A method for extracting melanin from cuttlefish juice

A technology of cuttlefish juice and melanin, which is applied in the field of pigment extraction technology, can solve the problems of impact differentiation, etc., and achieve the effect of retaining integrity, good extraction rate and simple operation

Active Publication Date: 2022-03-29
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, the raw material also has an important influence on the type of extracted substance (eumelanin or phenomelanin) obtained at last, which will affect the subsequent distinction

Method used

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  • A method for extracting melanin from cuttlefish juice
  • A method for extracting melanin from cuttlefish juice
  • A method for extracting melanin from cuttlefish juice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Extract melanin by alkali extraction and acid precipitation method, weigh 5.0g of cuttlefish juice powder, add 0.5-2.5mol / L NaOH solution according to the solid-to-liquid ratio of 1:10-50 (g / mL), and place it in a 30-70℃ After leaching in a water bath for 1-3h, 8000r·min －1 Centrifuge for 10min, take the supernatant, use 6.00mol / L HCl solution to adjust the pH value to 1-3, after standing, 10000r·min －1 Centrifuge for 15min, take the precipitate and wash it with water, 15000r·min －1 Centrifuge for 5 min, repeat the operation 3 times, freeze-dry the precipitate, and calculate the extraction rate (see Table 1-5).

[0028] Table 1 Effect of NaOH concentration on the extraction rate of melanin

[0029] NaOH concentration (mol / L) Grams extracted (g) Extraction rate (%) 0.5 0.8609 17.22 1.0 1.1299 22.60 1.5 0.7293 14.59 2.0 0.6327 12.65 2.5 0.3467 6.93

[0030] Note: The solid-to-liquid ratio is 1:20, the temperature is 50°C,...

Embodiment 2

[0052] The raw material pretreatment is the same as in Example 1 to obtain dry cuttlefish juice powder. Take alkaline protease to extract melanin as an example.

[0053] After mixing the squid juice powder and the alkaline solution with a pH value of 9-11 according to the ratio of solid to liquid (g / ml) of 1:3-100, add alkaline protease, the amount of enzyme added is 60,000-180,000 U / g, Carry out enzymatic hydrolysis at 40-60°C for 3-5h, after the reaction, use 6000r·min -1 Centrifuge for 10 min. After centrifugation, remove the supernatant, wash the precipitate with distilled water and centrifuge, and repeat the operation 3 times to obtain a melanin precipitate, freeze it in a -80°C ultra-low temperature freezer, and freeze-dry it in a vacuum freeze dryer to obtain a melanin powder. After freeze-drying, calculate the extraction rate (see Table 8-12), and store in a 4°C refrigerator for later use.

[0054] The impact of table 8 alkaline protease enzyme amount on melanin extr...

Embodiment 3

[0082] The verification research of the melanin that embodiment 3 extracts

[0083] 1. Carry out infrared spectroscopic analysis with the obtained melanin and the standard substance extracted from the above-mentioned embodiment:

[0084] Weigh the purchased standard samples, enzyme-extracted melanin samples, and alkali-extracted melanin samples that have been fully ground, place them in the center of a clean disc, rotate and compress the samples, and use a Fourier transform infrared spectrometer to detect the infrared spectra of the samples. . (see Picture 1-1 , 1-2, 1-3)

[0085] 2. Carry out the ultraviolet maximum absorption wavelength scanning of the obtained melanin and the standard substance extracted from the above examples:

[0086] The ultraviolet-visible absorption spectrum has a high degree of specificity, that is, the energy absorbed by molecules of a certain structure is certain, and the change of the structure can be judged by studying the changes of the ultra...

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Abstract

The invention relates to the field of pigment extraction technology, in particular to a method for extracting melanin from cuttlefish juice by using enzymes. Specifically, the cuttlefish juice is filtered to remove impurities, dried or freeze-dried to obtain powder, and the melanin is extracted with enzymes. The ink melanin obtained by the method of the invention has high extraction rate and good purity. The method is simple to operate, environmentally friendly and efficient, and is suitable for large-scale operation of extracting high-purity melanin from cuttlefish juice.

Description

technical field [0001] The invention relates to the field of pigment extraction technology, in particular to a method for extracting melanin from cuttlefish juice by using enzymes. Background technique [0002] Melanin is an important class of natural pigment widely present in nature, mainly distributed in animals, plants and microorganisms. Melanin is a kind of macromolecular substances with complex and diverse structures polymerized by polyhydroxyindole or polyhydroxyphenol. It is often combined with proteins and other substances and exists in cells. There are two types of animal melanin commonly found in nature, one is eumelanin and the other is demelanin. Different species have different differences in melanin. The scientific name of cuttlefish is also squid, and the melanin in the ink sac is mainly eumelanin. Eumelanin is a two-molecular indole structure formed by the oxidation of tyrosine, namely DHI (5,6-dihydroxyindole) and DHICA (5,6-dihydroxyindole acid), so mel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 邢荣娥宋雯杨皓月刘松于华华李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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