Russula virescens symbiotic fungus M2-1 as well as fungicide and fermentation liquor extract thereof
A technology of symbiotic fungi and cyanobacteria, applied in the field of microorganisms, can solve the problems of lack of antibacterial activity cyanobacteria symbiotic fungi and the like
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Embodiment 1
[0042] Such as Figure 1 to Figure 9 As shown, the present invention screens and obtains a kind of symbiotic fungus M2-1 (Mucorfragilis brittle mold) of P. of. The fermentation liquid extract of its strain culture is pathogenic to Bacillus lentus, Vibrio parahaemolyticus, Staphylococcus hemolyticus, Pseudomonas aeruginosa, Shigella flexneri, Bacillus subtilis, Escherichia coli, etc. Bacteria have good antibacterial activity. In order to alleviate the threat of pathogenic bacteria drug resistance to human health, research and development of new antibacterial drugs provide a new way.
[0043] Isolation and identification of the symbiont M2-1 strain of Pleurotus chinensis
[0044] 1. Isolation of Symbiotic Bacteria of Pseudomonas
[0045] Rinse the fruiting body of Pleurotus chinensis with tap water earlier for 36 hours, then transfer to the aseptic workbench, and then rinse the fruiting body with 3L sterile water.
[0046] Use a sterilized knife to take 2g of Pleurotus spor...
Embodiment 2
[0058] Extraction of Active Substances from Symbiotic Bacteria M2-1
[0059] 1. Liquid fermentation culture
[0060] Liquid fermentation medium LMM: lactose 15g / L, sodium nitrate 6g / L, potassium chloride 0.52g / L, magnesium sulfate heptahydrate 0.52g / L, potassium dihydrogen phosphate 1.52g / L, trace elements 1ml / L, pure Water 1L. Media aliquots.
[0061] Add 700lul of MY liquid to a 2ml centrifuge tube, then scrape an appropriate amount of mycelium of Pleurotus chinensis M2-1 into the centrifuge tube, crush it for 90 seconds, and then inoculate the crushed solution into each tube Place in a Erlenmeyer flask with LMM liquid medium in a constant temperature shaking incubator at 150r min-1, and culture at 28°C for 10 days
[0062] 2. Extraction of cultured mycelium
[0063] After the fermentation culture is completed, the obtained fungal mycelium and culture solution. Use a separatory funnel to separate the mycelium and the bacterial liquid, add ethyl acetate (1:1) to the bact...
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