A method for constructing a latent infection model of cytomegalovirus in the lungs of mice

A cytomegalovirus and construction method technology, which is applied in the field of construction of a mouse lung cytomegalovirus latent infection model, can solve the problems of few evaluation methods, short construction time, and molecular level evaluation of MCMV latent infection status, etc. Long duration, comprehensive assessment methods, and novel construction methods

Active Publication Date: 2022-07-08
张志辉
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Problems solved by technology

However, there is no relevant report on the establishment of an experimental mouse model of latent lung infection with MCMV, and due to differences in organ tissues and detection substances, the above existing methods are not suitable for the establishment of latent infection of lung MCMV , and the above existing methods do not evaluate the latent infection status of MCMV at the molecular level, and the construction time is too short and the evaluation methods are few, which cannot fully evaluate the influence of MCMV on the body tissue

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  • A method for constructing a latent infection model of cytomegalovirus in the lungs of mice
  • A method for constructing a latent infection model of cytomegalovirus in the lungs of mice

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Embodiment 1

[0036] Example 1 Establishment and evaluation of the latent infection model of pulmonary cytomegalovirus in mice

[0037] 1. Establishment of the latent infection model of cytomegalovirus in the lungs of mice

[0038] (1) Animal selection and treatment: 6- to 8-week-old female BALB / c mice were used. After obtaining the approval of the animal ethics review, the mice were kept in the Level 2 laboratory of the Animal Biosafety Laboratory. Mice were euthanized using the cervical dislocation method following the inhalation anesthetic isoflurane. The mouse lungs and salivary glands were aseptically dissected, and the removed lungs and salivary glands were immediately frozen in liquid nitrogen and then stored in a -80°C freezer.

[0039] (2) Esophageal gavage drug: select 6 to 8-week-old female BALB / c mice, and continuously gavage the drug prednisone through the esophagus for 7 days. The dose of prednisone is 1 mg / kg, and the oral drug prednisone is Glucocorticoids.

[0040] 2. C...

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Abstract

The invention discloses a method for constructing a latent infection model of cytomegalovirus in the lungs of mice. First, the mice are continuously fed with prednisone through the esophagus, and then MCMV virus is injected into the abdominal cavity of the mice. Detection technology to identify pulmonary cytomegalovirus latent infection status. The invention induces latent infection of cytomegalovirus by oral administration of prednisone to mice and intraperitoneal injection of MCMV virus, and latent infection of cytomegalovirus occurs in the lungs of mice, and a latent infection of cytomegalovirus can be constructed. Animal model, the mouse model shows the typical features of latent cytomegalovirus infection in the lungs: the expression of related proteins of the virus appears in the lung tissue. The model can be applied to scientific research in the field of cytomegalovirus infection, and lays the foundation for experimental animals to explore the pathogenic mechanism of cytomegalovirus infection.

Description

technical field [0001] The invention relates to the technical field of bioengineering, and more particularly, to a method for constructing a latent infection model of cytomegalovirus in the lungs of mice. Background technique [0002] The infection rate of Human Cytomegalovirus (HCMV) in the population reaches 60-100%, and the infection rate of HCMV among newborns in my country is as high as 96.2%. The infection rate is related to factors such as health conditions, economic conditions and geographical conditions. . HCMV is a kind of herpes virus, which has the characteristics of herpes virus infection, that is, once the human body is infected with the virus, it will carry it for life, which can lead to congenital nervous system dysplasia, induce transplant organ rejection, weaken immune defense function and other adverse outcomes, which seriously threatens life and health. , but there are currently few treatment and preventive measures. [0003] Cytomegalovirus (CMV) has ob...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/02
CPCA01K67/02A01K2267/03A01K2227/105
Inventor 张志辉孙艺凝
Owner 张志辉
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