Novel preserving fluid for microbial nucleic acid in sputum

Abstract

The invention discloses a novel preserving fluid for microbial nucleic acid in sputum. The preserving fluid comprises the following components in percentage by mass: 15-30% of nuclease inhibitor, 1.5-5% of a pH value stabilizer; 0.8%-1.5% of a chelating agent; 0.05%-0.15% of a mucus dissolving agent; 0.1 to 0.5 percent of sodium dodecyl sarcosinate; 10 to 20 percent of isopropanol; 2 to 15 percentof glycerol; the balance of sterilized water. The novel preserving fluid for microbial nucleic acid in sputum can rapidly dissolve and stabilize sputum samples, effectively inhibit nuclease activityand prevent cell lysis, has good sample antibacterial property, long preservation time, relatively simple components, capability of keeping nucleic acid at room temperature, and suitableness for wideclinical application.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a novel preservation solution for microbial nucleic acids in sputum. Background technique [0002] Phlegm is a liquid secreted by stimulation of the respiratory tract, also called sputum, and its components include mucus, foreign bodies, pathogenic microorganisms, various inflammatory cells, necrotic and exfoliated mucosal epithelial cells, etc. Currently, sputum samples are mainly used for sputum cytology detection and respiratory pathogen detection in clinical practice. Among them, identifying the type of pathogen by detecting the nucleic acid of the pathogen in the sputum sample is currently the most accurate and rapid diagnostic method for respiratory pathogens. However, in practice, nucleic acids (especially RNA) in sputum samples are extremely unstable and are usually degraded within hours under greenhouse conditions. External physical factors that cause nucleic ...

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Problems solved by technology

[0003] At present, some patents have disclosed methods for protecting nucleic acids. However, the technologies involved in the existing patents are not practical for sputum samples, and most of them still have the following problems: the existing preservation solutions are difficult to simultaneously inhibit nucleases activity and prevent cell lysis; the antibacterial effect is poor, microorganisms are easy to breed; the storage time is not long enough, etc.

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