml; the 100g part was ultrasonically extracted by adding 5 times the mass of methanol for 30 minutes, filtered and concentrated to 100ml.

[0100]② Take 8g of Yanhu Wei’an Capsules, 6 parts in total, 3 parts were ultrasonicated with 10 times the mass of 75% ethanol, methanol and water for 30 minutes; the remaining 3 parts were heated in a water bath with 10 times the mass of 75% ethanol, methanol and water respectively Reflux for 30 minutes, and filter to obtain the filtrate.

[0101] ③ According to the mass ratio in the formula of Yanhu Wei'an Capsules, take the original medicinal materials to make capsules, a to...

Embodiment 2

[0116] TLC qualitative identification

[0117] Reference substances: all were provided by Chengdu Aifa Biotechnology Co., Ltd. (Chengdu, China), with a purity greater than 98%.

[0118] TLC plate: silica gel G plate of Qingdao Ocean Chemical Co., Ltd.

[0119] TLC identification: the first 3 points in the thin-layer plate are three batches of capsule test solution, the batch numbers are: 20190701LSH01, 20200701 LSH95, 20201101 LSH31, the fourth point is the reference substance, and the fifth point is the capsule negative solution.

[0120] 1. Method for TLC identification and determination of chicken savine glycosides

[0121] 1.1 Preparation of the test solution

[0122] Make capsules according to the prescription and process, take 2g of capsules, add 20ml of methanol, ultrasonically treat for 30 minutes, filter, concentrate the filtrate to 2ml, filter with a 0.45μm microporous membrane, and use it as the test solution;

[0123] 1.2 Preparation of reference medicinal solu...

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Abstract

The invention belongs to the field of pharmaceutical analysis, and provides a thin-layer chromatography identification method of a rhizoma corydalis stomach'an capsule, the method is characterized in that: paederin, 1, 4-di [4-(glucose oxygen) benzyl]-2-isobutyl malate, dehydrocostus lactone, costunolide, 6-gingerol, oleanolic acid, liquiritin, tetrahydropalmatine and tetrahydropalmatine are added as reference substances, identification objects respectively point to thin-layer chromatography qualitative identification indexes of paederia scandens, rhizoma bletillae, radix aucklandiae, fresh ginger, Chinese dates, liquorice and rhizoma corydalis which are main medicinal materials of a formula of the rhizoma corydalis stomach'an capsule. Compared with the current drug standard of the rhizoma corydalis stomach'an capsule, the method overcomes the technical defect that the current standard of the rhizoma corydalis stomach'an capsule has few control points for identifying the quality of the medicinal materials of the formula, and the provided identification item index is more comprehensive, strong in specificity, simple and rapid to operate, visual and clear in judgment and objective and reliable in conclusion, a technical method is provided for perfecting and improving the medicine quality standard of the rhizoma corydalis stomach'an capsule and better controlling the medicine quality.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a thin-layer chromatographic identification method for Yanhu Wei'an capsules. Background technique [0002] The ethnic medicine "Yanhu Wei'an Capsule" consists of 9 medicinal materials in the prescription: Jishiteng, sea octopus, jujube, amomum, corydalis, woody, baiji, licorice, and ginger. The functions and indications are soothing the liver and stomach. Acid relieves pain. It is used for liver-kidney disharmony syndrome, such as vomiting, acid regurgitation, abdominal distension and pain, lack of appetite, etc. [0003] "Yanhu Wei'an Capsules" drug standard WS-10798 (ZD-0798)-2002 qualitative identification only has 2 TLC identifications with Muxiang medicinal materials and Corydalin as reference substances, and the identification targets point to the prescription medicinal materials wood Xiang and Corydalis, and the remaining seven medicinal materials have...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 段复华杨洪英饶无忌
Owner 段复华
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