Fluorescence spectrum detection method based on smart phone

Abstract

The invention discloses a fluorescence spectrum detection method based on a smart phone, belonging to spectrum detection and image processing. The method comprises the following steps: 1) fluorescencesignal detection: carrying out fluorescence signal detection by using a camera of a smart phone as a detector; 2) image data processing: processing the video data shot by the smart phone; and 3) drawing of a spectral curve: selecting a proper color value as a fluorescence intensity parameter of a sample according to the color channel information of the effective light-emitting area of a sample fluorescence image read in the step 2), drawing the characteristic fluorescence spectral curve of the sample by taking wavelength as an abscissa and fluorescence intensity as an ordinate, customizing the name of the characteristic fluorescence spectrum curve, storing the name in a specified folder, and generating a fluorescence substance spectrum database. According to the invention, the fluorescence spectrum of a substance can be quickly and accurately scanned in a video recording form through an image sensor of the camera of the smart phone; and operation is simple, analysis time is short, anddetection sensitivity can be improved by reducing the interference of environmental noise.

Description

technical field [0001] The invention relates to the technical field of spectrum detection and image processing, in particular to a smart phone-based fluorescence spectrum detection method. Background technique [0002] Fluorescence analysis has the advantages of high sensitivity, strong selectivity and various methods, and has been widely used in the fields of chemistry, biology, medicine, health, agriculture and environmental protection. As a characterization technique, fluorescence spectroscopy is used to study the physical and chemical properties and changes of the system, and can be used as a means of qualitative detection and quantitative analysis. The operation of the traditional fluorescence analysis method is relatively complicated, which is not conducive to popularization, and the instrument is large in size and high in manufacturing cost, making it difficult to apply to on-site detection. Therefore, the research on new rapid fluorescence analysis technology and po...

AI Technical Summary

Problems solved by technology

[0004] The photodetectors used in traditional fluorescence analysis instruments are mainly PMTs (photomultiplier tubes), which need to provide a stable negative high voltage device, making it difficult to miniaturize the instrument

At present, the devices used in portable spectral detection methods based on smartphones often require additional optical components, the optical path design is relatively complicated, and the cost is high

At the same time, the existing spectral detection method based on smartphones acquires the spectrum by taking a single sample picture, which has shortcomings such as small detection range, single detection object, complex image processing, and long analysis time.

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