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Fibrocartilage glycosaminoglycan and extraction method thereof

A technology of fibrocartilage and glycosaminoglycan, applied in the field of glycosaminoglycan extraction, can solve the problems of adding chemical reagents and high cost, and achieve the effect of low cost, high cost and simple and easy-to-operate extraction method

Active Publication Date: 2020-11-13
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a glycosaminoglycan of fibrocartilage and an extraction method thereof. The glycosaminoglycan is extracted from the fibrocartilage through a simple and easy method, which is low in cost and does not use chemical reagents, effectively It solves the problems of needing to add chemical reagents and high cost in the prior art

Method used

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  • Fibrocartilage glycosaminoglycan and extraction method thereof

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Embodiment 1

[0020] A kind of fibrocartilage glycosaminoglycan, its extraction method comprises the following steps:

[0021] (1) Take a fresh oxtail vertebra fibrous ring to remove the surrounding fat and muscle tissue, wash it, and perform 5 freeze-thaw cycles in the temperature range of -20-37°C (each freeze-thaw cycle is 24h, respectively, -20°C lasts 12h, 37°C ℃ for 12h), then cut the fibrocartilage into 3-5mm tissue pieces;

[0022] (2) Soak the tissue pieces obtained in step (1) in ultrapure water, stir at 3°C ​​for 36 hours, centrifuge to collect the supernatant, and then freeze-dry (-70°C) to obtain fibrocartilage glycosaminoglycan.

Embodiment 2

[0024] A kind of fibrocartilage glycosaminoglycan, its extraction method comprises the following steps:

[0025] (1) Take the fresh oxtail nucleus pulposus to remove the surrounding fat and muscle tissue, wash it, and carry out 5 freeze-thaw cycles in the temperature range of -20-37°C (each freeze-thaw cycle is 24h, respectively, -20°C lasts 12h, 37°C ℃ for 12h), then cut the fibrocartilage into 3-5mm tissue pieces;

[0026] (2) Soak the tissue pieces obtained in step (1) in ultrapure water, stir at 4°C for 48 hours, centrifuge to collect the supernatant, and then freeze-dry (-70°C) to obtain fibrocartilage glycosaminoglycan.

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Abstract

The invention provides fibrocartilage glycosaminoglycan and an extraction method thereof. The extraction method comprises the following steps that fresh animal fibrocartilage is taken, surrounding adipose muscle tissue is removed, cleaning is conducted, 4-6 freezing and thawing cycles are conducted in the temperature interval of-20 DEG C to 37 DEG C, and then the fibrocartilage is cut into tissueblocks with the size being 3-5 mm; the tissue blocks are soaked in ultrapure water, stirring is performed for 36-60 hours at the temperature of 3-5 DEG C, centrifuging is performed, the supernatant iscollected, and then freeze-drying is performed to obtain the cellochondral glycosaminoglycan. The invention also discloses the fibrocartilage glycosaminoglycan prepared by the extraction method. According to the method, the glycosaminoglycan is extracted from the fibrous cartilage through a simple and feasible method, the cost is low, no chemical reagent is adopted, and the problems that in the prior art, chemical reagents need to be added, and the manufacturing cost is high are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of glycosaminoglycan extraction, and in particular relates to a glycosaminoglycan of fibrocartilage and an extraction method thereof. Background technique [0002] Acidic mucopolysaccharide, also known as glycosaminoglycan (GAG, glycosaminoglycan), is a long-chain complex sugar molecule composed of disaccharide units composed of hexuronic acid, hexosamine sugar or neutral sugar connected to each other, which can be connected with proteins to form Proteoglycan (proteoglycan). Glycosaminoglycans play an important role in maintaining the elasticity of human skin and connective tissue, and maintaining the stability of the extracellular matrix of cartilage. In previous scientific studies, chemical reagents such as guanidine hydrochloride were used to extract glycosaminoglycans from fibrocartilage, and dialysis was used to remove residual chemical reagents. However, this method has a cumbersome process and compl...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0069
Inventor 姜楠应彬彬祝颂松毕瑞野王旻陈浩哲
Owner SICHUAN UNIV
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