Separation method of Colletotrichum musae
A technology of banana anthracnose and anthracnose bacteria, applied in the field of plant pathology, can solve the problems of time-consuming and labor-intensive, affecting the efficiency and effect of separation work, and achieve the effect of improving efficiency and simple separation operation
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[0035] 2. Preparation of Spore Discrete Silk
[0036] Take out the tip of a 1000μl ordinary pipette gun, put the tip of the tip on the flame and burn it, the point where the tip of the tip is ignited will melt, use an alcohol lamp fire extinguisher and other utensils to safely extinguish the fire on the tip, and use pointed tweezers Clamp a little melted tip of the gun and stretch it gently until it forms a hair-like filament. After cooling, cut off the excess filament and keep the filament about 3cm long connected to the tip of the gun to form a The spore discrete silk used to separate the spores of the banana anthracnose fungus, the discrete silk tool includes a sliding silk a and a silk stalk b part, such as figure 1 As shown, the two parts are naturally connected into one body, and the key technical function is to slide the silk a. Sliding filament a is small, soft and elastic.
[0037] When separating different specimens for use, you can wipe the sliding wire a with 75%...
Embodiment 1
[0059] Adopt a kind of separation method of banana anthracnose bacterium of the present invention, isolate the banana anthracnose bacterium of banana anthracnose standard sample 1, the separation and culture result is as follows Image 6 shown.
Embodiment 2
[0061] Adopt a kind of separation method of banana anthracnose bacterium of the present invention, isolate the banana anthracnose bacterium of banana anthracnose standard sample 2, separation and culture result is as follows Figure 7 shown.
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