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Core-shell structure microsphere with oxygen production effect and preparation method thereof

A core-shell structure and microsphere technology, which is applied in the field of core-shell structure microspheres with oxygen production and its preparation, can solve the problems of low oxygen release, limited oxygen production, influence on the efficiency of oxygen production, etc. The effect of slowing down decomposition, avoiding secretion, and ensuring safe use

Active Publication Date: 2021-04-02
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is only applicable to tumor cells that contain a relatively high concentration of hydrogen peroxide. For normal tissues with a very low concentration of hydrogen peroxide, the efficiency of oxygen production will be significantly and directly affected.
Moreover, the oxygen production of self-oxygen nanoparticles prepared in this patent is limited by the hydrogen peroxide content in tumor cells, which leads to the problem of low oxygen release.

Method used

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  • Core-shell structure microsphere with oxygen production effect and preparation method thereof
  • Core-shell structure microsphere with oxygen production effect and preparation method thereof
  • Core-shell structure microsphere with oxygen production effect and preparation method thereof

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Effect test

preparation example Construction

[0037] The invention provides a method for preparing core-shell structure microspheres with oxygen production, the schematic diagram of the preparation process is as follows figure 1 As shown, it specifically includes the following steps:

[0038]S1, dissolving polylactic acid-glycolic acid copolymer in an organic solvent according to a preset concentration to form an oil phase;

[0039] S2, fully mixing hydrogen peroxide and polyvinylpyrrolidone to form a complex to obtain an aqueous phase;

[0040] S3. Slowly add the water phase obtained in step S2 into the oil phase obtained in step S1, and add polyvinyl alcohol, and homogenize to obtain a primary emulsion;

[0041] S4, adding the primary emulsion obtained in step S3 into the continuous phase containing polyvinyl alcohol and hydrogen peroxide to form a secondary emulsion;

[0042] S5. After fully stirring the secondary emulsion obtained in step S4, remove the organic solvent described in step S1 by evaporation, then perfo...

Embodiment 1

[0051] This embodiment provides a method for preparing core-shell structure microspheres with oxygen generation, comprising the following steps:

[0052] S1, polylactic acid-glycolic acid copolymer (PLGA) is dissolved in methylene chloride (DCM) solution, the concentration of control PLGA is 12wt%, forms oily phase (O 1 ).

[0053] S2, hydrogen peroxide (H 2 o 2 ) mixed with polyvinylpyrrolidone (PVP) in a molar ratio of 1:7.5, stirred overnight at -4°C to form a water phase (W 1 ); among them, H 2 o 2 Thorough mixing with PVP can form a stable complex.

[0054] S3. Add 0.3 mL of the water phase obtained in step S2 dropwise to the oil phase obtained in 2 mL of step S1, then add 2 mL of polyvinyl alcohol with a concentration of 1%, as a stabilizer and emulsifier, and use a high-speed homogenizer at 10,000 rpm Homogenize 60s under the rotating speed of / min, obtain water-in-oil primary emulsion (W 1 / O 1 ).

[0055] S4, all primary emulsion that step S3 obtains is poure...

Embodiment 2～4

[0059] Embodiments 2 to 4 respectively provide a method for preparing core-shell microspheres with oxygen production. Compared with Example 1, the difference is that the PVA concentration in the continuous phase in step S4 is changed, and the remaining steps are all It is consistent with Embodiment 1 and will not be repeated here. Wherein, the PVA concentrations corresponding to Examples 2-4 are 0.6%, 0.8% and 1.2% respectively.

[0060] The oxygen production capacity of the core-shell structure microspheres with oxygen production effect prepared in Example 1 and Examples 2 to 4 was tested, specifically including the following steps:

[0061] Prepare a catalase solution with a concentration of 1% according to catalase: deionized water=1:100, then put the microspheres prepared in Examples 1 to 4 into the catalase solution, 30min, 60min and 90min test the amount of oxygen produced.

[0062] According to the above test results, the comparison diagrams of the oxygen levels produ...

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Abstract

The invention provides a core-shell structure microsphere with an oxygen production effect and a preparation method thereof. The preparation method comprises the following steps of: adding a water phase formed by complexing hydrogen peroxide and polyvinylpyrrolidone into an oil phase containing a polylactic acid-glycolic acid copolymer to form a water-in-oil primary emulsion; then adding the water-in-oil primary emulsion into a continuous phase containing polyvinyl alcohol and hydrogen peroxide to form a water-in-oil-in-water secondary emulsion; removing a solvent, and centrifuging, washing and drying to prepare the core-shell structure microsphere which takes the hydrogen peroxide as a core body and takes the polylactic acid-glycolic acid copolymer as a shell layer. With the adoption of the manner, the polyvinylpyrrolidone and the hydrogen peroxide can be stably complexed and the decomposition of the hydrogen peroxide is alleviated; the core-shell structure of the microsphere is utilized to prevent the hydrogen peroxide from being in direct contact with cells; and the safety of the cells is guaranteed and the hydrogen peroxide reacts under the catalysis of catalase in bodies to generate oxygen and water, so that the oxygen production effect is realized.

Description

technical field [0001] The invention relates to the technical field of oxygen-generating materials, in particular to a core-shell microsphere with an oxygen-generating effect and a preparation method thereof. Background technique [0002] When the skin is damaged, the tissue is rapidly hypoxic and ischemic. Under the condition of sufficient oxygen, oxygen will lead to new angiogenesis, while hypoxia will lead to apoptosis and intensified inflammatory response, and the high energy demand for inflammatory response and tissue regeneration further aggravates the problem of insufficient oxygen supply to the wound, forming vicious circle. More importantly, when the damaged tissue perceives its own hypoxia-related signals, it will release the "hypoxia-inducing factor" autonomously, which induces excessive secretion, excessive deposition and disordered arrangement of collagen, and finally leads to abnormal healing of the wound surface, forming scar. The formation of scar often re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/34A61K47/58A61K33/40A61P17/02
CPCA61K9/5031A61K47/58A61K33/40A61P17/02
Inventor 张强周水清闫书芹尤仁传李秀芳罗祖维徐安长
Owner WUHAN TEXTILE UNIV
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