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Method for preparing silk fibroin base bi-metal antibacterial coating

A silk fibroin, antibacterial coating technology, applied in coating, surgery, medical science and other directions, can solve the problem of inability to deal with bacterial infection environment, biological toxicity, single antibacterial agent, etc., and achieve long-term high-efficiency bactericidal ability and uniform particle size , Long-term high-efficiency sterilization effect

Active Publication Date: 2021-03-09
NORTHWEST INSTITUTE FOR NON-FERROUS METAL RESEARCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The disadvantage of the existing antibacterial coating technology is that most of the current drug-loaded coatings contain a single antibacterial agent, which cannot cope with bacterial infection environments with higher bacterial concentrations or stronger bacterial activity, and high concentrations of antibacterial substances nano-silver and nano-gold will Potential biological toxicity, how to achieve long-term and high-efficiency antibacterial effect at low concentration and dosage has become a challenge

Method used

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  • Method for preparing silk fibroin base bi-metal antibacterial coating
  • Method for preparing silk fibroin base bi-metal antibacterial coating
  • Method for preparing silk fibroin base bi-metal antibacterial coating

Examples

Experimental program
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Effect test

Embodiment 1

[0037] This embodiment includes the following steps:

[0038] Step 1. Place the silk in 0.02M Na 2 CO 3 Boil in the solution for 40 minutes, take it out, rinse it with deionized water, and then add it to 9.3M LiBr solution at 60°C for 4 hours to obtain the silk fibroin stock solution;

[0039] The silk fibroin stock solution is diluted with deionized water into a silk fibroin solution with a mass concentration of 2%, then 17mg of silver nitrate powder and 34mg of tetrachloroauric acid powder are added to the silk fibroin solution of 100mL with a mass concentration of 2%, and the Stir while adding to obtain a mixed solution A containing silk fibroin, silver ions and gold ions and having a pH of 8.5; the concentration of silver ions in the mixed solution A is 1 mM, and the concentration of gold ions is 1 mM;

[0040] Step 2. Place the mixed solution obtained in step 1 under a 40W ultraviolet lamp for ultraviolet irradiation for 0.5h, so that the color of the mixed solution A g...

Embodiment 2

[0066] This embodiment includes the following steps:

[0067] Step 1. Place the silk in 0.02M Na 2 CO 3 Boil in the solution for 40 minutes, take it out, rinse it with deionized water, and then add it to 9.3M LiBr solution at 60°C for 4 hours to obtain the silk fibroin stock solution;

[0068] The silk fibroin stock solution is diluted into the silk fibroin solution of mass concentration 4% with deionized water, then 17mg silver nitrate powder and 34mg tetrachloroauric acid powder are added in the silk fibroin solution of 100mL mass concentration 2%, and Stir while adding to obtain a mixed solution A containing silk fibroin, silver ions and gold ions and having a pH of 8.5; the concentration of silver ions in the mixed solution A is 10mM, and the concentration of gold ions is 10mM;

[0069] Step 2. Place the mixed solution obtained in step 1 under a 40W ultraviolet lamp for ultraviolet irradiation for 1 hour, so that the color of the mixed solution A gradually changes from mil...

Embodiment 3

[0073] This embodiment includes the following steps:

[0074] Step 1. Place the silk in 0.02M Na 2 CO 3 Boil in the solution for 40 minutes, take it out, rinse it with deionized water, and then add it to 9.3M LiBr solution at 60°C for 4 hours to obtain the silk fibroin stock solution;

[0075] The silk fibroin stock solution is diluted with deionized water into a silk fibroin solution with a mass concentration of 5%, then 17mg of silver nitrate powder and 34mg of tetrachloroauric acid powder are added to the silk fibroin solution of 100mL with a mass concentration of 2%. Add while stirring to obtain a mixed solution A containing silk fibroin, silver ions and gold ions and having a pH of 8.5; the concentration of silver ions in the mixed solution A is 20mM, and the concentration of gold ions is 20mM;

[0076] Step 2. Place the mixed solution obtained in step 1 under a 40W ultraviolet lamp for ultraviolet irradiation for 2 hours, so that the color of the mixed solution A gradu...

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Abstract

The invention discloses a method for preparing a silk fibroin base bi-metal antibacterial coating. The method comprises the following steps of: 1: adding silver nitrate powder and chloroauric acid powder into a silk fibroin solution to obtain a mixed solution A; 2: carrying out ultraviolet radiation on the mixed solution to obtain a mixed solution B; 3: dipping metal in a working solution to carryout light-avoidance dipping to obtain the metal of which the surface is provided with a polydopamine assistance layer; and 4: dipping the metal of which the surface is provided with the polydopamineassistance layer into the mixed solution B to form the silk fibroin base bi-metal antibacterial coating on the surface of the metal. Through the ultraviolet radiation, silk fibroin is subjected to in-situ reduction, the concentration of nano-silver particles and gold nanoparticles is lowered, meanwhile, since the polydopamine assistance layer is introduced into the surface of a metal substrate, the binding performance of the metal and the silk fibroin base bi-metal antibacterial coating is greatly enhanced, a long-term efficient synergetic sterilization function is realized, the problem of cell infection of an implant is effectively solved, and potential biotoxicity is reduced.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a method for preparing a silk fibroin-based bimetallic antibacterial coating. Background technique [0002] Medical pure titanium has been widely used in the field of clinical medicine because of its excellent biocompatibility. As early as the 1950s, pure titanium was processed into bone plates, bone nails, femoral heads and other implanted medical devices for orthopedic repair operations. Subsequently, pure titanium has been continuously applied clinically, and now it is mainly used in the field of oral restoration, such as: crowns and bridges (inlays, cores, crowns, bridges), brackets (partial denture brackets, complete denture bases), various attachments, and prosthetics. [0003] However, as a common postoperative complication, bacterial infection caused by medical devices has become one of the important problems to be solved in the field of medicine in the 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/02A61L31/08A61L31/10A61L31/14A61L31/16
CPCA61L31/022A61L31/10A61L31/088A61L31/14A61L31/16A61L2300/102A61L2300/104A61L2300/404A61L2300/602A61L2300/606A61L2420/02A61L2420/08C08L79/02C08L89/00
Inventor 周文昊余森荆磊张亚峰汶斌斌
Owner NORTHWEST INSTITUTE FOR NON-FERROUS METAL RESEARCH
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