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Preparation method and application of efficient microbial agent for slope protection

A microbial agent and high-efficiency microbial technology, applied in the field of preparation of high-efficiency microbial agents, can solve problems such as increased difficulty in slope protection, weakened water storage capacity, soil pollution, etc., to improve soil enzyme activity, increase survival rate, and increase survival rate rate effect

Active Publication Date: 2020-12-18
北京航天恒丰科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, after the slope soil degrades, it becomes sandy and the water storage capacity is weakened; while the roots of slope protection plants grow slowly at the initial stage of planting, the degraded soil is not conducive to plant seed implantation and plant rooting and germination, which leads to a decrease in survival rate. Increased the difficulty of slope protection and weakened the ecological effect of slope protection
[0004] If a large amount of compound fertilizer is used to improve the survival rate of slope protection plants, there will be serious leaching of fertilizer nutrients, which will further damage the water environment around the pasture; although organic fertilizers mainly based on farmyard manure can improve soil physical and chemical properties, the organic matter properties are mixed. The germs and insect eggs contained in it are easy to cause soil pollution, and it is not suitable for pasture soil after soil degradation

Method used

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  • Preparation method and application of efficient microbial agent for slope protection
  • Preparation method and application of efficient microbial agent for slope protection
  • Preparation method and application of efficient microbial agent for slope protection

Examples

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preparation example 1

[0034] Inoculate the Bacillus amyloliquefaciens FZB42 strain on the LB solid plate medium for streak culture to activate the strain; wherein, the LB solid plate medium is tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L L was obtained by adding agar after distilled water was used to make up the volume.

[0035] The activated colony was inoculated into LB liquid medium, and cultured at 30°C for 15 hours to obtain the first-grade seed liquid preparation of Bacillus amyloliquefaciens;

[0036] The primary seed liquid of Bacillus amyloliquefaciens was subcultured in 100ml of LB liquid medium according to the ratio of 1:100 to prepare the secondary seed liquid of Bacillus amyloliquefaciens;

[0037] Inoculate the secondary seed liquid of Bacillus amyloliquefaciens into the fermentation medium, shake and culture at 32°C for 48h, wash with sterile water, collect the bacteria after centrifuging at 4°C and 5000r / min for 10min, and resuspend the bacteria in In sterile double d...

preparation example 2

[0044] Inoculate the Bacillus amyloliquefaciens FZB42 strain on the LB solid plate medium for streak culture to activate the strain; inoculate the activated colonies into the LB liquid medium to prepare the primary seed liquid of Bacillus amyloliquefaciens; The primary seed liquid of Bacillus amyloliquefaciens was subcultured in LB liquid medium to prepare the secondary seed liquid of Bacillus amyloliquefaciens; the secondary seed liquid of Bacillus amyloliquefaciens was inoculated into the fermentation medium, shaken at 32°C for 48 hours, and washed with sterile water Finally, centrifuge at 4°C and 5000r / min for 10 minutes to collect the bacteria, resuspend the bacteria in sterile double-distilled water, and adjust the concentration of the bacteria suspension to 6.5×10 9 cfu / ml, spray-dried to obtain Bacillus amyloliquefaciens inoculum; wherein, the components of the fermentation medium include: 25 parts of corn flour, 18 parts of cottonseed meal, 18 parts of bran, KH 2 PO 4...

preparation example 3

[0048] After the Bacillus amyloliquefaciens is activated, it is inoculated into the liquid seed culture medium of the Bacillus amyloliquefaciens to prepare the primary seed liquid of the Bacillus amyloliquefaciens;

[0049] Cultivate to obtain Bacillus amyloliquefaciens fermentation seed liquid; wherein, the amount of Bacillus amyloliquefaciens bacteria is 1×10 8 cfu / ml;

[0050] The primary seed liquid of Bacillus amyloliquefaciens was subcultured in LB liquid medium to prepare the secondary seed liquid of Bacillus amyloliquefaciens;

[0051] The secondary seed liquid of Bacillus amyloliquefaciens was inoculated into the fermentation medium, shaken and cultivated at 32°C for 48 hours, washed with sterile water, centrifuged at 4°C and rotated at 5000r / min, and the bacteria were collected. Resuspend the bacteria in sterile double distilled water, adjust the concentration of the bacteria suspension to 6.5×10 9 cfu / ml, spray-dried to obtain Bacillus amyloliquefaciens inoculum; ...

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Abstract

The invention provides a preparation method and application of an efficient microbial agent for slope protection, and relates to the technical field of ecological restoration. The preparation method comprises the following steps of mixing a bacillus amyloliquefaciens agent, a trichoderma harzianum agent and a glomus mosseae agent according to a mass ratio of 2: 5: 49 to form mixed bacterial powder. The effects of degrading a straw plate and improving the survival rate of slope protection plants are optimal.

Description

technical field [0001] The invention relates to the technical field of ecological restoration, in particular to a preparation method and application of a high-efficiency microbial agent for slope protection. Background technique [0002] China's ecological environment is increasingly fragile. According to statistics, due to wind and sand activities, secondary salinization, and pasture overload and overgrazing, the soil quality of pastures is loose and landslides occur frequently, which not only increases the sediment concentration of rivers, And because livestock manure directly enters the river, it not only affects the environmental development of the pasture, but also threatens the ecological balance of the water environment around the pasture. Vegetation slope protection is the preferred ecological slope protection technology. [0003] However, after the slope soil degrades, it becomes sandy and the water storage capacity is weakened; while the roots of slope protection ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01B79/02A01G24/20A01G24/10A01G22/00A01G22/20A01G22/40C12R1/07C12R1/885C12R1/645
CPCA01B79/02A01G22/00A01G22/20A01G22/40A01G24/10A01G24/20C12N1/14C12N1/20
Inventor 刘海明李硕姜秀娟吕中文程淑琴杜蓉蓉
Owner 北京航天恒丰科技股份有限公司
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