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Styela plicata-derived fungus and application thereof

A technology of fungi and sources, applied in the direction of medical raw materials derived from fungi, fungi, microorganisms, etc., can solve the problems of plant extract drug stability and quality uniformity, drug resistance, and unsatisfactory treatment effects, etc. Achieve good development prospects, not destroy the ecological balance, easy to achieve the effect of industrialization

Active Publication Date: 2021-04-13
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these drugs will cause side effects such as nausea, headache, diarrhea, insomnia, pain, hallucinations or dizziness to patients, and there are problems such as drug resistance and unsatisfactory treatment effect.
Chinese patent application CN111097010A discloses an extract for inhibiting acetylcholinesterase. The extract is extracted from the stems of Grape spp. Learning and memory behaviors in mouse models, but the drug stability and quality uniformity of plant extracts are difficult to guarantee, and there are still great limitations in practical applications

Method used

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  • Styela plicata-derived fungus and application thereof
  • Styela plicata-derived fungus and application thereof
  • Styela plicata-derived fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation and Identification of Ascidian-derived Fungal Strain Beauveria felina SYSU-MS7908

[0032] 1. Strain isolation

[0033] Sample: Styela plicata from North Reef, Xisha Islands, South China Sea.

[0034] Isolation method: Disinfect the surface of fresh sea squirts, dry them for a while, grind them, inoculate them into PDA medium under aseptic conditions, and culture them below 28°C for 5-7 days to obtain a single strain SYSU-MS7908; the obtained strain SYSU-MS7908 Preserve at 4°C on the slant of ordinary YPD medium.

[0035] 2. Morphological and physiological and biochemical identification

[0036] The obtained single strain SYSU-MS7908 was inoculated on YPD medium and cultured at a constant temperature of 26°C. The biological characteristics of the strain were as follows: there was white mycelium on the surface of the initial colony, and white slender conidiophores formed after a certain period of growth. Upright filaments (Synnemata), bottle-shaped...

Embodiment 2

[0042] Example 2 Fermentation of ascidian-derived fungal strain Beauveria felina SYSU-MS7908 and extraction, separation and purification of pyridine-containing new skeleton heteroterpenoids

[0043] 1. Preparation and separation of new pyridine-containing amphichoterpenoid compounds 1-3

[0044] 1.1 Fermentation was carried out with the ascidian-derived fungal strain Beauveria felina SYSU-MS7908, and the fermentation product was extracted, separated, and purified to obtain compounds 1-3.

[0045] (1) Seed cultivation:

[0046] Seed medium: 10g of yeast powder, 20g of peptone, 20g of glucose, 1L of tap water, evenly distributed in five 500mL Erlenmeyer flasks, extinguished at 115°C for 30 minutes.

[0047] Seed cultivation: Inoculate the ascidian-derived fungal strain Beauveria felina SYSU-MS7908 into the seed culture medium, and culture it on a shaker at 150 rpm at a temperature of 28°C for 96 hours to obtain a seed culture solution.

[0048] (2) Fermentation culture:

[00...

Embodiment 3

[0074] Example 3 Compound anti-acetylcholinesterase activity assay

[0075] 1. Experimental materials:

[0076] Experimental objects: 5 compounds prepared in Example 2;

[0077] Experimental materials: the substrate is acetylthiocholine iodide (Shanghai Aladdin); the chromogen is DTNB, 5,5,-dithiobis(2-nitrobenzoic acid) (Shanghai Aladdin); acetylcholinesterase (derived from electric eel, 500U purchased from sigma), Rivastigmine (Shanghai Dibo);

[0078] 0.1M phosphate buffer solution PBS with pH 7.6: Prepare 0.1M dipotassium hydrogen phosphate and potassium dihydrogen phosphate solutions respectively, then fill a beaker with a certain amount of dipotassium hydrogen phosphate solution, add potassium dihydrogen phosphate solution to it, and keep stirring. Adjust the pH to 7.6;

[0079] Substrate (iodothioacetylcholine, ATOH) concentration: 1.3mg / ml, chromogen DTNP: 1.8mg / mL; enzyme concentration: 0.9U / ml.

[0080] 2. Experimental method:

[0081] Determination of acetylcho...

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Abstract

The invention belongs to the technical field of fungus biological medicine application, and particularly relates to a styela plicata-derived fungus and application thereof. The styela plicata-derived fungus, Beauveria felina fungus strain SYSU-MS7908, can be metabolized to synthesize a pyridine-containing novel skeleton meroterpenoid compound--amphichoterpenoid. Experimental verification shows that the compound has remarkable acetylcholinesterase activity resistance, and has a good development prospect in the preparation of medicines for preventing and / or treating acetylcholinesterase-mediated diseases; and modern microbial fermentation engineering technology is used for reproduction, and worries about raw materials are avoided. Meanwhile, the invention has the advantages that ecological balance is not destroyed, industrialization is easy to achieve, etc. The styela plicata-derived fungus is a renewable drug resource with development prospects, and abundant raw materials are provided for research and development of marine microbial products and marine microbial drugs.

Description

technical field [0001] The invention belongs to the technical field of fungal biomedical application. More specifically, it relates to a sea squirt-derived fungus and its application. Background technique [0002] Alzheimer's disease (Alzheimer disease, AD) is a kind of central nervous system degenerative disease characterized by progressive cognitive dysfunction and behavioral damage, mainly occurs in the elderly, commonly known as senile dementia. With the acceleration of the aging society, AD has become an important threat to human health, which belongs to the category of clinical medical problems and major diseases. Studies have found that acetylcholinesterase inhibitors (AChEI) are the main anti-AD therapeutic drugs, mainly by inhibiting excessive acetylcholinesterase (AChE) activity and directly acting on the cholinergic system to achieve the effect of treating neurodegenerative diseases such as AD. Purpose. [0003] At present, about five drugs for the treatment of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18A61K36/06A61P25/28A61P25/18C12R1/645
CPCC12N1/14C12P17/188A61K36/06A61P25/28A61P25/18
Inventor 陈森华姜明华刘岚
Owner SUN YAT SEN UNIV
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