Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Porcine oocyte in-vitro maturation system containing butoxylic acid and application of porcine oocyte in-vitro maturation system

An oocyte and butylated hydroxy acid technology is applied in the field of in vitro maturation and culture of oocytes to achieve the effects of improving developmental efficiency, reducing apoptosis rate and reducing toxic and side effects

Active Publication Date: 2021-07-27
GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
View PDF11 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above studies are just some mechanism studies and research status in cells. Whether it can promote meiosis, improve oocyte development efficiency, reduce the apoptosis rate of oocyte development in vitro, and be used in the application of in vitro development of embryos no report yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Porcine oocyte in-vitro maturation system containing butoxylic acid and application of porcine oocyte in-vitro maturation system
  • Porcine oocyte in-vitro maturation system containing butoxylic acid and application of porcine oocyte in-vitro maturation system
  • Porcine oocyte in-vitro maturation system containing butoxylic acid and application of porcine oocyte in-vitro maturation system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The application of the porcine oocyte in vitro maturation system containing butadiene hydroxy acid comprises the following steps:

[0034] 1) Prepare oocyte maturation culture medium: 80% volume of TCM199, 10% volume porcine follicular fluid, 10% volume fetal bovine serum, 0.1g / L cysteine, 0.075g / L penicillin, 0.05g / L streptomycin, 10ng / mL insulin-like growth factor, 50ng / mL epidermal growth factor, 2.2g / LNaHCO 3 , 10IU / mL pregnant horse serum gonadotropin, 10IU / mL human chorionic gonadotropin, 20μmol / L butadiene hydroxy acid; the main components of the electrical activation solution are: 0.3mmol / L mannitol, 0.1mmol / LCaCl 2 ·H 2 O, 0.1mmol / L MgSO 4 ·7H 2 O, 0.5mmol / L Hepes, 0.01% (w / v) polyvinyl alcohol (PVA);

[0035] 2) Prepare embryo culture medium: 6.31g / LnaCl, 0.74g / LKCl, 0.047g / LKH 2 PO 4 , 2.1g / LNaHCO 3 , 0.022g / L sodium pyruvate, 0.62g / L calcium lactate, 0.15g / L L-glutamine, 0.55g / L hypotaurine, 20ml / L essential amino acid, 10ml / L non-essential amino aci...

Embodiment 2

[0045] The application of the porcine oocyte in vitro maturation system containing butyl hydroxy acid, compared with Example 1, the dosage of butyl hydroxy acid in step 1) is 10 μmol / L, and the others are the same as in Example 1.

Embodiment 3

[0047] The application of the porcine oocyte in vitro maturation system containing butadioxylic acid, compared with Example 1, the dosage of butadioxylic acid in step 1) is 30 μmol / L, and the others are the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a porcine oocyte in-vitro maturation system containing butoxylic acid and an application of the porcine oocyte in-vitro maturation system. A culture solution is prepared from a basic culture solution TCM-199, cysteine, penicillin, streptomycin, an insulin-like growth factor, an epidermal growth factor, fetal calf serum, pig follicle fluid, NaHCO3, pregnant mare serum gonadotropin, human chorionic gonadotropin and Bufexamac, and the apoptosis rate of in-vitro development of oocytes can be reduced; and the first polar body discharge rate, the parthenogenetic embryo cleavage rate, the blastocyst rate, the blastocyst total cell number, the in-vitro fertilization embryo cleavage rate, the blastocyst rate and the blastocyst total cell number are improved.

Description

【Technical field】 [0001] The invention relates to the technical field of in vitro maturation and culture of oocytes, in particular to an in vitro maturation system and application of porcine oocytes containing butyroxylic acid. 【Background technique】 [0002] Pigs are important model animals in biomedical research because their biological and genetic characteristics have high homology with humans, and are considered to be the animal of choice for the source of human xenogeneic organs. The effective combination of in vitro culture technology and today's rapidly developing gene editing technology not only promotes the development and application of embryo engineering, but also drives the development of the medical field. Oocyte in vitro maturation (IVM), as a key link in embryo engineering, has important application value in livestock breeding, resource protection and biological research. However, oocyte maturation still faces problems such as low efficiency and poor quality....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2500/32C12N2501/105C12N2501/11C12N2500/84C12N2501/31C12N2500/30
Inventor 吕玲燕吴柱月孙俊铭滕少花肖正中孙俊丽张家庆王献伟关志惠潘星辰
Owner GUANGXI ZHUANG AUTONOMOUS REGION INST OF ANIMAL HUSBANDRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com