Traditional Chinese medicine active ingredient targeted screening method and kit

Abstract

The invention discloses a traditional Chinese medicine active ingredient targeted screening method and a kit. According to the targeted screening method, a traditional Chinese medicine active ingredient targeted screening medium is based on ultrahigh affinity acting force between an inactive mutant CL7 of colistin E7 DNA enzyme and homologous immune protein Im7 of the inactive mutant CL7; therefore, one-step reversible immobilization of fusion of the CL7 label in the cell lysis solution and the GPCRs is realized, and thus the targeted screening method integrates ultrahigh affinity between Im7and CL7 and high specificity of receptor recognition drugs, and high-specificity targeted rapid screening of GPCRs targeted traditional Chinese medicine active ingredients can be realized.

Description

technical field [0001] The present invention relates to a targeted screening method and kit for active components of traditional Chinese medicine, in particular to a targeted screening method and kit for active components of traditional Chinese medicine with G protein coupled receptors (GPCRs) in organisms as the target. The technical field of targeted screening of pharmaceutical active ingredients. Background technique [0002] At present, the commonly used methods for preparing solid-phase carriers for screening active pharmaceutical ingredients are usually to immobilize cells, cell membranes, and proteins on the surface of solid substrates. The immobilization methods used mainly include: random immobilization methods, directional immobilization methods, etc. After long-term research, it is found that these methods have the following common deficiencies: [0003] 1. There are multiple drug targets on the surface of cells or cell membranes, and the active ingredients scree...

AI Technical Summary

Problems solved by technology

[0003] 1. There are multiple drug targets on the surface of cells or cell membranes, and the active ingredients screened are complexes, so the specific targets of the screened active ingredients cannot be clarified;

[0004] 2. Immobilized proteins usually focus on soluble proteins (such as enzymes), and there are fewer studies on G protein-coupled receptors, the largest in vivo target family of drugs;

[0005] 3. The reusability of the immobilized solid matrix cannot be realized, making it difficult to achieve large-scale preparation and application of the immobilized solid matrix, and the consumption of raw materials is large

[0020] The directional immobilization method has certain advantages in ensuring the biological activity and stability of the immobilized protein. However, because the immobilized matrix cannot be reused, this method has the problem of high raw material consumption.

Images