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A kind of method for preparing agarose

A technology of agarose and colloid, which is applied in the field of preparation of agarose, can solve the problems of cumbersome production process, high cost, long production cycle, etc., achieve good separation effect, improve sample quality, and reduce the effect of intermediate production links

Active Publication Date: 2022-07-19
SHANGHAI OPM BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has certain disadvantages in the process of producing agarose. The raw material agar often needs to be extracted from seaweed by traditional alkali treatment process to make agar powder and then further modified. There are problems such as long production cycle, cumbersome production process and high cost.

Method used

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  • A kind of method for preparing agarose
  • A kind of method for preparing agarose
  • A kind of method for preparing agarose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Different pH

[0059] Alkali treatment: Rinse the gracilis with tap water, dry it naturally, take 100g of the dried grass, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) algae water Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, use tap water for soaking and rinsing, with an interval of 40 minutes each time, until the pH of the lotion is neutral.

[0060] Acidification treatment: add sulfuric acid, oxalic acid and disodium EDTA to the rinsed grass according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system, Acidify for 40min, pour off the liquid, soak and rinse until the pH of the lotion is neutral.

[0061] Bleaching treatment: add water to the acidified grass, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 min, and then soak and rinse until the pH of the lotion is neutral.

[0062] Boiled gelatin: After washing, the gelatin was boiled for 1.5h at 100°C with a r...

Embodiment 2

[0067] Embodiment 2 Different hydrogen peroxide addition amounts

[0068] Alkali treatment: Rinse the gracilis with tap water, dry it naturally, take 100g of the dried grass, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) algae water Constant temperature treatment at 90°C for 3h. After the alkali treatment is completed, use tap water for soaking and rinsing, with an interval of 40 minutes each time, until the pH of the lotion is neutral.

[0069] Acidification treatment: add sulfuric acid, oxalic acid and disodium EDTA to the rinsed grass according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system, Acidify for 40min, pour off the liquid, soak and rinse until the pH of the lotion is neutral.

[0070] Bleaching treatment: add water to the acidified grass, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 min, and then soak and rinse until the pH of the lotion is neutral.

[0071] Boiled gelatin: After washing, the gelatin wa...

Embodiment 3

[0076] Example 3 Different reaction times

[0077] Alkali treatment: Rinse the gracilis with tap water, dry it naturally, take 100g of the dried grass, add 7% (w / v) NaOH solution at a ratio of 1g:20mL (m:v) algae water Constant temperature treatment at 90°C for 3h. After the alkali treatment, use tap water for soaking and rinsing, with an interval of 40 minutes each time, until the pH of the lotion is neutral.

[0078] Acidification treatment: add sulfuric acid, oxalic acid and disodium EDTA to the rinsed grass according to the ratio of 0.064% (v / v), 0.052% (m / v) and 0.012% (m / v) of the total system, Acidify for 40min, pour off the liquid, soak and rinse until the pH of the lotion is neutral.

[0079] Bleaching treatment: add water to the acidified grass, then add 0.1% (w / v) sodium hypochlorite solution, bleach for 40 min, and then soak and rinse until the pH of the lotion is neutral.

[0080] Boil the gelatin: After washing the grass, boil the gelatin at 100℃ for 1.5h with...

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Abstract

The present invention relates to a method for preparing agarose. The method comprises the following steps: washing grass, performing alkali treatment, acidizing treatment, bleaching treatment, boiling gelatin, filtering, cooling and solidifying, so as to obtain colloid; ;Add water to the crushed colloid, heat it, adjust the pH, and then add H 2 O 2 The solution was reacted, filtered and washed after the reaction, and washed repeatedly until the colloidal sample was free of H 2 O 2 Residual and the pH of the lotion is neutral, the sample is then frozen, dehydrated, dried and pulverized to obtain agarose; this method can significantly reduce the content of sulfuric acid groups in the agar, increase the gel strength, and significantly reduce the electroendosmosis. The commercially available agarose requires short reaction time and environmental protection.

Description

technical field [0001] The invention relates to the technical field of deep processing of seaweed, in particular to a method for preparing agarose. Background technique [0002] Agarose is a polysaccharide polymer, the main component of agar, its basic structure is repeating alternating 1,3-linked β-D-galactose and 1,4-linked 3,6-anhydro-α-L - Galactose. Agarose is white or slightly yellow powder, odorless, tasteless, soluble in hot water, and soluble in dimethyl sulfoxide (DMSO). Compared with agar, agarose contains less negatively charged groups and has better properties. It has the characteristics of high gel strength, low sulfate content, and low electro-endosmosis. These properties also make agarose in the fields of biology and medicine. Wide range of applications. [0003] In the 1960s, a scientist in Sweden isolated agarose for the first time. Since then, researchers from various countries have begun to explore the preparation technology of agarose. At present, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/12
CPCC08B37/0003C08B37/0039
Inventor 肖安风张聪肖琼陈福泉张永辉杨秋明
Owner SHANGHAI OPM BIOSCI CO LTD
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