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Application of IER3 as biomarker in early prediction of sepsis

A biomarker and sepsis technology, applied in the biological field, can solve the problems of large changes in results, short retention time of inflammatory cytokines, difficult detection timing, etc., and achieve the effect of improving prognosis and reducing the incidence of sepsis.

Pending Publication Date: 2020-12-01
中国人民解放军陆军特色医学中心
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Clinically used biomarkers mainly include C-reactive protein (CRP), procalcitonin (PCT), etc., but these indicators can only reflect the degree of systemic inflammatory response in patients at the time of detection, and with different detection time windows, The results vary greatly, and their specificity is not high; inflammatory cytokines such as IL-6 and TNFα have a short retention time in the blood, and it is difficult to accurately grasp the appropriate timing of detection; and scoring systems based on body anatomy and pathophysiology, such as AIS , ISS, SOFA, APACH III, etc., cannot reflect the disease development and prognosis of trauma patients
Therefore, these indicators can only be used as auxiliary indicators for the diagnosis and monitoring of trauma sepsis. So far, no specific biomarkers for the early prediction of trauma sepsis have been found. Therefore, new biomarkers with high sensitivity and specificity have been found. The application of biomarkers in the early prediction of trauma sepsis is very important for its early detection, early diagnosis and early treatment

Method used

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  • Application of IER3 as biomarker in early prediction of sepsis
  • Application of IER3 as biomarker in early prediction of sepsis
  • Application of IER3 as biomarker in early prediction of sepsis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Detection of plasma IER3 level in trauma patients and its correlation analysis with the occurrence of sepsis

[0033] Peripheral blood samples were collected from 243 patients with severe multiple injuries on the day of injury (Table 1), and were divided into a sepsis group of 67 cases and a non-sepsis group of 176 cases according to whether sepsis occurred. company, product number abx252651) to detect plasma IER3 expression, the kit uses double antibody sandwich ELISA method. The anti-human IER3 antibody is used to coat the microtiter plate, and the human IER3 in the sample or standard is combined with the coated antibody during the experiment, and the free components are washed away. Biotinylated anti-human IER3 antibody and horseradish peroxidase-labeled avidin were added sequentially. The anti-human IER3 antibody binds to the human IER3 bound to the coated antibody, biotin and avidin specifically bind to form an immune complex, and the free components are washed aw...

Embodiment 2

[0040] Further use of ROC curve to analyze the early predictive value of plasma IER3 on the risk of sepsis

[0041] According to the IER3 expression level value detected in the blood plasma of trauma patients in Example 1, the ROC curve was drawn using SPSS22.0 software.

[0042] figure 2 Shown is a receiver operating curve (ROC) analysis of the early prediction effect of plasma IER3 on the risk of sepsis. The results showed that the area under the curve (AUC) of IER3 was 0.81, indicating that IEX has a good predictive value for early sepsis. AUC reflects predictive power (AUC=0.5, no predictive power: 0.5<AUC<0.7, little predictive value: 0.7<AUC<0.9 fairly accurate predictive value: 0.9<AUC<1, very accurate predictive value).

Embodiment 3

[0044] Detection of IER3 expression in various tissues and organs of mice:

[0045] Select adult C57 mice, and first establish a sepsis model. The sepsis model is established by cecal ligation and puncture (CLP). Anesthetized by intraperitoneal injection of barbital sodium at a concentration of 40 mg / kg, fixed on the operating board, shaved and disinfected the skin on the abdomen, made a 1 cm long incision in the middle of the abdomen, found the colon ligation, punctured through the cecum at the ligated end with a 26-gauge syringe needle, and put it back The wound was sutured retroperitoneally and the skin was disinfected. This method is a classic modeling method for sepsis animal models, and this model is also a standard animal method for sepsis research. Divided into 2 groups according to sepsis and sham operation (only laparotomy and laparotomy were performed), and mouse plasma, liver, lung, kidney and spleen tissues were obtained 12, 24, and 72 hours after model establish...

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Abstract

The invention relates to the technical field of biology, in particular to application of an early response gene 3 (IER3) as a biomarker in a reagent or a kit for early prediction of sepsis. The IER3 is adopted as a biomarker, and crowd experiments prove that the IER3 level of plasma of a patient suffering from severe trauma sepsis is remarkably higher than that of a patient without sepsis. By detecting the plasma IER3 level, early prediction is conducted on the sepsis occurrence risk of a severely wounded patient so as to give individualized interventional therapy, the occurrence rate of post-traumatic sepsis can be reduced, and the important clinical application value can be achieved for improving prognosis of the severely wounded patient.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the application of an early response gene 3 as a biomarker in the early prediction of sepsis. Background technique [0002] Trauma is a global public health problem, and the number of deaths due to trauma is as high as 5 million every year, accounting for 10% of the total global disease burden. With the advancement of pre-hospital first aid technology and the improvement of the monitoring and treatment system, the direct mortality rate caused by injuries in the early stage of trauma has been significantly reduced, but complications such as sepsis and multiple organ dysfunction in the post-trauma period are still a serious threat patient survival. [0003] Sepsis is a life-threatening organ dysfunction caused by the dysregulation of the host's immune response to infection. It is a common serious complication and the main cause of death in critically ill patients with infection, burn / tra...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G01N33/535G01N33/543
CPCC12Q1/6883G01N33/54306G01N33/535C12Q2600/158G01N2800/26Y02A50/30
Inventor 张安强蒋建新陆红祥文大林杜娟张华才孙剑会曾灵刘迪
Owner 中国人民解放军陆军特色医学中心
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