High performance liquid chromatography detection method for anthocyanin in blueberries
A high-performance liquid chromatography and detection method technology, applied in the field of photoelectrode materials, can solve problems such as affecting analysis results and difficult separation, and achieve the effects of simple and rapid operation, optimized separation conditions, and low quantitative limit
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Embodiment 1
[0104] 1. Materials and reagents: The blueberries tested were collected from the blueberry planting base in Majiang County, Kaili City, Guizhou Province, and the blueberry variety is the rabbit eye series. Reagents include chromatographically pure acetonitrile, methanol, analytically pure formic acid, acetic acid, phosphoric acid, ethanol, citric acid, ethyl acetate
[0105] 2. Instruments and equipment: high performance liquid chromatography system (Agilent1260 liquid chromatograph, equipped with 1260 diode array detector, American Agilent Company); LGJ-50F vacuum freeze dryer (Beijing Songyuan Huaxing Technology Development Co., Ltd.); BEATL-10 Ultrapure water preparation instrument (Shandong Tinglan Environmental Protection Technology Co., Ltd.); in addition, it also involves rotary evaporator, ultrasonic cleaning instrument, digital display constant temperature water bath, and ultrafiltration device.
[0106] Three, the preparation of blueberry anthocyanin extract:
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Embodiment 2
[0144] Take 10mg of blueberry anthocyanin powder, dissolve it with 30wt% methanol solution and dilute to 10mL to obtain a sample solution, filter the sample solution with a 0.22 μm organic filter membrane to obtain a test solution for later use.
[0145] The liquid to be tested is detected by the same high-performance liquid chromatography detection conditions as in Example 1, and the high-performance liquid chromatography spectrum obtained figure 2 , figure 2 1 is delphinidin-3-O-glucoside, 2 is cyanidin-3-O-glucoside, 3 is petunia-3-O-glucoside, 4 is pelargonin-3 -O-glucoside, 5 is paeoniflorin-3-O-glucoside, and 6 is mallow pigment-3-O-glucoside.
[0146] Calculate the content of 6 main anthocyanins in the blueberry anthocyanin powder according to the standard equation provided in Example 1, the results are shown in Table 8, by figure 2 As can be seen from Table 8, Example 2 has achieved the determination of 6 main anthocyanins in blueberry anthocyanin powder through o...
Embodiment 3
[0150] Take an appropriate amount of blueberry red wine and filter it with a 0.22 μm organic filter to obtain the test solution for later use.
[0151] Using the same high-performance liquid chromatography detection conditions as in Example 1, 60 μL of the liquid to be tested was injected for detection, and the obtained high-performance liquid chromatography image 3 , image 3 1 is delphinidin-3-O-glucoside, 2 is cyanidin-3-O-glucoside, 3 is petunia-3-O-glucoside, 4 is pelargonin-3 -O-glucoside, 5 is paeoniflorin-3-O-glucoside, and 6 is mallow pigment-3-O-glucoside.
[0152] Calculate the content of 6 main anthocyanins in blueberry red wine according to the standard equation that embodiment 1 provides, and the results are shown in table 3, by image 3 As can be seen from Table 9, Example 3 achieves the determination of five main anthocyanins in blueberry red wine through one detection. It can be seen from Table 3 that the content of mallow pigment-3-O-glucoside in blueberry ...
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