Application of Jinfei capsule in preparation of anti-breast cancer drugs

A technology for breast cancer, breast cancer cells, applied in the field of biopharmaceuticals

Pending Publication Date: 2021-08-10
THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant record on the use of Jingan Capsules for the prevention and treatment of breast cancer

Method used

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  • Application of Jinfei capsule in preparation of anti-breast cancer drugs
  • Application of Jinfei capsule in preparation of anti-breast cancer drugs
  • Application of Jinfei capsule in preparation of anti-breast cancer drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Cell Viability Test

[0037] Preparation of sample solution: The contents of Jingan capsules were prepared with physiological saline to prepare sample solutions with concentrations of 0.125mg / ml, 0.25mg / ml, 0.5mg / ml, 1mg / ml, and 2mg / ml.

[0038] Preparation of MTT solution: Weigh 0.2g of MTT powder, add 40ml of sterilized double distilled water, dissolve at 60°C, the concentration is 5mg / ml, sterilize by 0.2μm filter membrane, and store at 4°C.

[0039] Cell lines: human breast cancer cells MDA-MB-231, MDA-MB-468 cells and mouse breast cancer cell 4T1, all of which are reserved by the Key Laboratory of Natural Product Chemistry, Chinese Academy of Sciences, Guizhou Province. cells at 37°C, 5% CO 2 Cultured in DMEM medium containing 5% FBS in an incubator.

[0040] Collect logarithmic phase cells, MDA-MB-231 cells, MDA-MB-468 cells and 4T1 cells according to 6×10 3 Cells / well were inoculated in 96-well plates and cultured overnight. After the cells adhered to the wall, ...

Embodiment 2

[0046] Cell Growth Curve Determination

[0047] Collect logarithmic phase MDA-MB-231 cells and MDA-MB-468 cells respectively, according to 6×10 3 The density of cells / well was inoculated in 96-well plate, cultivated overnight, and after the cells adhered to the wall, different concentrations of sample solutions (0.25mg / ml, 0.5mg / ml, 1mg / ml, 2mg / ml) were added for treatment, respectively. OD 490nm was measured at 0h, 24h, 48h, 72h, and 96h, and the growth curve was drawn.

[0048] The result is as image 3 as well as Figure 4 As shown, compared with the control group, different concentrations of sample solutions can inhibit the proliferation of MDA-MB-231 cells and MDA-MB-468 cells, and with the increase of concentration, MDA-MB-231 cells and MDA-MB The growth rate of -468 cells gradually slowed down, indicating that the sample solution could inhibit the proliferation of MDA-MB-231 and MDA-MB-468 cells in a concentration- and time-dependent manner.

Embodiment 3

[0050] Apoptosis detection

[0051] MDA-MB-231 cells were divided into 4 × 10 5 The amount of cells / well was inoculated in a 60mm round petri dish for culture. After the cells adhered to the wall, different concentrations of sample solutions (0.5mg / ml, 1mg / ml, 2mg / ml) were added to act for 24h or 48h, and then collected cell. Wash 3 times with PBS, add 500 μl 1×Binding Buffer to resuspend the cells, add 5 μl AnnexinV-FITC and 10 μl propidium iodide, incubate at room temperature in the dark for 15 min, and calculate the cell apoptosis rate by flow cytometry.

[0052] The apoptosis experiment of MDA-MB-468 cells was the same as that of MDA-MB-231 cells.

[0053] The result is as Figure 5 As shown, Jingan Capsule has a significant effect on the apoptosis of human breast cancer cell MDA-MB-231.

[0054] The result is as Figure 6 As shown, Jingan Capsule has a significant effect on the apoptosis of human breast cancer cell MDA-MB-468.

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Abstract

The invention provides application of Jinxiaocapsule in preparation of anti-breast cancer drugs, and relates to the technical field of biological pharmacy. The application specifically relates to preparation of a medicinal preparation for inhibiting the activity of breast cancer cells, inhibiting the proliferation of the breast cancer cells and promoting the apoptosis of the breast cancer cells. The invention provides a pharmaceutical preparation for treating breast cancer. It is confirmed by the invention that the Jin-sensitive capsule can inhibit the activity of MDA-MB-231 cells, MDA-MB-468 cells and 4T1 cells, and has concentration dependence; the proliferation of MDA-MB-231 and MDA-MB-468 cells can be inhibited, and the concentration and time dependence is realized; the cell morphology of MDA-MB-231 and MDA-MB-468 can be changed, and the cell morphology of the influence on the apoptosis of human breast cancer cells MDA-MB-231 and MDA-MB-468 is obvious; and the cycle of human breast cancer cells MDA-MB-231 and MDA-MB-468 is retarded in the S period. And the Jinfei capsule is a clinical compound medicine and can be directly applied to a human body, and clinical safety evaluation is not needed.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to the application of Jingan capsules in the preparation of anti-breast cancer drugs. Background technique [0002] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the breast and is one of the most common malignant tumors in women worldwide. Every year, the number of new cases and deaths of breast cancer in China account for 12.2% and 9.6% of the world's respectively. Since the 1990s, the incidence of breast cancer in China has grown at more than twice the global rate, especially in urban areas. Currently, breast cancer is the most common cancer among Chinese women. Chemotherapy is one of the three basic methods of breast cancer treatment. Although many advances have been made in the chemotherapy of breast cancer, these anti-cancer drugs have serious side effects, which makes the treatment of breast cancer unable to achie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/355A61K9/48A61P35/00A61P15/14A61K31/167A61K31/13A61K31/4402
CPCA61K36/355A61K36/19A61K36/195A61K36/315A61K36/288A61K31/167A61K31/13A61K31/4402A61K9/48A61P35/00A61P15/14
Inventor 李艳梅杨珏邱剑飞张知音宋晶睿饶青黄磊李珂
Owner THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI
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