Papillary thyroid carcinoma biomarker and application thereof

A biomarker, papillary cancer technology, applied in the field of biotechnology and oncology, can solve the problem that the mechanism of PIM1 is not fully elucidated, and achieve the effect of improving efficacy and accuracy

Pending Publication Date: 2021-05-28
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The substrates of PIM1 that have been shown so far include BAD, Skp-2, p21AWF, CXCR4, STAT3, Eif4B and RUNX3, etc., but the mechanism of action of PIM1 has not been fully elucidated

Method used

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  • Papillary thyroid carcinoma biomarker and application thereof
  • Papillary thyroid carcinoma biomarker and application thereof
  • Papillary thyroid carcinoma biomarker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: Cell Culture

[0056] Nthy-Ori-3 cells (normal thyroid cell line), BCPAP cells (mutant papillary thyroid cancer cells) and TPC-1 cells (wild-type papillary thyroid cancer cells) were purchased from Cell Center, Shanghai Institute of Biological Sciences, Chinese Academy of Sciences resource. Nthy-Ori-3 and BCPAP cell lines were placed in RPMI-1640 (Gibco, U.S.) medium, 10% fetal bovine serum (Gibco, Carlsad, CA) was added to the culture medium, and placed in 37°C, 5% CO 2 Incubate in a humidified incubator.

Embodiment 2

[0057] Example 2: Transfection of small interfering RNA

[0058] The NOX4 and PIM1 siRNA sequences and their negative controls (Jiangsu, China, Biomaike Biotechnology Co., Ltd.) were designed and obtained, as shown below. use 3000 Transfection Kit (Thermo Fisher Science) was incubated for 6 h, and then mixed with respective siRNA oligonucleotides or corresponding negative controls. After transfection, place all cells in 5% CO 2 Incubate for 48 hours in a 37°C incubator.

[0059] NOX4 siRNA sequence:

[0060] Positive: 5'-(CAUCUGUUCUUAACCUCAA)dTdT-3',

[0061] Anti: 5'-(UUGAGGUUAAGAACAGAUG)dTdT-3';

[0062] PIM1 siRNA sequence:

[0063] Positive: 5'-(CAAGAUCUCUUCGACUUCA)dTdT-3',

[0064] Reverse: 5'-(UGAAGUCGAAGAGAUCUUG)dTdT-3'.

Embodiment 3

[0065] Embodiment 3: SGI-1776 treatment

[0066] SGI-1776 (Selleckchem) was dissolved in DMSO to prepare a SGI-1776 solution with a concentration of 5 mmol / L, and the prepared SGI-1776 solution was stored in a -80°C refrigerator for future use.

[0067] BCPAP and TPC1 cells were treated with SGI-1776 solutions at a final concentration of 2.5 μM and 5 μM for 24 hours, 48 ​​hours and 72 hours, respectively, and SGI-1776 with a final concentration of 0 μM was used as a control group. In the experiment, the highest concentration of dimethyl sulfoxide in the medium that has no significant effect on cell viability is 0.05%.

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Abstract

The invention relates to a papillary thyroid carcinoma biomarker and an application of a PIM1 gene inhibitor in preparation of a papillary thyroid carcinoma resisting preparation. The biomarker is a PIM1 gene and an expression product thereof. The invention researches the effect of the PIM1 gene in the pathological development process of PTC, and finds that the PIM1 possibly plays an important carcinogenic role in the occurrence and development of PTC, and the PIM1 is remarkably related to the expression of NOX4, that is, the PIM1 is regulated by NOX4 in PTC, so that the effect of the PIM1 in oxidative damage is played. Therefore, the biomarker has important significance and value for theoretical research on the pathogenesis of PTC, and the biomarker can be used as a marker for PTC diagnosis, so that the accuracy of PTC diagnosis is improved, and a theoretical basis is provided for research of PTC diagnosis products.

Description

technical field [0001] The invention relates to the fields of biotechnology and oncology, in particular to a biomarker of papillary thyroid carcinoma and its application. Background technique [0002] Papillary thyroid carcinoma (PTC) is one of the thyroid diseases, and it is relatively common in thyroid diseases, accounting for about 80% of thyroid cancers. Papillary thyroid cancer is more common in children or female patients under the age of 40. At the same time, papillary thyroid cancer grows slowly and can be localized in the thyroid for several years, but it can also spread from the source to the thyroid gland. Other parts of the gland or lymph node metastasis. [0003] In recent years, studies have found that PIM1 is an oncogene, which plays a role in promoting cancer in various tumors such as breast cancer, prostate cancer, skin cancer, esophageal cancer, and liver cancer. The proto-oncogene PIM1 is located on the long arm of chromosome 6 (6p21.1~p21.31), and its e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K31/7088A61P35/00
CPCC12Q1/6886A61K45/00A61K31/7088A61P35/00C12Q2600/158
Inventor 朱欣
Owner ZHEJIANG CANCER HOSPITAL
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