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Application of iron-containing substance to preparation of product for inhibiting dengue virus propagation

A technology of dengue fever virus and substances, applied in the field of application of iron-containing substances in the preparation of products for inhibiting the spread of dengue fever viruses

Inactive Publication Date: 2021-02-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Iron supplementation is a medical method that is generally applicable to iron-deficient and low-iron populations, but so far there is no relevant report that iron supplementation can effectively reduce the susceptibility of Aedes aegypti to dengue virus

Method used

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  • Application of iron-containing substance to preparation of product for inhibiting dengue virus propagation
  • Application of iron-containing substance to preparation of product for inhibiting dengue virus propagation
  • Application of iron-containing substance to preparation of product for inhibiting dengue virus propagation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, feeding ammonium ferric citrate to suppress the susceptibility of dengue fever virus in Aedes aegypti by feeding the extracorporeal membrane feeding system

[0030] Feeding ferric ammonium citrate in an extracorporeal membrane feeding system can reduce the infection rate of Aedes aegypti to dengue virus. The specific experiments are as follows:

[0031] 1. Divide Aedes aegypti into 4 groups, namely Mock group, 25 μM FAC group, 50 μM FAC group and 150 μM FAC group, and perform the following treatments respectively:

[0032] Mock group: each Aedes aegypti was fed a mixture of human anticoagulated whole blood samples and dengue virus type 2 (DENV-2) supernatant (1:1 volume ratio) through an extracorporeal membrane feeding system;

[0033] 25 μM FAC group: each Aedes aegypti was fed human anticoagulated whole blood samples containing ferric ammonium citrate (FAC) and dengue virus type 2 (DENV-2) supernatant (1:1 volume ratio) through an extracorporeal membrane...

Embodiment 2

[0044] Example 2. The iron-deficiency mouse model significantly increases the susceptibility of dengue virus in Aedes aegypti

[0045] Virus particles and a large amount of blood in the host body enter the Aedes aegypti body during the blood-sucking process of Aedes aegypti. Therefore, the present invention adopts the following method to carry out the infection rate test of the dengue fever virus in the Aedes aegypti body by the iron-deficiency mouse model:

[0046] 1. Preparation of normal control mice and iron-deficient AG6 mice

[0047] The four-week-old AG6 mice, weighing 15g, were randomly divided into two groups, namely the Mock group and the Iron-deficient mice group, with 4 mice in each group, and were treated as follows:

[0048] Mock group: four-week-old AG6 mice were fed normal diet for five weeks;

[0049] Iron-deficient mice group: four-week-old AG6 mice were fed with iron-deficient diet for five weeks.

[0050] Two, after two groups of mice were fed for five we...

Embodiment 3

[0062] Example 3. Iron supplementation to infected hosts can significantly inhibit the susceptibility of dengue virus in Aedes aegypti

[0063] In the Aedes aegypti blood-sucking process, virus particles and a large amount of blood in the host body enter the Aedes aegypti body, therefore, the present invention adopts the following method to carry out the infection rate test of dengue virus in the Aedes aegypti body after iron supplementation to the infected host:

[0064] 1. Preparation of iron-deficient AG6 mice

[0065] Four-week-old AG6 mice were fed with iron-deficient feed for five weeks to obtain nine-week-old iron-deficient AG6 mice, and the serum iron of mice fed for the third, fourth and fifth weeks was determined, and the results were as follows: image 3 As shown, compared with normal mice (indicated by "Mock" in the figure), the serum iron concentration of the mice was significantly reduced after feeding the iron-deficient diet for five weeks, indicating that the i...

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Abstract

The invention discloses application of an iron-containing substance to preparation of a product for inhibiting dengue virus propagation. The invention firstly discloses the application of the iron-containing substance to preparation of a product for inhibiting dengue virus propagation and / or inhibiting susceptibility of mosquitoes to dengue viruses. The invention further discloses a method for inhibiting dengue virus preparation and / or inhibiting susceptibility of the mosquitoes to the dengue viruses. According to the invention, experiments prove that hosts in different iron states, who are infected by the dengue viruses and bitten by aedes aegypti, have remarkably different virus infection rates, and the susceptibility of the aedes aegypti to the dengue viruses is remarkably negatively correlated with the iron state of the host blood. Further, the susceptibility of the aedes aegypti to the dengue viruses can be effectively reduced by supplementing iron to an iron-deficiency mouse model, which shows that the iron-containing substance can reduce the virus carrying capacity of the aedes aegypti and block propagation of the viruses in nature.

Description

technical field [0001] The invention relates to the application of iron-containing substances in the preparation of products for inhibiting dengue fever virus transmission. Background technique [0002] Aedes aegypti is a kind of insect that acquires and transmits viruses by sucking blood, and is the culprit of "dengue fever". "Dengue fever" is an acute infectious disease mediated by mosquitoes that is prevalent in tropical and subtropical regions. It has the characteristics of rapid transmission and high incidence. It has caused hundreds of millions of infections and hundreds of thousands of deaths, causing serious harm to world public health. [0003] Iron supplementation is a medical method that is generally applicable to iron-deficient and low-iron populations, but so far there is no relevant report that iron supplementation can effectively reduce the susceptibility of Aedes aegypti mosquitoes to dengue virus. Contents of the invention [0004] The technical problem ...

Claims

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Application Information

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IPC IPC(8): A61K33/26A61K31/295A61P31/14A23L33/16A23L33/10
CPCA23V2002/00A61K33/26A23L33/10A23L33/16A61P3/02A61P31/14A23V2200/30A61K31/295Y02A50/30
Inventor 程功朱毅斌
Owner TSINGHUA UNIV
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