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A kind of silk protein/gelatin composite material and its application

A composite material and silk protein technology, which is applied in the field of silk protein/gelatin composite materials and the preparation of internal fixation systems, can solve the problems of thermal stability, swelling in vivo and unsatisfactory mechanical stability, etc., to promote cell growth and The effect of tissue recovery, high mechanical strength, and not easy to break nails

Active Publication Date: 2022-06-21
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the solutions of thermal stability, swelling in vivo and mechanical stability of silk protein / gelatin composites under physiological conditions by using enzymes, chemical reagents and physical cross-linking methods are still not ideal.
Based on the above problems, there are no reports on internal fixation systems such as bone nails prepared by silk protein / gelatin composite materials at home and abroad.

Method used

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  • A kind of silk protein/gelatin composite material and its application
  • A kind of silk protein/gelatin composite material and its application
  • A kind of silk protein/gelatin composite material and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of silk protein / gelatin composite material

[0052] see figure 1 , the preparation of silk protein / gelatin composite material includes the following steps:

[0053] 1. Degumming the natural silkworm cocoons with a sodium carbonate aqueous solution with a concentration of 0.5% and drying them to make degummed silk; then according to the mass ratio of silkworm cocoons: lithium bromide solution 1:10, dissolve with a concentration of 0.8% lithium bromide solution, dialysis desalination, Concentrated to make a 10% wt regenerated silk protein solution.

[0054] 2. Mix 9 mL of silk protein solution obtained in step 1 and 1 mL of 30% wt gelatin solution, then add 900 U / mL horseradish peroxidase and 0.5% v / v hydrogen peroxide solution (concentration 30%) and 20000 U Glutamine transaminase / mL, and gelatinized in a water bath at 37°C after mixing.

[0055] 3. The silk protein / gelatin gel obtained in step 2 is soaked in 70% alcohol for 24-36 hours, and then...

Embodiment 2

[0057] Example 2 Processing of Bone Nails and Bone Plates

[0058] The silk protein / gelatin composite material obtained in Example 1 was processed into columnar or massive mother embryos, and then processed into bone nails and bone plates by a multi-axis CNC machining lathe to obtain degradable silk protein / gelatin composite bone nails and bone nails. Internal fixation system consisting of bone plates. figure 2 A photograph of the bone screw is shown, which is a silk protein / gelatin composite screw. The size of the bone nail is: the diameter of the screw is 2 mm, the length of the screw is 5 mm, and the diameter of the end cap is 3.6 mm.

[0059] For comparison, regenerated silk protein (RSF) material and PLGA were used as raw materials to manufacture screws of the same size using a multi-axis CNC machining lathe.

Embodiment 3

[0060] Example 3 Mechanical test of external pull-out force of screws

[0061] For the silk protein / gelatin composite screw (RSF / G), regenerated silk protein screw (RSF) and PLGA screw obtained in Example 2, the pull-out force in the dry state and the wet state were tested according to the following methods.

[0062] According to American Society for Testing and Materials (ASTM) standard F1839, using an Instron 5966 (Instron, USA) universal chemistry instrument, a custom-made screw cap stainless steel holder and an artificial bone block (Sawbones, USA) paired The pullout force strength of RSF / G, RSFRSF / G and PLGA screws was tested. Artificial bone blocks were pre-embedded in bone cement, fixed to the bottom of an Instron 5966 test frame, and drilled with a drill (1.7 mm diameter). Screw the RSF / G and RSF screws into the artificial bone block, and then connect the Instron mechanics after clamping the screw cap with a holder. The screw was gradually pulled out of the artificia...

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Abstract

The invention discloses a silk protein / gelatin composite material, which is prepared by a method comprising the following steps: immersing silkworm cocoons in a soap salt solution for degumming, drying to make degummed silk, dissolving the degummed silk in a lithium bromide solution, and dialysis for desalting , concentrated to make a silk protein solution; mix the silk protein solution with the gelatin solution, add horseradish peroxidase, hydrogen peroxide solution and transglutaminase, and form a gel after mixing to obtain a silk protein / gelatin gel; The silk protein / gelatin gel is respectively soaked in alcohol and ammonium sulfate solution to obtain a silk protein / gelatin cross-linked gel, which is then dried. The silk protein / gelatin composite material can be used to prepare internal fixation systems used in trauma orthopedics and maxillofacial plastic surgery, and has high industrial production and clinical application prospects.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a silk protein / gelatin composite material and its use in preparing an internal fixation system. Background technique [0002] At present, the internal fixation system of metal materials is still the gold standard in the field of bone repair. This kind of material has good strength and plasticity, which is convenient for processing. At the same time, it is not easy to corrode in the human body and can exist stably in the body for a long time. However, the elastic modulus of traditional metal materials and human bone tissue is very different. After implantation, stress shielding effect occurs, which affects the process of bone healing or leads to osteoporosis. In addition, there are problems such as foreign body sensation and temperature allergy. Moreover, it needs to be removed by a second operation after bone healing, which increases the surgical risk and economic burden of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/12A61L31/14C08J3/075C08J3/24C08L89/00
CPCA61L31/129A61L31/148A61L31/14C08J3/075C08J3/246C08J2389/00C08J2489/00
Inventor 江立波董健李熙雷苏帝翰张其琛林红张泰维周雷
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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