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Separation method of Colletotrichum musae

A technology of banana anthracnose and anthracnose bacteria, applied in the field of plant pathology, can solve the problems of time-consuming and labor-intensive, affecting the efficiency and effect of separation work, and achieve the effect of improving efficiency and simple separation operation

Active Publication Date: 2021-07-27
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method needs to carry out the spore quantification and dilution operation process of the spore liquid, which is time-consuming and labor-intensive, and it is easy to be contaminated by bacteria, which will affect the efficiency and effect of the separation work.

Method used

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  • Separation method of Colletotrichum musae
  • Separation method of Colletotrichum musae
  • Separation method of Colletotrichum musae

Examples

Experimental program
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preparation example Construction

[0035] 2. Preparation of Spore Discrete Silk

[0036] Take out the tip of a 1000μl ordinary pipette gun, put the tip of the tip on the flame and burn it, the point where the tip of the tip is ignited will melt, use an alcohol lamp fire extinguisher and other utensils to safely extinguish the fire on the tip, and use pointed tweezers Clamp a little melted tip of the gun and stretch it gently until it forms a hair-like filament. After cooling, cut off the excess filament and keep the filament about 3cm long connected to the tip of the gun to form a The spore discrete silk used to separate the spores of the banana anthracnose fungus, the discrete silk tool includes a sliding silk a and a silk stalk b part, such as figure 1 As shown, the two parts are naturally connected into one body, and the key technical function is to slide the silk a. Sliding filament a is small, soft and elastic.

[0037] When separating different specimens for use, you can wipe the sliding wire a with 75%...

Embodiment 1

[0059] Adopt a kind of separation method of banana anthracnose bacterium of the present invention, isolate the banana anthracnose bacterium of banana anthracnose standard sample 1, the separation and culture result is as follows Image 6 shown.

Embodiment 2

[0061] Adopt a kind of separation method of banana anthracnose bacterium of the present invention, isolate the banana anthracnose bacterium of banana anthracnose standard sample 2, separation and culture result is as follows Figure 7 shown.

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Abstract

The invention discloses a method for separating Colletotrichum musae. According to the method, a special spore discrete filament is adopted, and a single conidium is directly separated from a Colletotrichum musae standard sample and is cultured to form a Colletotrichum musae germ strain with a single genetic composition. The implementation operation steps of the isolation culture are as follows: 1) preparation of a Colletotrichum musae standard sample; (2) preparing spore discrete filaments; (3) preparing a spore dispersing plate; (4) dispersing conidia; (5) cutting small square blocks loaded with monospores; and (6) forming a monospore isolated strain. The method has the following advantages: 1) a key appliance for separating spores is simple and easy to manufacture, and the separation operation is simple and easy; 2) single spore strains can be separated and obtained at one time; 3) the separation work consumes less time and labor; and 4) the working efficiency of Colletotrichum musae germ separation and the reliability of the separation result can be obviously improved.

Description

technical field [0001] The present invention relates to phytopathological techniques. Specifically, it is a method for isolating banana anthracnose bacteria. Background technique [0002] Banana anthracnose is a major disease in my country's banana industry, which is caused by the infection of banana anthracnose fungus (Colletotrichum musae). Carrying out biological research on banana anthracnose bacteria is an important basis for the prevention and control of banana anthracnose. The prerequisite for carrying out biological research on banana anthracnose bacteria is to have the materials of banana anthracnose bacteria. General laboratories often need to isolate banana anthracnose bacteria from disease standard samples. [0003] The genetic composition of the banana anthracnose bacterial population is complex, and the biological characteristics and pathogenicity of different strains are often quite different. Normal research usually needs to obtain materials of banana ant...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12N3/00C12R1/645
CPCC12N1/14C12N1/02C12N3/00
Inventor 张容博张君成王忠文
Owner GUANGXI UNIV
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