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An anomalous Wickhamian yeast strain capable of degrading nitrite

A nitrite and Wickham's technology, applied in the field of bioengineering, can solve the problem of few detailed reports of yeast, and achieve the effects of no pollution to the environment, stable degradation, and improvement of water quality

Active Publication Date: 2022-04-01
山东工匠生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from the existing patents and literature, there are few detailed reports on yeast as a nitrite-removing strain

Method used

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  • An anomalous Wickhamian yeast strain capable of degrading nitrite
  • An anomalous Wickhamian yeast strain capable of degrading nitrite
  • An anomalous Wickhamian yeast strain capable of degrading nitrite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and identification of abnormal Wickham yeast WP-1

[0032] The original water sample was taken from Wanping Scenic Area, Rizhao City, Shandong Province; 12.5mL water sample was enriched in a 250mL sterile Erlenmeyer flask containing nitrite enrichment medium, 72h at 28°C; 12.5mL fresh enrichment medium was added every day Medium.

[0033] After 72 hours of incubation at 28°C, the inoculated strains were diluted 10 times, and 0.5 mL was evenly spread on the surface of the separation medium, and incubated at 28°C for 72-96 hours.

[0034] After the samples were naturally dried, the petri dish was placed upside down and placed in a constant temperature incubator at 30°C for cultivation. According to the morphological characteristics and dominance of the colonies, 8 representative strains were isolated. According to the color and morphological characteristics of the colony, select a representative single colony, and organize the purification medium pla...

Embodiment 2

[0059] Example 2 Safety detection test of abnormal Wickham yeast (WP-1)

[0060] Litopenaeus vannamei (Penaeus vannamei) is one of the important aquatic species, so this strain was selected for biosafety evaluation:

[0061] Healthy shrimp (3±1cm) were selected from a farm in Rizhao City, cultured in artificial seawater prepared with 8‰ sea salt 7 days in advance, and tap water that had been aerated was selected as experimental water. ClO 2 After disinfection, the shrimps were randomly divided into 3 experiments and 3 control groups, each in a 5L artificial seawater pool (Yantai Yi Plastic Packaging Products Co., Ltd.), with 30 shrimps in each group. Centrifuge 100mL bacterial liquid (1.5×10 8 cfu / mL (the optimal inoculum amount obtained through optimization experiments), resuspend 10 mL of sterilized seawater, and wash 2-3 times with the same volume of sterilized seawater. In the experimental group, 1 mL of suspended bacterial cells was added to each water tank, and in the...

Embodiment 3

[0064] The influence of embodiment 3 temperature, pH, carbon source type and C / N on the growth rate of WP-1 bacterial strain

[0065] In a 250mL Erlenmeyer flask, add 1.4×10 8 cfu of WP-1-1 5% (v / v) strain solution and 95% (v / v) basal medium: 0.05g NaNO 2 , 0.5g glucose, 0.05g KH 2 PO 4 , 0.01g MgSO 4 ·7H 2 O, 0.5mL FeSO 4 ·EDTANa 2 (*1000) and sterile water to make a total volume of 100mL.

[0066] Cultivate in a shaker at 180r / min for 48h, repeat 3 times.

[0067] The pH is set to 4, 5, 6, 7, 8, 9; the temperature is set to 15, 20, 25, 30, 35, 40°C;

[0068] The type of carbon source is sucrose, maltose, glucose, glycerol and no carbon source as a control (the addition amount of different carbon sources is 0.5g);

[0069] C / N (glucose / sodium nitrite) is 10:1, 15:1, 20:1, 25:1, 30:1 and 35:1; (the above ratio adjustment is achieved by changing the amount of substances added to the medium)

[0070] Under the settings of different pH, temperature, carbon source and C / ...

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Abstract

The invention belongs to the technical field of bioengineering, and provides a Wickerhamomyces anomalus strain capable of degrading nitrite. The strain is identified as Wickerhamomyces anomalus through 18SrDNA strain identification, sequencing sequence analysis, The strain has been preserved in the China Center for Type Culture Collection (CCTCC) in January 2021, and the preservation number is: CCTCC M 2021030. It has been tested that the strain can degrade nitrite and ammonium stably when used in aquaculture ponds. Nitrogen will not be toxic to beneficial microorganisms in the water body and aquaculture organisms such as fish, shrimp and crab, and can improve the water quality of aquaculture ponds; it can also be used for the removal of nitrite in other aspects.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and provides an abnormal Wickham yeast strain capable of degrading nitrite and an application thereof. Background technique [0002] The accumulation of nitrite is one of the main causes of water pollution in aquaculture and an important environmental factor that induces outbreaks of aquatic animal diseases. In aquaculture, in addition to farmed animals, there are a large number of phytoplankton and algae, and only feeding on these plants is far from meeting the needs of high-density aquaculture animals. Therefore, artificial bait is needed to meet the nutritional needs of farmed animals. However, in order to pursue high profits in a short period of time, many breeding enterprises and farmers add too much artificial bait, combined with the accumulation of excrement and secretions of breeding animals, resulting in serious pollution of breeding water, the most important nitrogen pollutant is ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C02F3/34C12R1/645C02F101/16
CPCC02F3/347C02F2101/166C02F2101/16
Inventor 李震苗译文张力李泽谷国林陈晋波李敬龙
Owner 山东工匠生物科技有限公司
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