Styela plicata-derived fungus and application thereof
A technology of fungi and sources, applied in the direction of medical raw materials derived from fungi, fungi, microorganisms, etc., can solve the problems of plant extract drug stability and quality uniformity, drug resistance, and unsatisfactory treatment effects, etc. Achieve good development prospects, not destroy the ecological balance, easy to achieve the effect of industrialization
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Embodiment 1
[0031] Example 1 Isolation and Identification of Ascidian-derived Fungal Strain Beauveria felina SYSU-MS7908
[0032] 1. Strain isolation
[0033] Sample: Styela plicata from North Reef, Xisha Islands, South China Sea.
[0034] Isolation method: Disinfect the surface of fresh sea squirts, dry them for a while, grind them, inoculate them into PDA medium under aseptic conditions, and culture them below 28°C for 5-7 days to obtain a single strain SYSU-MS7908; the obtained strain SYSU-MS7908 Preserve at 4°C on the slant of ordinary YPD medium.
[0035] 2. Morphological and physiological and biochemical identification
[0036] The obtained single strain SYSU-MS7908 was inoculated on YPD medium and cultured at a constant temperature of 26°C. The biological characteristics of the strain were as follows: there was white mycelium on the surface of the initial colony, and white slender conidiophores formed after a certain period of growth. Upright filaments (Synnemata), bottle-shaped...
Embodiment 2
[0042] Example 2 Fermentation of ascidian-derived fungal strain Beauveria felina SYSU-MS7908 and extraction, separation and purification of pyridine-containing new skeleton heteroterpenoids
[0043] 1. Preparation and separation of new pyridine-containing amphichoterpenoid compounds 1-3
[0044] 1.1 Fermentation was carried out with the ascidian-derived fungal strain Beauveria felina SYSU-MS7908, and the fermentation product was extracted, separated, and purified to obtain compounds 1-3.
[0045] (1) Seed cultivation:
[0046] Seed medium: 10g of yeast powder, 20g of peptone, 20g of glucose, 1L of tap water, evenly distributed in five 500mL Erlenmeyer flasks, extinguished at 115°C for 30 minutes.
[0047] Seed cultivation: Inoculate the ascidian-derived fungal strain Beauveria felina SYSU-MS7908 into the seed culture medium, and culture it on a shaker at 150 rpm at a temperature of 28°C for 96 hours to obtain a seed culture solution.
[0048] (2) Fermentation culture:
[00...
Embodiment 3
[0074] Example 3 Compound anti-acetylcholinesterase activity assay
[0075] 1. Experimental materials:
[0076] Experimental objects: 5 compounds prepared in Example 2;
[0077] Experimental materials: the substrate is acetylthiocholine iodide (Shanghai Aladdin); the chromogen is DTNB, 5,5,-dithiobis(2-nitrobenzoic acid) (Shanghai Aladdin); acetylcholinesterase (derived from electric eel, 500U purchased from sigma), Rivastigmine (Shanghai Dibo);
[0078] 0.1M phosphate buffer solution PBS with pH 7.6: Prepare 0.1M dipotassium hydrogen phosphate and potassium dihydrogen phosphate solutions respectively, then fill a beaker with a certain amount of dipotassium hydrogen phosphate solution, add potassium dihydrogen phosphate solution to it, and keep stirring. Adjust the pH to 7.6;
[0079] Substrate (iodothioacetylcholine, ATOH) concentration: 1.3mg / ml, chromogen DTNP: 1.8mg / mL; enzyme concentration: 0.9U / ml.
[0080] 2. Experimental method:
[0081] Determination of acetylcho...
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