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Bacillus tequilensis D5-8 and application thereof

A technology of Bacillus tequila and Bacillus, which is applied in the field of agricultural microorganisms, can solve the problem of few bacteria involved in biocontrol, and achieve the effect of low cost, good effect and good inhibitory effect

Active Publication Date: 2021-02-23
YUNNAN TOBACCO CO LTD KUNMING BRANCH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Correspondingly, the research and development of microbial pesticides for tobacco black shank has become a major direction in the field of tobacco black shank disease control in recent years, but at present, the biocontrol bacteria of black shank are mainly screened from plants and rhizosphere soil, but other sources Biocontrol involves little

Method used

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  • Bacillus tequilensis D5-8 and application thereof
  • Bacillus tequilensis D5-8 and application thereof
  • Bacillus tequilensis D5-8 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation, screening and fermentation of embodiment 1 Tequila bacillus D5-8

[0027] 1. Separation and screening

[0028] Periplaneta americana is a strain raised in the Laboratory of Vector Organisms and Insect-borne Diseases of Shandong First Medical University. It has been continuously bred in the laboratory for 15 years. When isolating Bacillus tequilensis, the body surface of the adult Periplaneta americana was rinsed 3 times with 75% alcohol for 2 minutes each time, and then rinsed 3 times with sterile water before dissecting to obtain a complete digestion of the American cockroach. road.

[0029] 1. Sterilization: All experimental equipment such as petri dishes, centrifuge tubes, test tubes, pipette tips and sterile water are sterilized by high pressure at 0.1MPa, 121°C for 30 minutes.

[0030] 2. Grinding: Cut the digestive tract of Periplaneta americana into pieces, put it into a sterile centrifuge tube, add 1ml of sterile water, and grind it thoroughly with ...

Embodiment 2

[0062] Embodiment 2 Tequila bacillus D5-8 fermented liquid is to the inhibitory effect of tobacco Phytophthora pathogenic bacteria

[0063] The result of confrontation culture experiment on OA plate showed that the inhibition rate of D5-8 to Phytophthora nicotianae was 67%.

[0064] Table 1

[0065]

Embodiment 3

[0066] Example 3 Tequila bacillus D5-8 fermented liquid is to indoor control effect of Phytophthora tabacum

[0067] The D5-8 fermented liquid can be used for field root irrigation, and the average control effect on tobacco Phytophthora can reach 67%.

[0068] Table 2 Control effect of D5-8 on black shank

[0069]

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Abstract

The invention relates to Bacillus tequilensis D5-8 and application thereof, and belongs to the technical field of agricultural microorganisms. The strain number of the Bacillus tequilensis is D5-8, the preservation number is CGMCC No. 15973, and the Bacillus tequilensis is classified and named as Bacillus tequilensis. The Bacillus tequilensis D5-8 fermentation liquor D5-8 has a good inhibiting effect on Phytophthora nicotianae, can be used as a dosage form of a living microbial agent and a metabolite for processing and development, is used for preventing and controlling the Phytophthora nicotianae, and is good in effect and low in cost.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and in particular relates to a strain of Bacillus tequila D5-8 and its application in preventing and treating tobacco phytophthora. Background technique [0002] Tobacco is an economic plant. In the main tobacco growing areas in my country, due to the long-term and unreasonable application of chemical agents and planting methods, the diseases on tobacco have become more and more serious, which has seriously affected the quality of tobacco leaves. Recently, the annual loss caused by Reaching hundreds of millions of dollars, the prevention and treatment is facing a huge challenge. Tobacco black shank is a very common disease in the process of tobacco planting. It occurs in all major tobacco planting areas in my country, and even highly resistant diseased plants appear in some areas. It has become a major disease that threatens tobacco production. The effective control measures for this...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22C12R2001/07C12N1/205
Inventor 王志江田泽华谢永辉詹莜国何明川吴国星张忠戴恩李明波朱桂林
Owner YUNNAN TOBACCO CO LTD KUNMING BRANCH
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