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A strain of Lactobacillus reuteri capable of inhibiting the thickening of the cuticle of the skin and its application

A technology of Lactobacillus reuteri and pharmaceutical excipients, applied in the field of microorganisms, can solve problems such as long-term side effects and short-term side effects, and achieve the effect of inhibiting thickening and relieving psoriasis

Active Publication Date: 2022-04-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of tar products has side effects, including short-term side effects and long-term side effects

Method used

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  • A strain of Lactobacillus reuteri capable of inhibiting the thickening of the cuticle of the skin and its application
  • A strain of Lactobacillus reuteri capable of inhibiting the thickening of the cuticle of the skin and its application
  • A strain of Lactobacillus reuteri capable of inhibiting the thickening of the cuticle of the skin and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the cultivation of Lactobacillus reuteri and the preparation of bacterial suspension

[0045] Lactobacillus reuteri (Lactobacillus reuteri) CCFM1132 was inserted into the mMRS solid medium and cultured at 37°C for 48 hours, and its colonies were observed under a microscope. It was found that the colonies were milky white, irregular and round Raised, smooth, slightly irregular-shaped, rounded-ended campylobacters, usually present singly, in pairs, and in small clusters.

[0046] Insert Lactobacillus reuteri (Lactobacillus reuteri) CCFM1132 into MRS liquid medium and culture at 37°C for 30 hours, then transfer to fresh MRS liquid medium, and cultivate under the same conditions for 30 hours, centrifuge the bacteria at 6000×g for 15 minutes, 0.9% After washing with normal saline, the cells were centrifuged again at 6000×g for 10 min, and the cells were collected, resuspended with 30% (m / v) sucrose solution, and frozen at 80°C until use.

[0047] Optionally, w...

Embodiment 2

[0048] Example 2: Relief effect of Lactobacillus reuteri on lesion skin of psoriasis mice

[0049] The 6-8 week-old SPF BALB / c female mice were divided into 4 groups, which were the normal group, the model group and the experimental group. L. reuteri 1 group of L. reuteri1, 6 animals in each group, were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, with a constant temperature of 21-26°C, humidity of 40-70%, and noise less than or equal to 60dB. Illumination 15-20LX (all animal experiment procedures were reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0050] The experimental period was 3 weeks in total, and the model was made in the third week. The back of the mouse was depilated the day before the model was made, and the area was about 2.5cm×2.5cm. During the modeling period, imiquimod cream was applied to the ear and back depilated areas of the mice in the model group and the expe...

Embodiment 3

[0053] Example 3: Inhibitory effect of Lactobacillus reuteri on horny thickening in psoriasis mice

[0054] The 6-8 week-old SPF BALB / c female mice were divided into 4 groups, which were the normal group, the model group and the experimental group. L. reuteri 1 group of L. reuteri1, 6 animals in each group, were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, with a constant temperature of 21-26°C, humidity of 40-70%, and noise less than or equal to 60dB. Illumination 15-20LX (all animal experiment procedures were reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0055] The experimental period was 3 weeks in total, and the model was made in the third week. The back of the mouse was depilated the day before the model was made, and the area was about 2.5cm×2.5cm. During the modeling period, imiquimod cream was applied to the ear and back depilated areas of the mice in the model group and...

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Abstract

The invention discloses a strain of Lactobacillus reuteri capable of inhibiting the thickening of the cuticle of the skin and an application thereof, belonging to the technical field of microbes and the technical field of medicine. The invention provides a new application of Lactobacillus reuteri CCFM1132 or a fermentation agent containing Lactobacillus reuteri CCFM1132 in the preparation of products for alleviating psoriasis. The situation takes a turn for the better, and the thickening of skin epidermis is suppressed; IL-23 level reduces 37.6%, IL-17 level reduces 18.5% in the psoriasis sample mouse skin, in the preparation prevention and / or the product (such as food) of psoriasis treatment , medicine or health food, etc.), has a huge application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus reuteri capable of inhibiting the thickening of the cuticle of the skin and an application thereof, belonging to the technical field of microbes and the technical field of medicine. Background technique [0002] The skin is an organ that wraps the body surface and directly contacts with the external environment. It has the functions of protection, excretion, temperature regulation and external stimulation. It is the largest organ in the human body. The skin is divided into two layers, the epidermis and the dermis, and the epidermis is on the surface of the skin. The stratum corneum is the outermost part of the epidermis and consists mainly of 10 to 20 layers of flat, dead cells without nuclei. When these cells are shed, the underlying cells in the basal layer are pushed up to form a new stratum corneum. The main function of the stratum corneum is to protect its subcutaneous tissue from infection, deh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P17/06A23L33/135A23L33/00A23L11/50A23L11/45A23L11/65A23L19/20A23L2/38A23L2/39C12R1/225
CPCC12N1/20A61K35/747A61P17/06A23L33/135A23L33/00A23C11/106A23C20/025A23L19/20A23L2/382A23L2/39A23V2002/00A23V2400/173A23V2200/318
Inventor 陈卫陆文伟邓雅丹翟齐啸田丰伟崔树茂王刚赵建新张灏
Owner JIANGNAN UNIV
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