Bacillus peoriae and application thereof in prevention and treatment of citrus canker
A technology of Bacillus russica and Bacillus, applied in the field of Bacillus russica and its application in the prevention and treatment of citrus canker, can solve the problems of slow progress, weak research on citrus canker control measures, and lack of innovation.
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[0060] 1. Screening of strains
[0061] 1.1 Material preparation
[0062] Sample sampling: The soil sampling site is Guangxi Nonggang National Nature Reserve. The surface soil was removed, and soil samples at a depth of 15cm below the surface were collected, stored in a 4°C incubator, and brought back to the laboratory for further processing.
[0063] Separation and purification medium:
[0064] LB liquid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, distilled water 1000mL.
[0065] LB solid medium: tryptone 10.0g, yeast powder 5.0g, sodium chloride 5.0g, agar powder 15g, distilled water 1000mL.
[0066] PDA medium: 200.0g potato, 20.0g glucose, 20.0g agar, 1000mL distilled water.
[0067] 1.2 Isolation, purification and screening of strains
[0068] 1.2.1 Separation and purification
[0069] Add 2g of soil sample to 18ml of sterile water to prepare soil suspension, shake it on a shaker (180r / min) for 30min, and dilute it to 10% with sterile water. -1 ...
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