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Alkali-resistant compound microorganism pretreatment fungicide for pulping wheat straw and application of alkali-resistant compound microorganism pretreatment fungicide

A composite microorganism and pretreatment technology, which is applied in the use of microorganism/enzyme cellulose treatment, biological organic part treatment, microorganism-based method, etc. , achieve the effect of avoiding the use of chemical preparations, strong adaptability and universality, and high degree of industrialization

Active Publication Date: 2021-07-23
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although biopulping is a clean and environmentally friendly pulping process, the enzymes or microorganisms used in biodegradation limit the industrialization of biopulping
First of all, the cost of enzymes used in biodegradation is high, and the reuse rate is low, and the decomposition effect of using a single enzyme in biodegradation is not ideal, and a compound enzyme preparation is required, which further increases the production cost of biopulping; secondly, It is difficult to control the environmental conditions during the reproduction of microorganisms used for biodegradation, and it is easy to be polluted by miscellaneous bacteria and cannot achieve the purpose of biodegradation; moreover, the current pulp and paper industry mostly uses chemical pulping methods, and its production workshops and production equipment are all Compatible with the chemical pulping method, if the production process is directly replaced from the chemical pulping method to the biological pulping method, the cost is too high for pulp and paper companies and does not meet the actual production requirements

Method used

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  • Alkali-resistant compound microorganism pretreatment fungicide for pulping wheat straw and application of alkali-resistant compound microorganism pretreatment fungicide
  • Alkali-resistant compound microorganism pretreatment fungicide for pulping wheat straw and application of alkali-resistant compound microorganism pretreatment fungicide
  • Alkali-resistant compound microorganism pretreatment fungicide for pulping wheat straw and application of alkali-resistant compound microorganism pretreatment fungicide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Determination of the strain composition in the alkali-resistant compound microbial pretreatment bacterial agent:

[0067] (1) Obtain the source of bacteria: collect wheat straw waste and cultivated soil samples, soak them in 0.85% saline for 2-3 days, so that the microorganisms in the samples can be fully dispersed into the liquid environment;

[0068] (2) Enrichment culture: draw a certain volume of the bacteria-containing liquid in step (1) and transfer it to a complete culture medium (liquid) for 2-3 days, the culture temperature is 37°C, 180rpm;

[0069] (3) Dilution coating: After gradient dilution, draw 200 μL of diluted bacterial solution and evenly spread it on the complete medium (solid), culture for 1-2 days, and the culture temperature is 37°C;

[0070] (4) Liquid transfer: Pick a single different colony and transfer to complete medium (liquid), culture for 2-3 days, culture temperature is 37°C, 180rpm;

[0071] (5) Purification by streaking: Streak the bact...

Embodiment 2

[0078] An alkali-resistant composite microbial pretreatment bacterial agent for wheat straw pulping, the preparation method steps are as follows:

[0079] 1) Strain activation: inoculate Bacillus licheniformis, Alcaligenes faecalis and Bacillus lysine in YPD liquid medium, inoculate Bacillus subtilis and Bacillus cereus in LB liquid medium, 37°C, 180rpm Activation culture for 1 day;

[0080] 2) Bacteria liquid culture: transfer the bacterium liquid after step 1) activation culture to the corresponding liquid culture medium, 37 ℃, 180rpm expand culture for 1 day, the number of viable bacteria of the obtained Bacillus licheniformis bacterium liquid is 3.0×10 9 cfu / mL, the number of viable bacteria in Alcaligenes faecalis solution is 3.0×10 9 cfu / mL, the number of viable bacteria in the Bacillus lysinus solution is 2.0×10 10 cfu / mL, the number of viable bacteria in the Bacillus subtilis liquid is 2.0×10 9 cfu / mL, the number of viable bacteria in the Bacillus cereus liquid is 4...

Embodiment 3

[0091] An alkali-resistant composite microbial pretreatment bacterial agent for wheat straw pulping, the preparation method steps are as follows:

[0092] 1) Strain activation: inoculate Bacillus licheniformis, Alcaligenes faecalis and Bacillus lysine in YPD liquid medium, inoculate Bacillus subtilis and Bacillus cereus in LB liquid medium, 37°C, 180rpm Activation culture for 1 day;

[0093] 2) Bacteria liquid culture: transfer the bacterium liquid after step 1) activation culture to the corresponding liquid medium, 37 ℃, 180rpm expand culture for 2 days, the number of viable bacteria in the obtained Bacillus licheniformis bacterium liquid is 2.0×10 10 cfu / mL, the number of viable bacteria in Alcaligenes faecalis solution is 9.0×10 9 cfu / mL, the number of viable bacteria in the Bacillus lysinus solution is 8.0×10 10 cfu / mL, the number of viable bacteria in the Bacillus subtilis liquid is 2.0×10 10 cfu / mL, the number of viable bacteria in the Bacillus cereus liquid is 7.0×10...

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Abstract

The invention relates to an alkali-resistant compound microorganism pretreatment fungicide for pulping wheat straw and application of the alkali-resistant compound microorganism pretreatment fungicide. The alkali-resistant compound microorganism pretreatment fungicide comprises bacillus licheniformis, alcaligenes faecalis, Lysinibacillus sp., bacillus subtilis and bacillus cereus, the mass ratio of all bacterial strains to bacterial liquid is (1-3): (1-3): (1-2): (1-3): (1-3), in biological pretreatment of wheat straw, all the bacterial strains are mutually beneficial, symbiotic and synergistic interaction is achieved, and the efficient degradation of cellulose, hemicellulose and lignin is jointly promoted. According to the method, the biological pretreatment chemical pulping method is adopted for pulping, and the alkali-resistant compound microorganism pretreatment fungicide is used for biological pretreatment pulping, so that the use of a large amount of chemical preparations is avoided, the discharge of wastewater in the traditional chemical pulping process is reduced, the production cost of an enterprise is reduced, and the method is advanced in technology and environment-friendly at the same time.

Description

technical field [0001] The invention belongs to the technical field of pulping, and in particular relates to an alkali-resistant composite microbial pretreatment bacterial agent for pulping wheat straw and an application thereof. Background technique [0002] With the increasing demand for domestic paper in my country, the domestic paper industry is in short supply of wood raw materials, and there is an urgent need for raw materials that can replace daily paper. Non-wood fiber raw materials have the advantages of wide sources, high output, and low price in my country. If they are fully utilized by the pulp and paper industry, they can alleviate the pressure brought about by the shortage of wood raw materials. [0003] Papermaking is divided into two process stages, one is pulping, and the other is papermaking. Pulping is to separate the fibers from plant raw materials, and make slurry through washing, screening, crushing, and concentration; papermaking is to make pulp Dilut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20D21C5/00C12R1/10C12R1/05C12R1/01C12R1/125C12R1/085
CPCC12N1/20D21C5/005Y02W30/64
Inventor 黄迪王腾飞常德军武玉康
Owner QILU UNIV OF TECH
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