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Bacillus subtilis MC4-2 and application thereof

A technology of Bacillus subtilis and fungi, which is applied in the field of agricultural microorganisms, can solve the problems of few bio-control bacteria, and achieve low cost, good inhibitory effect and good effect

Pending Publication Date: 2021-02-12
YUNNAN TOBACCO CO LTD KUNMING BRANCH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Correspondingly, the research and development of microbial pesticides for tobacco black shank has become a major direction in the field of tobacco black shank disease control in recent years, but at present, the biocontrol bacteria of black shank are mainly screened from plants and rhizosphere soil, but other sources Biocontrol involves little

Method used

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  • Bacillus subtilis MC4-2 and application thereof
  • Bacillus subtilis MC4-2 and application thereof
  • Bacillus subtilis MC4-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation, screening and fermentation of Bacillus subtilis MC4-2

[0027] 1. Separation and screening

[0028] Periplaneta americana is a strain raised in the Laboratory of Vector Organisms and Insect-borne Diseases of Shandong First Medical University. It has been continuously bred in the laboratory for 15 years. Bacillus subtilis Bacillus subutilis At the same time, the adult surface of the American cockroach was first rinsed with 75% alcohol for 3 times, each time for 2 minutes, and then rinsed with sterile water for 3 times, and then dissected to obtain a complete digestive tract of the American cockroach.

[0029] 1. Sterilization: The petri dishes, centrifuge tubes, test tubes, pipette tips and other experimental equipment and sterile water are all sterilized by high pressure at 0.1MPa, 121°C, and sterilized for 30 minutes.

[0030] 2. Grinding: Cut the digestive tract of Periplaneta americana into pieces, put it into a sterile centrifuge tube, add 1ml...

Embodiment 2

[0064] Example 2 The inhibitory effect of Bacillus subtilis MC4-2 fermented liquid on Phytophthora tabacum pathogenic bacteria

[0065] The results of OA plate confrontation culture experiments showed that MC4-2 was effective against Phytophthora tabacum Phytophthora nicotianae The inhibition rate was 64%.

[0066] Table 1

[0067] strain Phytophthora nicota Phytophthora nicotianae Inhibition rate(%)

Embodiment 3

[0068] Example 3 Bacillus subtilis MC4-2 fermented liquid is to indoor control effect of Phytophthora tabacum

[0069] The MC4-2 fermented liquid can be used to irrigate roots in the field, and the average control effect on tobacco Phytophthora can reach 64%.

[0070] Table 2 Control effect of MC4-2 on black shank

[0071] strain Disease index Average control effect (%) MC4-2 29.63±3.70 63.86±4.64 antivirus alum 18.51±3.71 77.36±4.93 ck 82.01±1.84 -

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Abstract

The invention relates to bacillus subtilis MC4-2 and application thereof, belongs to the technical field of agricultural microorganisms, and provides bacillus subtilis with the strain number of MC4-2and the preservation number of CGMCC (No.15975, and the bacillus subtilis MC42 is named as bacillus subtilis in a classified manner. The bacillus subtilis MC4-2 fermentation broth MC4-2 has a good inhibition effect on Phytophthora nicotianae, can be used as a living microbial agent and a metabolite dosage form for processing and development, is used for prevention and control of Phytophthora nicotianae, and is good in effect and low in cost.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a bacillus subtilis MC4-2 and its application in preventing and treating tobacco phytophthora. Background technique [0002] Tobacco is an economic plant. In the main tobacco growing areas in my country, due to the long-term and unreasonable application of chemical agents and planting methods, the diseases on tobacco have become more and more serious, which has seriously affected the quality of tobacco leaves. Recently, the annual loss caused by Reaching hundreds of millions of dollars, the prevention and treatment is facing a huge challenge. Tobacco black shank is a very common disease in the process of tobacco planting. It occurs in all major tobacco planting areas in my country, and even highly resistant diseased plants appear in some areas. It has become a major disease that threatens tobacco production. The effective control measures for this k...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/125
CPCC12N1/20A01N63/22C12R2001/125C12N1/205
Inventor 田泽华王志江谢永辉詹莜国吴国星张忠戴恩李明波何明川朱桂林
Owner YUNNAN TOBACCO CO LTD KUNMING BRANCH
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