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Application of fungal microbiome as marker in preparation, treatment, screening and lung cancer diagnosis

A lung cancer diagnosis and microbial technology, applied in the direction of microbial-based methods, biochemical equipment and methods, microbial measurement/testing, etc., to achieve the effect of accurate screening and auxiliary diagnosis

Active Publication Date: 2021-03-23
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008]D. At present, the sample size of lung cancer microbiome research is small, and multi-center cooperation is needed to obtain large sample data with statistical significance;

Method used

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  • Application of fungal microbiome as marker in preparation, treatment, screening and lung cancer diagnosis
  • Application of fungal microbiome as marker in preparation, treatment, screening and lung cancer diagnosis
  • Application of fungal microbiome as marker in preparation, treatment, screening and lung cancer diagnosis

Examples

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Embodiment 1

[0105] ITS sequencing results of cancer tissues and paracancerous tissues of lung adenocarcinoma patients:

[0106] In order to analyze the fungal microbiome structure of lung adenocarcinoma patients, this example firstly sequenced the cancer tissue (tumor tissue) and paracancerous tissue derived from lung adenocarcinoma patients. The results showed that the Shannon index of cancer tissue and paracancerous tissue had no difference significantly different (Fig. 1a), and the abundance of paracancerous tissues was significantly higher than that of cancerous tissues ( Figure 1b ). The results of principal component analysis further verified that cancer tissue and paracancerous tissue contained significantly different fungal flora ( Figure 1c ), the ratio of Basidiomycetes / Ascomycetes in paracancerous tissue was similar to that of PG1 lung cancer tissue, but significantly lower than that of PG2 lung cancer tissue, and the ratio in PG1 and PG2 was significantly higher than that o...

Embodiment 2

[0108] In order to deeply analyze the fungal microbiome structure in different parts of lung cancer patients and identify the specific Aspergillus species, cancer tissues, paracancerous tissues, alveolar lavage fluid and stool samples were collected from 20 untreated patients with lung adenocarcinoma. By extracting microbiome DNA and performing metagenomic sequencing ( Figure 2a ), it was found that lung tissue and BAL of patients with lung adenocarcinoma had higher Shannon index and flora abundance than the intestinal fungal microbiome ( Figure 2b ). Then the correlation between the lung and intestinal fungal microbiome was verified, and PCA results analysis showed that the lung and intestinal flora clustered independently, with obvious characteristics ( Figure 2c ). A reduced alpha diversity and a different proportional community composition of fungi were further observed in the lung compared to the gut ( Figure 2c-2d ). Pneumocystis jiroveci was widely present in tu...

Embodiment 3

[0110] In order to study whether there are differences in the fungal flora in different locations of the lung, the microbiota of tumor tissue, paracancerous tissue, and bilateral BAL (i.e., tumor-side alveolar lavage fluid and non-tumor side alveolar lavage fluid) from LUAD patients were analyzed. Horizontal analysis of changes in fungal microbiome structure in lung adenocarcinoma patient tissues and lavage fluids. Such as Figure 4a As shown, the fungi / bacteria ratio was higher in tumor tissues than in tumor-side BAL, that is, fungi were more enriched in tumor tissues than in BAL. In both BAL and tumor tissues, the fungal flora was dominated by Ascomycetes and Basidiomycetes ( Figure 5 ). Furthermore, the ratio of Basidiomycota / Ascomycota was higher in BAL than in tissues ( Figure 4b ). α-diversity analysis showed that there were significant differences in the Shannon index and richness of the fungal flora between BAL and lung tissues in patients' lung tissues, and ther...

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Abstract

The invention provides application of a fungal microbiome as a marker in preparation, treatment, screening and lung cancer diagnosis. By constructing a fungal microbiome DNA enrichment and extractionmethod, fungal microbiome DNA is extracted from tissues, feces and alveolar lavage fluid, and ITS sequencing and metagenome sequencing are applied to fungal microbiome and gene function analysis, wherein aspergillus polyangius is enriched in tumor tissues, candida albicans is enriched in tumor tissues and para-carcinoma tissues, and malassezia is enriched in alveolar lavage fluid; by detecting these target strains or molecular markers, lung cancer can be found and intervened as early as possible, and the target strains or molecular markers are expected to be used as important indicators for lung cancer screening; and in addition, the characteristics of the type and quantity of fungi are obtained through DNA sequencing results of fungal florae in feces, tissues and alveolar lavage fluid samples of different parts of lung adenocarcinoma patients, and the lung cancer is more accurately screened and diagnosed in an assisted mode based on the quantity and ratio characteristics of the fungispecifically enriched in the lung cancer.

Description

technical field [0001] The invention belongs to the technical field of microbiome or lung cancer, and specifically relates to the application of a fungal microbiome as a marker in the preparation of treatment screening and lung cancer diagnosis. Background technique [0002] Current research on the molecular basis of cancer development has focused on changes in the sequence or structure of the human genome, but recent studies have shown strong associations between different cancer types and specific microbiomes; however, the extent of this association and its variation, and its The impact on cancer diagnosis, prognosis, and treatment is unclear. Various parts of the human body have different microbial flora composition, and the flora flora varies greatly among individuals, and changes dynamically with the health status and age of the human body. The stability of the microbial flora is very important for human health and immune status. Dysregulation may affect the body's imm...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/06C12Q1/04C12R1/66C12R1/725C12R1/645
CPCC12Q1/6895C12Q1/06C12Q1/04G01N2800/7028
Inventor 王慧刘宁宁
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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