Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain

A technology for producing Candida utilis and kitchen waste, which is applied in the fields of genetic engineering and fermentation engineering, can solve problems such as the influence of hydrolysis effect and the inability of protease to achieve the effect of action, and achieves the effect of good efficiency and large amino acid yield.

Pending Publication Date: 2021-04-20
GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the complexity of protein components in food waste, a single protease often cannot achieve a good effect, and multiple enzymes are required to work together, and the ratio of different enzymes will also have a great impact on the hydrolysis effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain
  • Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain
  • Candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste and construction method of candida utilis double-gene co-expression strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0044]The pMD19-X recombinant plasmid is obtained by recombining the amplified target gene with the pMD19-T vector, that is, the recombinant plasmid containing the target gene X, where X represents the pepA gene or pepF gene. Digest the recombinant plasmid containing pepA or pepF, and at the same time, digest the Candida utilis expression vectors pcGAPGA and pcTEF1GA respectively, and connect the expression vector pcGAPGA and the target gene pepA obtained after digestion to obtain the pcGAPGA-pepA recombinant plasmid , ligating the expression vector pcTEF1GA obtained after enzyme digestion and the target gene pepF to obtain the pcTEF1GA-pepF recombinant plasmid; pcGAPGA, the structure of pcTEF1GA and the construction process of the recombinant plasmid are as follows Figure 12 shown. After the pcTEF1GA-pepF recombinant plasmid was electrotransformed into Candida utilis, the recombinant Candida utilis containing the single gene of carboxypeptidase pepF was obtained, and the bio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of genetic engineering and fermentation engineering, and provides a candida utilis double-gene co-expression strain for hydrolyzing protein components in kitchen waste. The candida utilis double-gene co-expression strain is constructed by integrating carboxypeptidase and endoprotease genes onto a candida utilis genome through a candida utilis expression vector. According to the candida utilis double-gene co-expression strain capable of degrading the kitchen waste, and the candida utilis double-gene co-expression strain can specifically degrade the protein components in the kitchen waste and decompose and convert the protein components into small peptides and amino acids.

Description

technical field [0001] The invention relates to the fields of genetic engineering and fermentation engineering, and more specifically relates to a dual-gene co-expression strain of Candida utilis that hydrolyzes protein components in kitchen waste and a construction method thereof. Background technique [0002] The protein component in kitchen waste is the main component in kitchen waste except carbohydrates and fat. After the food waste is fermented, the microorganisms basically exhaust the carbohydrates in the food waste, but there are more protein substances left. Therefore, if the protein components in food waste are directly discharged into the environment, it is very easy to cause environmental pollution. How to convert and utilize protein in food waste is a difficult problem. [0003] Microbial fermentation is being valued by the industry at home and abroad. Fermentation technology is becoming an effective way to comprehensively utilize food waste, and truly achieve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/19C12N15/57C12N15/81C12R1/72
CPCC12P21/06C12N15/815C12N15/70C12N9/62C12Y304/23018C12Y304/24C12N9/50C12R2001/72C12N9/485C12N2800/102C12N2800/22
Inventor 刘泽寰林蒋海孙涛
Owner GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com