Thin-layer chromatography identification method for agastache rugosus Wanying powder

A technology of thin-layer chromatography and Huoxiang, which is applied in the field of thin-layer chromatography identification of Huoxiang Wanying Powder, achieves good separation effect, simplified experimental operation steps, and clear result judgment

Inactive Publication Date: 2021-10-22
段复华
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are 11 medicinal materials in the prescription of this product, and the current drug standards only point to the qualitative identification items of 4 medicinal materials, which has a large gap with the actual needs of quality control of traditional Chinese medicine

Method used

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  • Thin-layer chromatography identification method for agastache rugosus Wanying powder
  • Thin-layer chromatography identification method for agastache rugosus Wanying powder
  • Thin-layer chromatography identification method for agastache rugosus Wanying powder

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Embodiment 1

[0090] Reference substances: all were provided by Chengdu Aifa Biotechnology Co., Ltd. (Chengdu, China), with a purity greater than 98%.

[0091] TLC plate: silica gel G plate of Qingdao Ocean Chemical Co., Ltd.

[0092] TLC identification: the first 3 points in the thin-layer plate are three batches of powder test solution, the batch numbers are: 20180401, 20190301, 20200301, the fourth point is the reference substance, and the fifth point is the powder negative solution

[0093] 1 white pepper

[0094] 1.1 Preparation of the test solution

[0095] Make powder according to the prescription and process, take 5g of powder, add methanol 50ml, ultrasonically treat for 30 minutes, filter, concentrate the filtrate to 5ml, filter with 0.45μm microporous membrane, and use it as the test solution.

[0096] 1.2 Preparation of reference medicinal solution

[0097] Take 1.0 mg of piperine reference substance, dissolve it in 1 mL of methanol, and filter it with a 0.45 μm microporous me...

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Abstract

The invention belongs to the field of ethnic drug analysis, and provides a thin-layer chromatography identification method of agastache rugosus Wanying powder, which is characterized in that piperine, atractydin, oleanolic acid, 6-gingerol, evodiamine and rutaecarpin are added as reference substances; identification objects respectively point to thin-layer chromatography qualitative identification indexes of white pepper, rhizoma atractylodis, jujube, dried ginger and fructus evodiae which serve as main medicinal materials of the formula of the agastache rugosus Wanying powder. Compared with the current drug standard of the agastache rugosus Wanying powder, the method provided by the invention overcomes the technical defect that the current standard of the agastache rugosus Wanying powder has few quality control points for identifying the medicinal materials of the formula, and the provided identification item index is more comprehensive, strong in specificity, simple, convenient and rapid to operate, intuitive and clear in judgment and objective and reliable in conclusion; the invention provides a technical method for perfecting and improving the quality standard of the ethnic medicine 'agastache rugosus Wanying powder' and better controlling the quality of the medicine.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a TLC identification method for Huoxiang Wanying Powder. Background technique [0002] The national medicine "Huoxiang Wanying San" prescription consists of 11 medicinal materials, including fenugreek, magnolia bark, tangerine peel, atractylodes atractylodes, patchouli, jujube, amomum, evodia, clove, dried ginger, and white pepper. The functions and indications are as follows: Relieve exterior and dispel cold, regulate qi and remove dampness, harmonize stomach and relieve pain. It is used for headache, nasal congestion, nausea and vomiting, epigastric pain and other diseases caused by exogenous wind-cold and internal injury damp stagnation. [0003] "Huoxiang Wanying San" drug standard WS-10166 (ZD-0166)-2002-2012Z qualitative identification only has 4 items of TLC identification with Atractylodes herb, hesperidin, magnolol and eugenol as reference substances, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N30/95
CPCG01N30/90G01N30/94G01N30/95
Inventor 段复华杨洪英饶无忌
Owner 段复华
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