Two Active Peptides with Bitter Taste Inhibition

A technology of inhibitory and active peptides, applied in the field of active peptides with bitterness inhibitory effects, can solve the problems of large loss of peptides, affecting the nutrition and activity of peptides, and additional ingredients that do not conform to new dietary concepts, etc., to achieve wide application prospects, water-soluble sex good effect

Active Publication Date: 2022-04-22
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the selective separation method has a relatively large loss on the polypeptide, which affects the nutrition and activity of the polypeptide, especially for substances with bitter peptides as the main active component; the masking method needs to add bitter taste such as cyclodextrin and modified starch. Masking agents or other flavoring substances, the additional ingredients of which do not conform to the new concept of healthy and natural diet

Method used

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  • Two Active Peptides with Bitter Taste Inhibition
  • Two Active Peptides with Bitter Taste Inhibition
  • Two Active Peptides with Bitter Taste Inhibition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Screening of Peptides with Bitter Taste Inhibitory Activity

[0028]The present invention uses ExPASy PeptideCutter (http: / / web.expasy.org / peptide_cutter / ), an online enzyme cutting tool, to perform virtual enzyme cutting on the rainbow trout concomitant actin sequence to obtain 687 dipeptides and above active peptides. ADMET procedure via online tools peptide property calculator (http: / / www.innovagen.com / proteomics-tools), PeptideRanker (http: / / distilldeep.ucd.ie / PeptideRanker / ) and Discovery Studio (DS) 2017 R2 Client software Water solubility, biological activity and toxicity were predicted for 687 active peptides, and 22 peptides GKP, LPPDAPEL, PASDNPVL, DW, SM, DM, DAM, GSG, NDPQF, CSPVDM, PDSM, ADW, DGM, PADAPEF, PADSPQF, SDW, SPVDM, MPDSM, ADM, IPPDM, SIPDRPEF, and PDIM. The crystal structure of the bitter taste receptor T2R14 was obtained from the BitterDB database (http: / / bitterdb.agri.huji.ac.il / dbbitter.php) and used as a protein target. Molecula...

Embodiment 2

[0030] Example 2. Determination of the inhibitory activity of active peptides to bitter substances

[0031] The bitter taste inhibitory activity of certain concentrations of peptides ADM, ADW, SPVDM and CSPVDM was determined by electronic tongue. A certain amount of peptides ADM, ADW, SPVDM and CSPVDM were taken respectively, added to 30mL of water and mixed evenly, and 50mL of quinine (1mM) was added to prepare the solution to be tested. Quinine was used as the blank control group, and peptide LEGSLE was used as the positive control group. After setting the number of cycles, data file name, sample quantity, sample name and test method, install the electrode and start the measurement.

[0032] The measurement process is as follows: (1) Clean the sensor in the positive and negative solutions for 90s, and then clean the sensor in the two reference solutions for 120s; (2) Balance the sensor in the conditioning solution for 30s; (3) Measure each sample for 30s; (4 ) After cleanin...

Embodiment 3

[0034] Example 3. Application of Bitter Taste Inhibiting Active Peptide ADM, ADW

[0035] In actual production, bitter taste inhibitory active peptides ADM and ADW can be prepared by solid-phase chemical synthesis, and added to bitter foods, medicines and health products in the form of powder, granule or water-soluble liquid. For example, active peptides are added to tea and coffee to suppress the bitterness in the drinks while retaining the unique flavor in the drinks; active peptides are added to bitter melon to suppress its bitterness to make dried bitter melon, bitter melon tea and other bitter melon processing products, eliminate people’s resistance to bitter gourd, improve the edible and medicinal value of bitter gourd for clearing away heat and detoxification, nourishing blood and nourishing liver, and lowering blood sugar; The bitterness of the ingredients is suppressed, which helps people take the medicine more happily; adding active peptides to health foods such as d...

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Abstract

The present invention discloses two active peptides with bitter taste inhibitory effect, the amino acid sequences of which are Ala-Asp-Met and Ala-Asp-Trp respectively. The two active peptides with bitter taste inhibitory effect provided by the present invention can effectively combine with the bitter taste receptor T2R14, have a sustained and stable inhibitory effect on bitter substances, and have the characteristics of safety, non-toxic side effects, good water solubility, etc., and can be used for It has broad application prospects and very important significance in food, medicine and health care products.

Description

technical field [0001] The invention belongs to the field of bioactive peptides, and in particular relates to two active peptides with bitter taste inhibitory effect. Background technique [0002] Humans are able to perceive five basic tastes, namely sour, sweet, bitter, salty and umami. Bitterness is considered an unpleasant taste sensation that can be formed during food proteolysis and pharmaceutical processing. Suppressing bitterness is important, a long-standing challenge in the food and pharmaceutical industries, since bitterness can negatively affect food intake and the bitterness of certain drugs makes it difficult for patients to swallow. [0003] Bitter taste perception is mediated by bitter taste receptors TAS2Rs, which are expressed on the human tongue and belong to the G protein-coupled receptor family. To date, almost all 25 human bitter taste receptors have been identified, but most TAS2Rs still have no known ligands. These TAS2Rs receptors possess a single ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/083C07K14/46A23L29/00A23L5/20A61K47/18
CPCC07K5/0806C07K14/461A23L29/055A23L29/045A23L5/27A61K47/183
Inventor 于志鹏王莹雪赵文竹励建荣
Owner BOHAI UNIV
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