A kind of zwitterionic polymer for implantable sensor and preparation method thereof

A technology of zwitterions and polymers, which is applied in the direction of instruments, scientific instruments, analytical materials, etc., can solve the problems of increasing the production cycle and cost of sensors, reducing the concentration of analytes, etc., to improve detection sensitivity and accuracy, and strong anti-interference ability , The effect of simple production process

Active Publication Date: 2022-05-17
杭州柏医健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the external coating film improves the biocompatibility of the sensor, it also reduces the concentration of the analyte; the additional coating process also increases the production cycle and cost of the sensor

Method used

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  • A kind of zwitterionic polymer for implantable sensor and preparation method thereof
  • A kind of zwitterionic polymer for implantable sensor and preparation method thereof
  • A kind of zwitterionic polymer for implantable sensor and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 SBMA and NH 2 -(CH 2 ) 6 (m-bim)Os(bpy) 2 2PF 6 Preparation of Zwitterionic Polymers by Polymerization

[0055] The thiobetaine methyl acrylate (SBMA) used in this example was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., the osmium complex NH 2 -(CH 2 ) 6 (m-bim)Os(bpy) 2 2PF 6 For the synthesis of this research group, refer to the synthetic route in AnIntrinsic Self-Charging Biosupercapacitor Combined of a High-Potential Bioanode and a Low-Potential Biocathode, other reagents are known products, obtained by purchasing commercially available products.

[0056] (1) Copolymerization of acrylic acid (AA) and thiobetaine methyl acrylate (SBMA) monomer solution: Weigh 2.21g of AA and 0.04g of SBMA monomer in turn in a polymerization bottle filled with a certain amount of 0.3M NaCl solution, shake to make it Completely dissolved, nitrogen at room temperature. After adding 0.0018g of potassium persulfate and sodium bisulfite (mass ratio...

Embodiment 2

[0058] Example 2 Electrochemical characterization (CV) of p(SBMA-AA-Os) modified electrode

[0059] Using the p(SBMA-AA-Os) prepared in Example 1, prepare a pbs solution of 10 mg / ml p(SBMA-AA-Os), pipette 2 microliters of the solution to the surface of the glassy carbon electrode and dry it as a membrane-modified electrode , the electrochemical experiment was carried out on Chenhua CHI600 electrochemical workstation, 10mL electrolytic cell, three-electrode system: the membrane-modified glassy carbon electrode was used as the working electrode, Ag / AgCl was used as the reference electrode, the platinum wire electrode was used as the counter electrode, and the bottom solution was tested It is a phosphate buffer solution of pH 7.4, and the result of cyclic voltammetry is as follows figure 2 Shown, and compared with commonly used ferrocene-modified electrodes.

[0060] figure 2 The voltammogram of the zwitterionic polymer clearly shows specific peaks for the oxidation and subse...

Embodiment 3

[0061] Example 3 Stability test of p(SBMA-AA-Os) modified electrode

[0062] Using the p(SBMA-AA-Os) modified electrode prepared in Example 2, in the in vitro experiment, the stability test of the electrode was performed using a newborn bovine whole serum solution stored at 37° C. to simulate the in vivo environment. Impedance (Z')-time (T) scanning was performed on the p(SBMA-AA-Os) modified electrode in neonatal bovine serum at 37°C, and the results were as follows image 3 shown, reflecting its performance in an in vitro environment. Depend on image 3It can be seen that the impedance of the p(SBMA-AA-Os) modified electrode only increased slightly in the first few minutes, and then quickly reached equilibrium and remained stable at 230Ω; after the glassy carbon bare electrode GCE was immersed in serum, its impedance increased rapidly, from 330Ω It quickly rose to 550Ω, and the overall impedance was nearly doubled, and it began to stabilize after about 1 hour. It can be s...

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Abstract

The present invention provides a zwitterionic polymer used in implantable sensors, especially an implantable glucose sensor, and provides a preparation method for the polymer to obtain a zwitterionic polymer containing a redox ligand, It can solve the biocompatibility problem in the body on the inner layer of the sensor (inert electrode surface), and can quickly transfer electrons between the enzyme electrodes, making the measurement results reliable and stable, and solving the problem of adding coating on the outer surface of the sensor for implantable glucose sensors. In order to solve the problem of layer technology, the enzyme electrode for implantable sensor can be prepared in one step. The process is simple, it can be used directly in vivo or in complex solution environment, and the detection sensitivity and accuracy of implantable sensor can be significantly improved.

Description

technical field [0001] The invention relates to the technical field of polymer preparation, in particular to a zwitterionic polymer used for implanted sensors and a preparation method thereof. Background technique [0002] With the improvement of living standards and the rapid development of microelectronics technology, implantable sensors for real-time monitoring of analyte concentration have attracted more and more attention. Such sensors can convert biochemical reaction signals based on analyte concentration into electrical or optical signals, and are widely used in the detection of analytes in clinical, environmental, agricultural, and biotechnological applications. Analytes that can be measured by implantable sensors in the clinical analysis of body fluids include glucose, lactate, cholesterol, bilirubin, and amino acids, among others. [0003] Implantable sensors that monitor analytes via electrical signals have attracted great attention because electron transfer is i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F220/06C08F220/38C08F8/32G01N27/327
CPCC08F220/06C08F8/32G01N27/3271C08F220/387
Inventor 王晓东陈玮宫宇山
Owner 杭州柏医健康科技有限公司
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