Preparation method of buckwheat husk non-flavonoid substance

A non-flavonoid and substance-free technology of buckwheat husks, which can be applied to medical preparations containing active ingredients, pharmaceutical formulas, drug combinations, etc., and can solve problems such as excessive production of ROS

Active Publication Date: 2022-02-15
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The continuous increase of blood sugar will in turn cause the excessive production of ROS

Method used

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  • Preparation method of buckwheat husk non-flavonoid substance
  • Preparation method of buckwheat husk non-flavonoid substance
  • Preparation method of buckwheat husk non-flavonoid substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of buckwheat hull non-flavonoid components

[0040] 1. Sample processing

[0041] Select natural buckwheat husks, remove impurities, dry, crush, and pass through a 0.25 mm (60 mesh) sieve; accurately weigh 1 g of buckwheat husk powder, place it in a 50 mL centrifuge tube, heat water to keep warm to extract water-soluble components, filter with suction, and the filtrate is fixed. The volume was reduced to 100mL to obtain a BHE solution, which was stored for future use.

[0042] 2. Extraction of non-flavonoid components from buckwheat hulls

[0043] 1. Pretreatment of macroporous resin

[0044] Soak D101 macroporous resin and AB-8 macroporous resin in 95% ethanol for 24 h to remove impurities. Ethanol was filtered off and washed with distilled water until odorless. Soak in 4% HCl and 4% NaOH solution for 24 hours in turn, wash with distilled water to pH 7.0 after each soaking, and finally soak in 95% ethanol, save for future use.

[0045] 2. Prima...

Embodiment 2

[0049] Example 2 Separation and purification of non-flavonoid substances from buckwheat hulls

[0050] 1. Separation and purification

[0051] According to the principle of similar miscibility, using the different solubility of solutes in different organic solvents, the organic impurities mixed in the non-flavonoid components of buckwheat hulls are separated; the order of polarity of the main organic reagents is: water (maximum) > acetonitrile >methanol>ethanol>propanol>acetone>tetrahydrofuran>n-butanol>ethyl acetate>diethyl ether>isopropyl ether>dichloromethane>chloroform (chloroform)>bromoethane>benzene>carbon tetrachloride>n-hexane > Kerosene (minimum); In this experiment, chloroform, ethyl acetate, n-butanol and water, which are effective for most organic substances, were selected for three-phase extraction, and according to the polarity, chloroform, ethyl acetate, and n-butanol were used in sequence to achieve The effect of synergistic extraction.

[0052] Since there a...

Embodiment 3

[0060] Example 3 Determination of non-flavonoid substances in buckwheat husk

[0061] 1. Color reaction

[0062] Using ferric chloride reaction, the BHNF-1 component and BHNF-2 component were prepared into 1 mg / mL sample solution, respectively, and the ferric chloride solution was added to react, and the color change of the solution was observed.

[0063] Using hydrochloric acid-magnesium powder reaction, the BHNF-1 component and BHNF-2 component were prepared into 1 mg / mL sample solution, and a few drops of concentrated hydrochloric acid and a small amount of magnesium powder were added to react, and the color change of the solution was observed.

[0064] Results: In the ferric chloride color reaction, the solutions of BHNF-1 and BHNF-2 components became darker and brownish black, indicating that the non-flavonoid components of buckwheat hulls may contain phenolic or tannin components. However, in the hydrochloric acid-magnesium powder test, the BHNF-1 and BHNF-2 components ...

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Abstract

The invention provides a method for preparing buckwheat husk non-flavonoid substances, which comprises: extracting buckwheat husk with hot water to obtain a BHE solution; absorbing with D101 macroporous resin, eluting with ethanol, collecting the eluate, and freeze-drying to obtain D101 Refined macroporous resin; dissolved in 15-20mL water to obtain PBHE solution, adsorbed with AB‑8 macroporous resin; washed with water; eluted with eluent, collected eluate, concentrated, dried; redissolved in Water, standing still, filtering, removing insoluble impurities, adding the solution to the extraction device; adding organic solvent, shaking fully immediately, standing still, separating liquid, and retaining the water layer components; concentrating, freeze-drying, and obtaining non-flavonoid substances of buckwheat husk ; Advantages of the present invention: buckwheat husk non-flavonoid material has stronger free radical scavenging activity and total antioxidant capacity, to H 2 o 2 The induced oxidative damage of HepG2 cells has obvious cytoprotective effect; it has anti-diabetic activity.

Description

technical field [0001] The invention belongs to the field of health food and medicine, and in particular relates to a preparation method of buckwheat hull non-flavonoid substances. Background technique [0002] Buckwheat ( Fagopyrum esculentum ), a herbaceous dicotyledonous cereal crop, as a medicinal and edible homologous plant, has high nutritional and economic value. my country's buckwheat production ranks second in the world after Russia. Buckwheat is rich in a variety of nutritional components such as starch, protein, fat, vitamins and minerals, and the content is higher than that of bulk and small grains. It also contains many therapeutic components such as flavonoids, polyphenols, active peptides , fatty acids, phytosterols and D-chiro-inositol, etc. Epidemiology shows that regular consumption of buckwheat can effectively reduce the incidence of hyperglycemia, and it can be developed as a green functional food. Buckwheat husk, as the most abundant by-product in bu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/70A61P3/10A61P39/06
CPCA61K36/70A61P3/10A61P39/06A61K2236/331A61K2236/55A61K2236/53A61K2236/51
Inventor 朴春红崔阳郭阳王玥刘子琦王秀娟王玉华刘俊梅于寒松代伟长
Owner JILIN AGRICULTURAL UNIV
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