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A method for quickly breaking dormancy of lily seed bulbs

A technology for lily seeds and bulbs, which is applied in the directions of seed and rhizome treatment, agriculture, and application, can solve the problems of lily tissue culture seed bulbs, etc., and achieve the effects of shortening dormancy time, speeding up production cycle, and achieving remarkable effects.

Active Publication Date: 2022-03-22
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the lily seedlings propagated by tissue culture need to grow into small bulbs through multiple steps before they can be transplanted into soil or planting substrates, and lilies belong to perennial herbaceous plants, and their bulbs have dormant characteristics, so the tissue After the cultured seed bulbs are out of the bottle, they must break dormancy to germinate and grow in the soil, and the breaking of the dormancy of the seed bulbs still needs to be refrigerated at a low temperature of 0-4°C for 3-4 months, so this patent does not solve how to germinate and grow in the soil. The problem of making lily tissue culture seed bulbs break dormancy and be quickly transplanted to the field for growth and breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for quickly breaking the dormancy of the bulbs of lily lily seeds, characterized in that it comprises the following steps:

[0022] A. Take out the lily lily seed bulb with a circumference of 1.0 cm obtained by tissue culture and breeding, wash it 3 times with sterile water, remove the medium residue attached to the seed bulb, and keep the root tissue of the seed bulb;

[0023] B. The seed bulbs cleaned in step A were treated in a water bath with warm water at 32°C for 60 minutes, and the water temperature was kept constant at 32°C during the water bath treatment, and dried naturally at room temperature for 24 hours to remove the moisture on the surface of the seed bulbs;

[0024] C. Soak the seed bulbs of step B in a thiadiuron solution with a concentration of 1.0 mg / L, place it at room temperature for 48 hours, take it out, and perform the following sterilization treatment: use 50% carbendazim wettable powder as the solute, solvent For sterile water, prepare ...

Embodiment 2

[0027] A method for quickly breaking the dormancy of the bulbs of lily lily seeds, characterized in that it comprises the following steps:

[0028] A, take out the lily lily seed bulb with a girth of 2.0 cm obtained by tissue culture and breeding, wash it 3 times with sterile water, remove the medium residue attached to the seed bulb, and retain the root tissue of the seed bulb;

[0029] B. The seed bulbs cleaned in step A are treated in a water bath with warm water at 35° C. for 30 minutes. During the water bath treatment, the water temperature is kept constant at 35° C., and dried naturally for 24 hours at room temperature to remove the moisture on the surface of the seed bulbs;

[0030] C. Soak the seed bulb of step B in a thiadiuron solution with a concentration of 6.0 mg / L, place it at room temperature for 12 h, take it out, and perform the following sterilization treatment: use 50% carbendazim wettable powder as the solute, The solvent is sterile water, a suspension with...

Embodiment 3

[0033] A method for quickly breaking the dormancy of the bulbs of lily lily seeds, characterized in that it comprises the following steps:

[0034] A, take out the lily lily seed bulb with a diameter of 1.5cm that is obtained by tissue culture and breeding, wash it 3 times with sterile water, remove the medium residue attached to the seed bulb, and keep the root tissue of the seed bulb;

[0035] B. The seed bulbs cleaned in step A were treated in a water bath with warm water at 33°C for 48 minutes, and the water temperature was kept constant at 33°C during the water bath treatment, and dried naturally at room temperature for 24 hours to remove the moisture on the surface of the seed bulbs;

[0036] C. Soak the seed bulbs of step B in a thiadiuron solution with a concentration of 4.0 mg / L, place it at room temperature for 24 hours, take it out, and perform the following sterilization treatment: use 50% carbendazim wettable powder as the solute, solvent For sterile water, prepar...

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Abstract

The invention provides a method for rapidly breaking the dormancy of lily bulbs of green flowers, comprising the following steps: taking out the bulbs of lily seeds of green flowers obtained by tissue culture and breeding, washing them with warm water at 32-35°C for 30-60 minutes, and then Dry naturally at room temperature for 24 hours, soak in thiadizuron solution with a concentration of 1.0-6.0 mg / L, place it at room temperature for 12-48 hours, take it out, sterilize it, and place it under full light for 7-15 days. Dormant seed bulbs can be transferred to soil or planting substrate for cultivation. The present invention is not limited by the season when the tissue cultured bulbs come out of the bottle, saves 60-90 days compared with the conventional dormancy, greatly shortens the dormancy time of the green lily, realizes the large-scale and rapid breeding of the green lily, and makes the green lily seed bulb grow within 30 days. Neat germination, the germination rate reaches more than 80%, and solves the problem of long breeding cycle of green lily in the prior art.

Description

technical field [0001] The invention relates to a method for quickly breaking dormancy of lily seed bulbs, and belongs to the technical field of plant cultivation. [0002] technical background [0003] Green Lily ( Lilium fargesii Franch.) is a plant of the genus Lilium in the family Liliaceae endemic to China. Distributed in warm and humid hillside forests at an altitude of 2000-2800 meters in Yunnan, Sichuan, Hubei and Shaanxi. The flowers of the green lily are green and white, with purple-brown spots on the petals. Not only are the flowers beautiful and fragrant, they are highly ornamental, but the bulbs of the green lily are also a good product for both medicine and food. Due to the long reproductive cycle of wild green lily plants, the bulbs are difficult to germinate and grow slowly due to dormancy, and people’s long-term over-harvesting has led to a sharp decline in the population, making it difficult to find green lily at present. wild plants. For this reason, af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 崔光芬杜文文郭燕吴学尉王祥宁段青贾文杰马璐琳王继华
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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