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Application of bacillus siamensis B11 in prevention and/or treatment of Fargesia fungosa with sphaeropsis blight

A Bacillus, Mianzhu technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of human and animal poisoning, environmental pollution, etc., and achieve the effects of low production cost, long storage time and good effect.

Inactive Publication Date: 2020-11-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of chemical pesticides will pollute the environment, easily cause poisoning of humans and animals, and cause certain pests to develop resistance to pesticides to varying degrees.

Method used

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  • Application of bacillus siamensis B11 in prevention and/or treatment of Fargesia fungosa with sphaeropsis blight
  • Application of bacillus siamensis B11 in prevention and/or treatment of Fargesia fungosa with sphaeropsis blight
  • Application of bacillus siamensis B11 in prevention and/or treatment of Fargesia fungosa with sphaeropsis blight

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation and identification of Bacillus siamese B11

[0016] 1. Isolation of Bacillus siamese B11

[0017] The Bacillus siamensis (Bacillus siamensis) B11 strain of the present invention was isolated on June 15, 2017 in the rhizosphere soil of the bamboo cultivation area in Yibin, Sichuan by the plate dilution coating method, specifically the soil samples taken back were diluted with sterile water , diluted 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 A total of 5 gradients, draw 100μL of 10 -3 , 10 -4 , 10 -5 These three gradient soil dilutions were coated on NA plates (3g of beef extract, 10g of peptone, 5g of sodium chloride, 15-20g of agar powder, 1000mL of distilled water, pH7.0, mixed and packed, and autoclaved at 121°C for 30min. ), each treatment was repeated three times, cultured at 27°C for 48 hours, and single colonies with obvious differences in colony morphology were picked to enter the primary screening.

[0018] According to the differences i...

Embodiment 2

[0030] The preparation of embodiment 2 bacillus siamese B11 nanometer microemulsion

[0031] The Bacillus siamese B11 is fermented, mixed with an emulsifier, homogenized, and made into a nano-microemulsion, which specifically includes the following steps:

[0032] (1) Fermentation: Inoculate the slant seeds of Bacillus siamese into the liquid medium, the initial inoculum size is 4%, then shake and cultivate for 48 hours at 25°C, 185r / min, and 200L / h ventilation;

[0033] Wherein, the liquid medium contains the following components by weight percentage: 0.5% nano silicon dioxide, 2% sucrose, 1% tryptone, 0.1% sodium chloride and 0.05% magnesium sulfate, and the rest are sterile water, liquid medium The pH value is 7.2.

[0034] (2) Prepare nano-microemulsion: add emulsifier, stabilizer, antifreeze in the above-mentioned fermented liquid to make the percentage by weight that emulsifier, stabilizer, antifreeze account for are respectively 10%, 2%, 5% in the high-pressure mixing ...

Embodiment 3

[0036] Example 3 The prevention and treatment experiment of Mianzhu die-back disease

[0037] Preparation of Bacillus siamese B11 nano-microemulsion dilution: get 1 g of Bacillus siamese B11 nano-microemulsion prepared in Example 2, and dilute it with sterile water so that the content of live spores is 1×10 9 cfu / ml dilution.

[0038] Preparation of pathogenic bacteria liquid: 14 days after pathogenic bacteria were inoculated in PDA medium, spore suspension was prepared. Rinse a petri dish with 50ml of sterile water, suck it out with a pipette gun, filter it with absorbent cotton, filter it with a centrifuge, dilute it with sterile water, observe and calculate the spore concentration on a hemocytometer, and dilute to 1×10 6 cfu / ml. The pathogenic bacteria liquid is prepared by mixing the spore suspensions of Arthrinium paraphaeospermum and Arthrobotryum rickii in a ratio of 1:1 (by weight).

[0039] Pot control experiment: select healthy Mianzhu in Yibin Mianzhu planting ar...

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Abstract

The invention discloses an application of bacillus siamensis B11 in prevention and / or treatment ofFargesia fungosa with sphaeropsis blight. According to the invention, the bacillus siamensis B11 is used for preventing and / or treating the Fargesia fungosa with sphaeropsis blight, the bacillus siamensis B11 is very good in effect, is long in storage time and high in strain survival rate after beingprepared into the nano microemulsion, has a good prevention effect on the Fargesia fungosa with sphaeropsis blight, has good stress resistance, high temperature resistance, low temperature resistanceand drying resistance, is suitable for commercialized production, is pollution-free, green and environment-friendly, can continuously control diseases and is low in production cost.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to an application of Bacillus siamese B11 in preventing and / or treating Mianzhu blight. Background technique [0002] Mianzhu (scientific name: Bambusa intermedia Hsueh et Yi) is a Poaceae, Bougainvillea genus of arbor-like bamboo plants, distributed in the central and southern parts of Yunnan, China, widely cultivated, also found in Sichuan and Guizhou parts, the fiber degree and toughness of Mianzhu are basically Tongci bamboo, which is put into production early and has high yield, is suitable for bamboo weaving, papermaking and eating as bamboo shoots. Mianzhu die-back disease caused by Arthriniumparaphaeospermum and Arthrobotryum rickii first appears tongue-shaped or spindle-shaped lesions at the joints and forks of the main branches or branches, which are light brown at first and then purple-brown. When the lesion surrounds the branch or trunk, the upper leaves turn...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01G7/06A01G13/00A01G17/00C12R1/07
CPCA01G7/06A01G13/00A01G17/005A01N63/22C12N1/20C12N1/205C12R2001/07
Inventor 朱天辉王诗玮方馨玫曾艳玲李姝江韩珊谯天敏
Owner SICHUAN AGRI UNIV
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