A kind of anti-opal film for inhibiting scar generation and its preparation method

A technology of anti-protein and stone film, which is applied in the direction of peptide/protein components, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems that the application has not been studied, and achieve the promotion of stable array growth, Accelerates wound healing and repair, and promotes the effect of directional cell growth

Active Publication Date: 2022-06-03
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these membranes are often used simply for cell induction, and their effects on cell growth and migration, especially in the application of inhibiting the scarring process, have not been investigated.

Method used

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  • A kind of anti-opal film for inhibiting scar generation and its preparation method
  • A kind of anti-opal film for inhibiting scar generation and its preparation method
  • A kind of anti-opal film for inhibiting scar generation and its preparation method

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preparation example Construction

[0032] The invention provides a preparation method of an inverse opal film for inhibiting the formation of scars, comprising the steps of:

[0033] 1) Preparation of an inverse opal film: a negative replication method is used to prepare an inverse opal film by using silica particles and polylactic acid-glycolic acid copolymer;

[0034] 2) Preparation of an inverse opal film that inhibits the formation of scars: using the inverse opal film obtained in step 1), uniformly stretched and then dried to obtain a stretched inverse opal film and then loaded with methyl groups in the stretched nano-holes (in the figure). Acrylic anhydride gelatin, antimicrobial peptide and vascular endothelial growth factor cocktail.

[0035] Further, the specific preparation steps of the negative replication method in step 1) are:

[0036] ① Use silica particles to prepare colloidal crystal templates;

[0037] (2) The polylactic acid-glycolic acid copolymer solution is used to penetrate the interior ...

Embodiment 1

[0047] A stretched inverse opal film, the preparation process is as follows figure 1 shown, the steps are as follows:

[0048] (1) Preparation of silica colloidal crystal template:

[0049] Silica particles with a diameter of 400 nanometers were selected, suspended in absolute ethanol, fully dispersed in absolute ethanol by vortexing and ultrasonication, and a silica solution with a concentration of 2% was prepared. Insert the glass sheet vertically into the silica solution, place it in a constant temperature and humidity box, and adjust the temperature inside the box to 30 degrees Celsius and the humidity to 40%. With the evaporation of absolute ethanol, the silica particles are neatly arranged on the surface of the glass sheet to form a silica colloidal crystal template.

[0050] (2) Preparation of inverse opal film

[0051]Prime, configure 15% polylactic acid-glycolic acid copolymer ethyl acetate solution, seal and place it away from light. Place a foam box in a fume ho...

Embodiment 2

[0055] Stretched inverse opal membranes are used to guide cell directional growth and alignment:

[0056] Take the unstretched inverse opal membrane prepared in Example 1, the inverse opal membrane stretched three times the original length, the inverse opal membrane stretched six times the original length, and the glass cell slides, and soaked in 75% ethanol solution , placed under UV light for 1 hour, rinsed 3 times with sterile PBS buffer, and then plated in six-well plates, and added 2ml of mouse embryonic fibroblast cell line NIH-3T3 cell suspension (4* 10 4 cells / ml) for 48 hours in a cell culture at a temperature of 37 degrees Celsius and a carbon dioxide concentration of 5%. Rinse twice with sterile PBS buffer to remove dead cells, stain the cells with 2uM calcein solution, remove excess dye after 30 minutes, rinse once with sterile PBS buffer and observe NIH-3T3 cells with a fluorescent inverted microscope Morphology and arrangement of cells grown on different substr...

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Abstract

The invention discloses an inverse opal film for inhibiting scar formation and a preparation method thereof. The method utilizes silica particles and poly(lactic-co-glycolic acid) copolymers to prepare a regular and interconnected porous structure through a negative replication method. Inverse opal film; Stretching at the glass transition temperature of poly(lactic acid-glycolic acid copolymer) to obtain a stretched inverse opal film with adjustable patterns, loaded with methacrylic anhydride gelatin and antimicrobial peptides by vacuum negative pressure. The inverse opal membrane prepared by the invention has good biocompatibility, has the function of guiding the directional growth and orderly arrangement of cells, and inhibits the formation of scars at the wound site, and the antibacterial peptide loaded in its porous structure can be slowly released to achieve a strong antibacterial effect function, so as to accelerate the healing of the wound, with various functions and simple preparation.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an inverse opal film for inhibiting scar formation and a preparation method thereof. Background technique [0002] Scar tissue formation often occurs in severe burns, skin trauma, or as a complication of surgical intervention. Hypertrophic scars are primarily caused by persistent dermal fibrosis, irregular collagen deposition, and hyperproliferative hypertrophic scar fibroblasts. The formation of scar tissue not only affects the appearance, too much scar tissue can also affect the flexible movement of the joint. Conventional treatments for hypertrophic scars can be divided into non-invasive treatments (eg, topical silicone sheets and pressure dressings) and invasive treatments (eg, surgical excision and laser ablation). However, these treatments have adverse reactions such as low efficacy, high recurrence rate, and painful process. How to effectively prevent the f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61K47/42A61K47/24A61K47/04A61K9/70A61P17/02A61P31/04
CPCA61K38/02A61K47/42A61K47/24A61K47/02A61K9/7007A61P17/02A61P31/04
Inventor 赵远锦翁万青王月桐施可庆张纯武池俊杰
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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