The application of the extract of chrysanthemum in the preparation of anti-oxidation and anti-tumor drugs

An anti-tumor drug, the technology of the yellow hairy ear grass, which is applied in the field of biomedicine and achieves the effects of simple preparation method, induction of apoptosis and easy operation.

Active Publication Date: 2022-04-29
GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still few researches on the pharmacological effects and the material basis of the efficacy of the yellow hair ear. Therefore, it is of great significance to carry out in-depth research and development on it, and to apply it to the preparation of antioxidant and antitumor drugs.

Method used

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  • The application of the extract of chrysanthemum in the preparation of anti-oxidation and anti-tumor drugs
  • The application of the extract of chrysanthemum in the preparation of anti-oxidation and anti-tumor drugs
  • The application of the extract of chrysanthemum in the preparation of anti-oxidation and anti-tumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of Fructus japonica extract

[0034] Grind the dried whole grass (1.0Kg), and extract with 80% (v / v) ethanol aqueous solution under continuous reflux for 3 times, each time for 2 hours, combine the extracts, concentrate to dryness under reduced pressure to obtain crude ethanol extract total extract (crude, 123.0g). The total extract (100.0g) was dispersed in water, extracted successively with an equal amount of petroleum ether, ethyl acetate and n-butanol, separated and concentrated under reduced pressure to constant weight to obtain petroleum ether extracts (PE fraction , 2.8g), ethyl acetate extract (EtOAc fraction, 3.58g) and n-butanol extract (BuOH fraction, 3.24g).

Embodiment 2

[0035] Example 2: Determination of Antioxidant Activity of Fructus Fructus Extract

[0036] Dissolve 1,1-diphenyl-2-[(2,4,6)-trinitro]phenylhydrazine (DPPH) (3.94 mg) in absolute ethanol to prepare a 0.4 mmol / L DPPH ethanol solution . The ethanol crude extract, petroleum ether extract, ethyl acetate extract and n-butanol extract were prepared into ethanol solutions with different concentration gradients (concentrations of ethanol crude extract were 50, 100, 150, 200, 250, 300 μg / mL ; The concentration of petroleum ether extract is 100, 150, 200, 250, 300, 350 μg / mL; the concentration of ethyl acetate extract is 100, 200, 300, 400, 500, 600 μg / mL; , 200, 300, 400, 500, 600 μg / mL). Take 1.5mL of each extract ethanol solution with different concentration gradients and the above prepared 0.4mmol / L DPPH ethanol solution and mix them in equal amounts at room temperature, react in the dark for 30min, and measure the concentration of each mixed solution with a UV spectrophotometer a...

Embodiment 3

[0041] Example 3: Determination of the inhibitory activity of the extract of Fructus japonica on the proliferation of tumor cells

[0042] MTT method was used to determine the effects of ethanol crude extract, petroleum ether extract, ethyl acetate extract and n-butanol extract on five kinds of human tumor cell lines (human breast cancer cell MDA-MB-231, colon cancer cell HCT-116, lung cancer cell line). Inhibitory effect on the proliferation of cell A549, cervical cancer cell Hela and oral epidermal cancer cell KB). DMSO was used as blank control, and 5-fluorouracil (5-FU) was used as positive control.

[0043] The above five tumor cells were divided into 3×10 3 Cells / well were seeded in 96-well plates in 5% CO 2Incubate at 37° C. for 24 hours in the presence of constant temperature. Then, the DMSO solutions of the above extracts (ethanol crude extract, petroleum ether extract, ethyl acetate extract and n-butanol extract) with concentrations of 6.25, 12.5, 25, 50, 100, 200...

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Abstract

The present invention discloses the application of the extract of A. chinensis in the preparation of anti-oxidation and anti-tumor medicaments. The extract of the present invention is prepared by the following method: pulverizing the grass, performing continuous reflux extraction with an ethanol aqueous solution, and concentrating the extract to obtain a crude ethanol extract; taking the crude ethanol extract and dispersing it in water , extracted with petroleum ether, ethyl acetate and n-butanol in turn, separated each extract and concentrated to constant weight to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively. The yellow hairy ear grass extract of the present invention has better antioxidative effect and antitumor effect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the application of the extract of Fructus pachyrhiza in the preparation of anti-oxidation and anti-tumor drugs. Background technique [0002] Although many important advances in early screening and treatment have been made in recent years, cancer is still the second leading cause of death after cardiovascular disease. Cancer morbidity and mortality are also showing a rapid increase. Existing chemotherapeutic drugs often cause various serious side effects while exerting therapeutic effects. At the same time, easy drug resistance also greatly affects the effect of chemotherapy drugs. Therefore, finding new antitumor drugs with high efficiency and low toxicity has always been an important field of drug research. [0003] Chinese herbal medicines and natural medicines have been an important source of drug discovery and development since ancient times. More and mor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/748A61P39/06A61P35/00
CPCA61K36/748A61P39/06A61P35/00A61K2236/333A61K2236/39A61K2236/51
Inventor 季红张超张静李山诺陈美鸿谭娅玲陈乐贤
Owner GUANGZHOU MEDICAL UNIV
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