Method for detecting six trace elements in whole blood by using internal standard combined solution

An internal standard element and solution technology, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as the application of trace elements affecting the detection of blood, the excessive number of internal standard combinations, and the reduction of sensitivity, so as to reduce human operation errors, prepare Simple operation and high sensitivity

Active Publication Date: 2021-03-23
BIOYONG TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when using the same internal standard system to detect multiple trace elements, it is necessary to use various elements with similar mass numbers and ionization energies as the internal standard, resulting in too many currently available internal standard combinations, resulting in the use of inconvenient
However, if only too few internal standard quantities (such as 1-2 types) are used, although the cost can be reduced and the use is convenient, there is a problem of reduced sensitivity due to the lack of necessary internal standard elements with similar mass numbers of the elements to be measured. Insufficient, affecting the application of ICP-MS detection of trace elements in blood

Method used

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  • Method for detecting six trace elements in whole blood by using internal standard combined solution
  • Method for detecting six trace elements in whole blood by using internal standard combined solution
  • Method for detecting six trace elements in whole blood by using internal standard combined solution

Examples

Experimental program
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Effect test

Embodiment 1

[0080] Embodiment 1: Preparation of internal standard calibrator of manganese element in human whole blood

[0081] (1) 1% HNO 3 : Measure 5mL of HNO 3 , dilute to a 500mL volumetric flask with purified water, and mix well;

[0082] (2) Preparation of sample diluent: measure 0.5mL of HNO 3 , 0.5mL of Triton, 10mL of isopropanol were distilled to a 500mL volumetric flask with purified water, and mixed;

[0083] (3) Manganese internal standard calibration product preparation in human whole blood: the single element standard solution of germanium Ge and scandium Sc is diluted 100 times with 1% HNO3, the total volume is 10mL, and the concentration is 10mg / L to obtain the manganese element in human whole blood Internal standard stock solution; then take 0.4mL human whole blood manganese element internal standard stock solution and dilute it 100 times with sample diluent, the total volume is 100mL, the concentration is 40μg / L, and the human whole blood manganese element internal ...

Embodiment 2

[0084] Embodiment 2: Calibrate the trace element analyzer with internal standard calibrator

[0085] The purpose of the experiment: to calibrate and optimize the repeatability and stability of the trace element analyzer (Clin-ICP-QMS-I) by using the internal standard calibrator.

[0086] experimental method:

[0087] (1) Put the internal standard calibration product at room temperature (10-30°C), shake well and set aside.

[0088] (2) When testing, inject the internal standard calibrator and external standard calibrator (or quality control product, or unknown sample) at the same time.

[0089] (3) Adjust the parameters of the trace element analyzer (Clin-ICP-QMS-I) to the best state according to the instrument operating procedures. After the instrument is stabilized, the internal standard calibrator and the external standard calibrator are injected at the same time, and the external standard calibrator is injected sequentially (from No. 0 to No. 5). Each sample needs to cons...

Embodiment 3

[0091] Example three: detection of manganese element content in 116 cases of human whole blood samples

[0092] experimental method:

[0093] 1. Sample collection and storage

[0094] (1) The blood collection personnel should try not to wear latex gloves when collecting blood. They can use plastic gloves, or clean their hands without gloves; Wipe the iodine with alcohol); (2) Collect about 2mL of whole blood using a sodium heparin anticoagulant blood collection tube, make sure the cap is tightly closed, and immediately mix by inverting gently to avoid coagulation. If it cannot be submitted for inspection within 2 hours, it should be stored at 2-8°C for a maximum of 14 days.

[0095] 2. Sample pretreatment

[0096] Dilute 20 times: Whole blood sample (>200μL) is mixed with a blood mixer for 5-8 minutes, pipette 100μL blood sample into a centrifuge tube containing 1.9mL diluent with a calibrated pipette gun, and place in a vortex Mix well on the shaker and wait for testing o...

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Abstract

The invention discloses a method for determining six trace elements in human whole blood by using inductively coupled plasma mass spectrometry. The method comprises the following steps of: collectingand treating a blood sample; adding a quality control product for quality control, and performing instrument detection; and drawing a calibration curve and calculating the blood content of trace elements according to a regression equation. According to the internal standard combined solution for detecting the six single elements in the whole blood, the six trace elements are magnesium, aluminum, calcium, vanadium, chromium and manganese, and the internal standard combined solution is selected from an internal standard combined solution of scandium (Sc) and germanium (Ge). The method is high insensitivity, simple in pretreatment, short in detection time, accurate in result and good in stability, and has a wide application prospect in the field of medicine and health.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to an internal standard calibrator for measuring Mn element in human whole blood by using inductively coupled plasma mass spectrometry, and a method for measuring Mn element in human whole blood by using inductively coupled plasma mass spectrometry. Background technique [0002] According to the content of elements in the human body, the elements in the human body can be divided into macro elements and trace elements. The macro elements refer to the elements that account for more than one ten thousandth of the body weight, including calcium (Ca), sodium (Na), phosphorus (P), Potassium (K), Magnesium (Mg), Chlorine (Cl) and Sulfur (S), etc., while trace elements refer to content below one ten-thousandth of the total amount of the human body, and the daily intake is low Elements in 100mg. In 1995, copper (Cu), cobalt (Co), chromium (Cr), iron (Fe), fluorine (F), iodine (I), manganes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/626
CPCG01N27/626
Inventor 马庆伟朱柯文熊婵向华
Owner BIOYONG TECH
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