Biochemical analysis method for simultaneously detecting multiple target objects

A technology for biochemical analysis and target objects, applied in the field of biochemical analysis, can solve problems such as cumulative errors, lower analysis efficiency, and inability to realize simultaneous detection of multiple targets, and achieve the effects of fast speed, improved analysis efficiency, and fast recognition speed

Active Publication Date: 2021-05-28
HUAZHONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method enables ultrasensitive, absolute quantification of disease-associated proteins, but cannot simultaneously detect multiple targets
In addition, this method requires multiple magnetic separations and the addition of an acidic solution to elute the detection particles in the immune complex. The multi-step magnetic separation and washing process not only reduces the analysis efficiency, but also easily causes cumulative errors

Method used

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  • Biochemical analysis method for simultaneously detecting multiple target objects
  • Biochemical analysis method for simultaneously detecting multiple target objects
  • Biochemical analysis method for simultaneously detecting multiple target objects

Examples

Experimental program
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Effect test

Embodiment 1

[0064] Example 1 Detection of various inflammatory markers in blood by capillary separation

[0065] The sandwich immunoassay was used to detect four inflammatory markers interleukin 6 (IL-6), procalcitonin (PCT), C-reactive protein (CRP) and serum amyloid (SAA) in blood samples. its principle Figure 4Shown: PS microspheres with a particle size of 50 μm are used as the carrier, and the surface is modified with capture antibodies corresponding to IL-6, PCT, CRP, and SAA; 1 μm red PS microspheres, 1 μm blue PS microspheres, and 3 μm red PS microspheres are used. Microspheres and 3 μm blue PS microspheres are signal probes, which are coupled with detection antibodies corresponding to IL-6, PCT, CRP, and SAA, respectively. When the target exists, the capture antibody modified on the surface of the carrier, the target, and the detection antibody modified on the surface of the signal probe will form a sandwich complex, resulting in PS microspheres with a particle size of 50 μm and...

Embodiment 2

[0092] Example 2 Multivariate detection of Salmonella, Listeria monocytogenes and Staphylococcus aureus by composite membrane separation method

[0093] Multiple detection of Salmonella, Listeria monocytogenes and Staphylococcus aureus by DNA hybridization reaction, principle Figure 5 Shown: PS microspheres with a particle size of 50 μm are used as carriers, and the surface is modified with capture probes corresponding to Salmonella, Listeria monocytogenes, and Staphylococcus aureus; 1 μm, 3 μm, and 6 μm PS microspheres are used as signal probes , respectively coupled with detection probes corresponding to Salmonella, Listeria monocytogenes, and Staphylococcus aureus. When the target exists, the capture probe modified on the surface of the carrier, the target, and the detection probe modified on the surface of the signal probe will form a complex through DNA hybridization, resulting in PS microspheres with a particle size of 50 μm and different particles. The diameter of the...

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Abstract

The invention discloses a biochemical analysis method for simultaneously detecting multiple target objects, which combines biochemical reaction with visual microsphere counting, machine vision and deep learning, and respectively couples biological recognition molecules corresponding to the target objects to be detected on the surfaces of a carrier and a signal probe to perform immunoreaction or DNA molecular hybridization reaction. The method comprises the following steps: firstly, detecting a carrier and a signal probe, then separating the carrier and the signal probe by using a capillary tube or a composite film according to different particle sizes of the carrier and the signal probe, and finally, directly carrying out optical microscopic imaging counting on the separated signal probe. According to the invention, the signal probes with different particle sizes or colors are used for distinguishing different detection objects, so that simultaneous detection of multiple target objects is finally realized.

Description

technical field [0001] The invention belongs to the field of biochemical analysis and relates to a biochemical analysis method for simultaneously detecting multiple targets. Background technique [0002] With the development of the country and the improvement of people's living standards, the challenges faced in the fields of food safety, in vitro diagnostics and environmental monitoring are increasing day by day. Efficient detection methods have an extremely important impact on ensuring food safety, promoting human health, and ecological environment safety. . Most of the traditional detection methods rely on large-scale precision instruments and equipment. Although they have the characteristics of high precision and good accuracy, most of the instruments are expensive and inconvenient to carry. Before use, complex pre-treatment of the test samples is required. There are also stricter requirements for personnel. [0003] Immunoassay methods based on antibody-antigen recogn...

Claims

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Application Information

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IPC IPC(8): C12Q1/6834G01N33/546G01N33/566
CPCC12Q1/6834G01N33/54313G01N33/566C12Q2523/301C12Q2563/149C12Q2523/303
Inventor 陈翊平何慧禹赵维琦聂荣彬周阳
Owner HUAZHONG AGRICULTURAL UNIVERSITY
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