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Dengue virus high-sensitivity detection method established based on nano magnetic bead immunochromatography technology

A technology of nano-magnetic beads and dengue virus, which is applied in the direction of measuring devices, analytical materials, and biological material analysis, and can solve the problems of low sensitivity, unfavorable large-scale detection, and high cost

Active Publication Date: 2021-03-05
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the virus isolation operation is cumbersome and has low sensitivity, which is not suitable for early diagnosis; the RT-PCR method requires professional equipment, the process is complicated, the cost is high, and it is not conducive to large-scale detection; the ELISA method is complicated to operate, and its sensitivity and specificity are lower than RT. -PCR method; colloidal gold method is slightly lower in sensitivity and specificity than ELISA method, and the cost is higher than ELISA method

Method used

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  • Dengue virus high-sensitivity detection method established based on nano magnetic bead immunochromatography technology
  • Dengue virus high-sensitivity detection method established based on nano magnetic bead immunochromatography technology
  • Dengue virus high-sensitivity detection method established based on nano magnetic bead immunochromatography technology

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Embodiment 1

[0073] 1 Materials, methods and technical route

[0074] 1.1 virus

[0075] Dengue virus recombinant protein NS1 (E.coli), Zika virus (ZIKV), influenza virus and serum samples used in this study were provided by Beijing Dahong Biotechnology Co., Ltd.

[0076] 1.2 Materials and Instruments

[0077] Sodium acetate, FeCl 3 ·6H 2 O, ethanol, ethylene glycol (Beijing Chemical Reagent Factory), 10mg / mLN-

[0078] (3-Dimethylaminopropyl)-N'-ethylcarbodiimide. Hydrochloride (EDC), 10mg / ml N-Hydroxysulfosuccinimide. Sodium salt (NHS), 0.1M Tris , 5% BSA, 1% BSA, 10mM PB, DAB chromogenic solution (Zhongshan Jinqiao), magnetic beads (Jinzhun Zhizao Biotechnology Company, particle size 50nm), transmission electron microscope (JEOL2000FX 20\0KV, Japan Electronics Corporation), High temperature oven (Tianjin Tester), enzyme-linked immunosorbent analyzer (Bio-Rad), PVDF membrane (Millipore), cut-flow spray film marking machine (Arista, USA), strip cutting machine (Tianjin Keju Bio), XYZ...

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Abstract

The invention discloses a dengue virus high-sensitivity detection method established based on a nano magnetic bead immunochromatography technology. The method comprises the following steps: preparinga dengue virus nano magnetic bead-antibody probe; and assembling a nano magnetic bead test paper strip. According to the invention, the novel dengue virus detection method based on the nano magnetic bead immunochromatography technology is established by using the nano magnetic bead test paper strip to detect the dengue virus and utilizing the magnetic enrichment and separation functions of the nano magnetic beads and the characteristic that color signals can be observed by naked eyes, so that the detection sensitivity can be effectively improved, and the method is simple, convenient and easy to operate, and can be used for large-scale rapid diagnosis.

Description

technical field [0001] The invention relates to the technical field of nano magnetic bead immunochromatography. Specifically, a high-sensitivity detection method for dengue virus is established based on nano-magnetic bead immunochromatography technology. Background technique [0002] Dengue fever (Dengue Fever) is an acute infectious disease caused by Dengue Virus (DENV) of the flavivirus genus. Dengue virus is transmitted by vector insects such as Aedes aegypti and Aedes albopictus to cause dengue fever, which is widespread in tropical and subtropical regions of the world. Mild symptoms are persistent fever, headache, joint pain, muscle pain, etc., and severe cases have a high mortality rate. High dengue hemorrhagic fever and dengue shock syndrome. Due to factors such as global warming and reckless urban sprawl, the global incidence of this remarkably fast-spreading viral disease has increased 30-fold over the past 50 years. According to statistics, 390 million people ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/558G01N33/54346G01N33/54326G01N2333/185
Inventor 刘键刘昕张永江
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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