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Inhibin B enzyme-linked immunosorbent assay kit and inhibin B detection method

An enzyme-linked immunosorbent assay and inhibin technology, which is applied in biological testing, measuring devices, material testing products, etc., can solve the problems of narrow detection linearity, weak anti-interference ability, and low sensitivity, achieve accurate and sensitive detection, and improve specificity. , a strong specific effect

Active Publication Date: 2016-04-27
深圳华康生物医学工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide an inhibin B enzyme-linked immunoassay kit and inhibin B testing method to overcome the long time-consuming, narrow detection linearity and low sensitivity of the existing inhibin B testing methods , weak anti-interference ability, etc.

Method used

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  • Inhibin B enzyme-linked immunosorbent assay kit and inhibin B detection method
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  • Inhibin B enzyme-linked immunosorbent assay kit and inhibin B detection method

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Embodiment 1

[0054] An enzyme-linked immunoassay kit for inhibin B, which includes the following reagents and components:

[0055] (1) A microtiter plate coated with an anti-inhibin B specific antibody;

[0056] (2) ELISA plate support;

[0057] (3) 100ng / L~0ng / L series inhibin B standard products;

[0058] (4) Sample treatment solution;

[0059] (5) Biotin-labeled anti-inhibin B antibody;

[0060] (6) horseradish peroxidase-labeled avidin;

[0061] (7) The formula of concentrated washing solution is: sodium chloride 8g, potassium dihydrogen phosphate 0.2g, disodium hydrogen phosphate 3g, potassium chloride 5g, Tween-202mL, distilled water 20mL;

[0062] (8) Color-developing solution: including color-developing solution A and color-developing solution B; wherein, formula of color-developing solution A: carbamide peroxide 1g, 10.3g citric acid, 35.8gNa 2 HPO 4 12H 2 O, Tween-20 100μL, distilled water 1000mL, pH=5; formula of chromogenic solution B: tetramethylbenzidine (TMB) 700mg (d...

Embodiment 2

[0075] A method for detecting inhibin B, comprising the steps of:

[0076] The obtained sample to be tested is detected using the enzyme-linked immunoassay kit for inhibin B in the above-mentioned embodiment 1, specifically, the enzyme-linked immunoassay kit for inhibin B in the above-mentioned embodiment 1 is used for detection The operations and results are calculated as follows:

[0077]Unpack the vacuum packaging bag and take out the microtiter plate, equilibrate at room temperature for 30 minutes, and set aside. Dissolve 1000ng / L, 500ng / L, 250ng / L, 60ng / L, 10ng / L, 0ng / L inhibin B calibrator in 1mL purified water, add 50uL to each well, and add 50uL of the sample to be tested to In each well; add 50uL sample treatment solution to each well, incubate at 37°C for 120 minutes; pour out the liquid in the well, wash 5 times with the diluted concentrated washing solution, turn the microplate upside down on absorbent paper and pat dry ; Add 100uL of biotin-labeled anti-inhibin ...

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Abstract

The invention discloses an inhibin B enzyme-linked immunosorbent assay kit and an inhibin B detection method. The inhibin B enzyme-linked immunosorbent assay kit includes an ELISA plate coated with an anti inhibin B beta-subunit antibody, a biotin-labeled secondary antibody, and an avidin-labeled enzyme marker; a coating antigen used for preparation of the ELISA plate coated with the anti inhibin B beta-subunit antibody is a specific antibody aiming at an inhibitor B beta subunit; the biotin labeled secondary antibody is a biotin-labeled inhibin B alpha-subunit monoclonal antibody. The inhibin B detection method comprises that the inhibin B enzyme-linked immunosorbent assay kit is used for detection of the inhibin B. Therefore, the inhibin B enzyme-linked immunosorbent assay kit and the inhibin B detection method have the characteristics of high sensitivity, high specificity, time saving and the like, and can be applied to clinical medical batch detection of the inhibin B.

Description

technical field [0001] The invention belongs to the technical field of detection kits, and in particular relates to an inhibin B ELISA detection kit and an inhibin B testing method. Background technique [0002] Inhibin B is secreted by reproductive system cells and is closely related to reproductive ability. It has endocrine, paracrine and autocrine regulatory effects on reproductive function. It has been confirmed that inhibin B is the main marker of ovarian reserve function and testicular seminiferous tubule function, and can be used for the detection of female infertility caused by ovarian factors and male infertility caused by seminiferous tubule dysfunction. [0003] At present, conventional enzyme-linked immunosorbent assay (ELISA) is used for the detection of inhibin B. The specific operation is as follows: firstly, add a pre-coated microwell plate to the sample, after incubation, wash several times, and then add the secondary antibody enzyme label And color develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/4704
Inventor 蔡华傅剑华曾周祥蔡栋操美芳李明磊邹玮
Owner 深圳华康生物医学工程有限公司
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