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A kind of salvia miltiorrhiza extract, injection and application thereof

A technology for extracts and injections, which is applied in the field of medicinal chemistry to achieve the effects of enhancing pharmacological activity, high medicinal efficacy and good therapeutic effect.

Active Publication Date: 2021-10-01
SHANGHAI TREEFUL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of salvianolic acid extracts in sleep apnea hypopnea syndrome complicated with hypertension has not been reported yet

Method used

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  • A kind of salvia miltiorrhiza extract, injection and application thereof
  • A kind of salvia miltiorrhiza extract, injection and application thereof
  • A kind of salvia miltiorrhiza extract, injection and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of salvia miltiorrhiza extract, comprising:

[0036] To activate the strain, inoculate Beauveria bassiana on the SDA medium by streaking method, and place it in an incubator at 28°C for 7 days to obtain the activated strain;

[0037] Strain expansion, the above-mentioned activated strains were inoculated in Sabouraud dextrose liquid medium, the temperature was set at 28°C, the rotation speed of the shaker was 180rpm, and cultured for 48 hours to obtain the strain expansion culture medium;

[0038] The medicinal material is fermented, the salvia miltiorrhiza is crushed into powder, and tap water (solid-to-liquid ratio 1:2g / mL) is added to moisten the medicinal material, and left overnight. Sterilize at 121°C for 30 minutes, add strain expansion culture solution containing 0.05% cryptochlorogenic acid (solid-to-liquid ratio: 15:1g / mL) after the medicinal materials are cooled, and ferment in an incubator at 28°C for 30 days. Gently shake the medicinal...

Embodiment 2

[0052] A preparation method of Danshen extract differs from Example 1 in that cryptochlorogenic acid is not added during the fermentation process of medicinal materials; the extraction rate of Danshen extract is 76.8%.

[0053] 1. Extraction rate of Salvia miltiorrhiza extract

[0054] Comparing Example 1 and Example 2, it can be seen that the extraction rate of the Danshen extract in Example 1 is higher than that in Example 2, indicating that the addition of cryptochlorogenic acid can improve the extraction rate of the Salvia miltiorrhiza extract.

[0055] 2. Determination of some components of Danshen extract

[0056] Same as Example 1. The measured results were: salvianolic acid B glycosyl compound, 52.9%; salvianolic acid Y glycosyl compound, 4.53%; rosmarinic acid glycosyl compound, 3.21%.

[0057] Comparing Example 1 and Example 2, it can be seen that in the Salvia miltiorrhiza extract prepared in Example 1, the content of the above-mentioned 3 components is significan...

Embodiment 3

[0059] Extracellular Enzyme Activity Assay

[0060] 1. Liquid culture of bacterial strains and preparation of crude enzyme solution

[0061] Culture Beauveria bassiana strain on SDA plate at (25±1)°C constant temperature for 10 days, scrape spore powder under aseptic conditions, and place it in a tissue grinder filled with a small amount of sterile water containing 0.05% Tween-80 After being fully ground in the medium, pour it into a sterilized small beaker, count with a hemocytometer, and adjust the concentration of the spore suspension to 100 million / mL for later use;

[0062] Take two equal parts of the above-mentioned spore suspension and inoculate it into 100mL SDB medium (placed in a 250mL Erlenmeyer flask) with 1% amount, one part is added with 0.05% cryptochlorogenic acid (experimental group), and one part without (control group); cultured in a constant temperature shaker at (25±1)°C at 150r / min for 5 days, and left the filtrate after filtration to measure the activit...

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Abstract

The invention discloses a salvia miltiorrhiza extract, an injection and an application thereof, and relates to the technical field of medicinal chemistry. The preparation method of the salvia miltiorrhiza extract specifically comprises: activating the strains, inoculating Beauveria bassiana on SDA medium for cultivation; amplifying the strains, inoculating the above-mentioned activated strains in a liquid medium and culturing them to obtain the strains culture medium; the medicinal material is fermented, the salvia miltiorrhiza is crushed into powder, water is added, sterilized, and the bacterial culture liquid containing cryptochlorogenic acid is added for fermentation to obtain the fermented medicinal material; the fermented medicinal material is obtained by separating and purifying, soaking the above-mentioned fermented medicinal material with methanol aqueous solution, ultrasonication, filtration, reduction The extract of Danshen was obtained by rotary steaming. The prepared salvia miltiorrhiza extract has high yield, excellent pharmacological activity and enhanced anti-inflammation effect; it can be made into injections for treating obstructive sleep apnea hypoventilation syndrome combined with hypertension, and the effect is remarkable.

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry, and specifically relates to a salvia miltiorrhiza extract, an injection and uses thereof. Background technique [0002] Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge., a plant of the labiatae family Labiatae. Because the root bark is red and the flesh is purple, and it has nourishing and strong effects, it is named Danshen. It was first recorded in the "Shen Nong's Herbal Classic" in the Han Dynasty and was Listed as top grade, it is an essential medicine for promoting blood circulation and regulating menstruation. Its main medicinal substances are water-soluble salvianolic acid and fat-soluble tanshinone compounds. Salvia miltiorrhiza belongs to traditional Chinese medicine, which has the functions of promoting blood circulation and removing blood stasis, stimulating menstruation and relieving pain, cooling blood and eliminating carbuncle, and has signif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K125/00A61K31/7034
CPCA61K9/0019A61K31/7034A61K36/537A61K47/02A61K2236/19A61K2236/333A61P9/12A61P11/00A61P25/20A61P29/00A61K2300/00
Inventor 黄生
Owner SHANGHAI TREEFUL PHARMA
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