Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nano-enzyme and preparation method thereof and application thereof in determination of 5-hydroxytryptamine

A technology of hydroxytryptamine and nanozyme, which is applied in chemical instruments and methods, measuring devices, fluorescence/phosphorescence, etc., and can solve problems such as high price, expensive chromatography equipment, and long analysis cycle

Active Publication Date: 2021-04-30
SOUTHEAST UNIV
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the methods disclosed above, chromatography is the main analysis method, and chromatography not only requires expensive equipment but also has a long analysis period
In the methods disclosed above, catalyzed analysis by monoamine oxidase is an important means, but natural enzymes are easily inactivated, have a short shelf life, and are expensive

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano-enzyme and preparation method thereof and application thereof in determination of 5-hydroxytryptamine
  • Nano-enzyme and preparation method thereof and application thereof in determination of 5-hydroxytryptamine
  • Nano-enzyme and preparation method thereof and application thereof in determination of 5-hydroxytryptamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of nanozyme RF Cu Tb DDE

[0027] Dissolve 1.5 mg riboflavin (RF) in 10 mL water and heat to 80°C to dissolve, add 1 mL 10 mM Tb(NO 3 ) 3 aqueous solution and 1 mL of 10 mM Cu(NO 3 ) 2 Add 4 mL of ethylene glycol solution containing 0.04 g of polyvinylpyrrolidone (PVP, molecular weight: 40,000), and add 4 mL of 5 mM 4,4-dicarboxydiphenyl ether DMF solution to form terbium ion: copper ion: nucleus The molar ratio of flavin: 4, 4-dicarboxydiphenyl ether is a mixture of 1:1:0.4:2. After stirring vigorously for 20 minutes, the mixture was transferred to the reaction kettle, reacted at 160 °C for 6 hours, cooled to room temperature (25 °C), collected the precipitate by centrifugation, washed with ethanol for 3 times, and dried in an oven at 80 °C to obtain nanozyme. figure 1 It is a scanning electron micrograph of the prepared nanozyme RF Cu Tb DDE, and the nanozyme RF Cu Tb DDE is a hollow spherical nanoparticle with a particle size of 50-300 nm. ...

Embodiment 2

[0028] Example 2 Nanozyme RF Cu Tb DDE Catalyzes 5-Hydroxytryptamine

[0029] Take 20 μL of 0.02 mM 5-hydroxytryptamine aqueous solution and 10 μL of the nanozyme RF Cu TbDDE suspension (0.2 mg / mL) prepared in Example 1, and add them to 970 μL of water samples respectively. UV-Vis and fluorescence spectra of the time-determined solutions. figure 2 It is the ultraviolet-visible spectrum of the solution at 0, 5, 10, 15, 20, 25, 30, 35, and 40 minutes of reaction. Under the catalysis of nanozyme RF Cu Tb DDE, the solution is at 220 nm, 276 nm, and 297 nm. The absorbance increased continuously with the reaction, and the peaks at 220 nm, 276 nm and 297 nm were the absorption peaks of 5-hydroxyindoleacetic acid, indicating the formation of 5-hydroxyindoleacetic acid. image 3 It is the fluorescence spectrogram of the solution at 0, 5, 10, 15, 20, 25, 30, 35, and 40 minutes. Under the catalysis of nanozyme RF Cu Tb DDE, the fluorescence intensity of the solution at 336 nm increases...

Embodiment 3

[0030] Example 3 Nanozyme RF Cu Tb DDE Luminescent Detection of 5-Hydroxytryptamine

[0031]Take 10 μL of the nanozyme RF Cu Tb DDE (0.3 mg / mL) prepared in Example 1 and add it to 980 μL of HEPES buffer solution (10 mM, pH 7). Add 10 μL of different concentrations of 5-hydroxytryptamine aqueous solution (containing 10% ethanol, v / v) to the above buffer to prepare 5-hydroxytryptamine containing 0, 0.05, 0.1, 0.2, 0.5 and 1.0 μM Standard solution, mix well and react for 30 minutes, measure Tb under 278 nm excitation 3+ Fluorescence intensity at 545 nm. Figure 5 is Tb 3+ Plot of fluorescence intensity versus 5-hydroxytryptamine concentration, Tb 3+ The fluorescence intensity is linearly proportional to the concentration of 5-hydroxytryptamine, and the detection sensitivity is 0.05 μM. The content of 5-hydroxytryptamine in the sample solution is obtained by using the linear relationship diagram and the measured fluorescence intensity of the 5-hydroxytryptamine sample solutio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Sensitivityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a nano-enzyme and a preparation method and application thereof in determination of 5-hydroxytryptamine. The nano-enzyme is a nano-material formed by terbium ions, copper ions, riboflavin and 4, 4-dicarboxyl diphenyl ether through solvothermal reaction. The nano-enzyme can catalyze and emit light to display neurotransmitter molecule serotonin. The nano-enzyme provided by the invention has high catalytic activity and stability and low cost, and can be applied to catalysis of reaction of 5-hydroxytryptamine and detection of 5-hydroxytryptamine in a sample.

Description

technical field [0001] The invention relates to a nanozyme, a preparation method thereof and an application in measuring 5-hydroxytryptamine, belonging to the technical field of catalysis and luminescence detection. Background technique [0002] 5-Hydroxytryptamine (5-HT), also known as serotonin, is an important monoamine neurotransmitter molecule. 5-Hydroxytryptamine is distributed in the central nervous system, peripheral tissues and blood, and participates in many physiological processes including endocrine regulation, sleep, emotion, appetite and so on. Low levels of 5-HT in the body may cause depression, anxiety neurosis, obsessive-compulsive disorder, high blood pressure, migraine, etc.; high levels of 5-HT may lead to serotonin syndrome, poisoning, and even death. 5-HT is an important biomarker, and its content in organisms, body fluids, and excreta plays an important role in the diagnosis, treatment, and pathological research of various diseases such as cancer, neu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64C09K11/06
CPCG01N21/6428G01N21/6486G01N21/6408C09K11/06C09K2211/182
Inventor 陈扬王莉
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products