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Method for determining content of index components in fingered citron preparation

A method of determination, the technology of bergamot, is applied in the field of determination of the content of index components in bergamot preparations, which can solve the problems of single quality judgment standard of bergamot preparations and the inability to fundamentally guarantee the efficacy of preparations, and achieve high precision, good repeatability, The effect of accurate test results

Inactive Publication Date: 2020-11-10
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to: aim at the technical problem that only tests the content of hesperidin to evaluate and control the quality of bergamot preparations in the prior art, which leads to a single quality judgment standard for bergamot preparations and cannot fundamentally guarantee the efficacy of the preparations, and provides a A method for determining the content of index components in bergamot preparations. The method simultaneously detects three index components in bergamot preparations, diosmin, hesperidin, and 5,7-dimethoxycoumarin. It is accurate and efficient, and can be used for bergamot preparations For further quality control, this method has the advantages of accurate detection results, high precision, and good repeatability. Compared with existing assay methods, it can better reflect the content of active ingredients in bergamot and related preparations

Method used

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  • Method for determining content of index components in fingered citron preparation
  • Method for determining content of index components in fingered citron preparation
  • Method for determining content of index components in fingered citron preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Content Determination of Bergamot Medicinal Materials

[0067] 1 Instruments and reagents

[0068] 1.1 Instrument

[0069] Thermo Ultimate 3000 high performance liquid chromatograph, including LPG-3400SDN pump, WPS-3000SL sampler, TCC-3000SD column oven, DAD-3000 detector and Chromeleon 7 chromatographic processing software) [Thermo Fisher Shier Technology (China) Co., Ltd.]; Discovery DV215CD 1 / 100,000 balance (Ohaus Corporation, the United States); BS124S 1 / 10,000 electronic analytical balance (Sartorius Scientific Instrument Co., Ltd.); KQ3200E ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0070] 1.2 Reagent

[0071] The reference substances diosmin (lot number PCL-#-Di130), hesperidin (lot number PCL-#-H633), and 5,7-dimethoxycoumarin (lot number PCL-#-Dn023) were purchased from Pure Chem Land company, the purity>98%.

[0072] Methanol (analytically pure, Chengdu Kelong Chemical Co., Ltd.), acetonitrile (chromatographically pure, Fisher, USA),...

Embodiment 2

[0124] Content Test of Chuanberga Granules (Alcohol Extraction)

[0125] 1 Instruments and reagents

[0126] BT25S type 1 / 100,000 electronic analytical balance (Sartorius Scientific Instrument Co., Ltd.), all the other instruments and reagents are the same as in Example 1.

[0127] 1.3 Sample

[0128] Preparation of Chuanbergam Granules (Alcohol Extraction)

[0129] (1) Fragmentation of Chuan bergamot decoction pieces, the particle diameter is about 0.2cm;

[0130] (2) add 12 times the amount of 70% ethanol to the broken pieces of bergamot decoction, extract 3 times, each 1.5h, when extracting for the first time, add 0.6 times more 70% ethanol to supplement the amount of alcohol absorbed; the extracts are filtered and combined, The filtrate was concentrated under reduced pressure into a clear paste with a relative density of 1.18 at 60°C for subsequent use;

[0131] (3) Add the mixed auxiliary material dextrin-sucrose (8:1) to the clear cream obtained in step (2), and make...

Embodiment 3

[0173] Content Determination of Chuanberga Granules (Water Extraction)

[0174] 1 Instruments and reagents

[0175] BT25S type 1 / 100,000 electronic analytical balance (Sartorius Scientific Instrument Co., Ltd.), all the other instruments and reagents are the same as in Example 1.

[0176] 1.3 Sample

[0177] Preparation of Chuanbergam Granules (Water Extraction)

[0178] (1) Fragmentation of Chuan bergamot decoction pieces, the particle diameter is about 0.2cm;

[0179] (2) Add 12 times the amount of water to the crushed Chuan bergamot decoction pieces, extract 3 times, each 2h, when extracting for the first time, add 3.5 times the amount of water to make up for the water absorption; the extracts are filtered and merged, and the filtrate is concentrated under reduced pressure to 60 Clear ointment with a relative density of 1.15 to 1.18 at ℃, set aside;

[0180](3) Add the mixed auxiliary material dextrin-sucrose (8:1) to the clear cream obtained in step (2), and make a sof...

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Abstract

The invention relates to a method for determining the content of index components in a fingered citron preparation. The method comprises the following steps: step 1, preparing a mixed reference solution and a test solution; 2, carrying out high performance liquid chromatography detection; 3, establishing three index component standard curves; and 4, determining the content of three index components of geraniin, hesperidin and 5, 7dimethoxycoumarin in the test solution. The method simultaneously detects three index components of geraniin, hesperidin and 5, 7dimethoxycoumarin in the fingered citron preparation, is accurate and efficient, can be used for further quality control of the fingered citron preparation, has the advantages of accurate detection result, high precision and good repeatability, Compared with the existing determination method, the method can better reflect the content of effective components of fingered citron and related preparations.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicines, and in particular relates to a method for measuring the contents of index components in bergamot preparations. Background technique [0002] Bergamot is the dried fruit of Rutaceae citrus plant Bergamot. It has the effects of soothing the liver and regulating qi, harmonizing the stomach and relieving pain, drying dampness and resolving phlegm. For stagnation of liver and stomach qi, distending pain in the chest and hypochondriac, fullness in the epigastric cavity, lack of appetite, vomiting, cough with excessive phlegm. Bergamot can be made into various Chinese medicinal preparations, and taking it for a long time has the effect of health care and longevity. [0003] However, in the quality standards of bergamot preparations on the market, only some preparations have carried out the content determination of hesperidin, and the content of hesperidin in be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679
Inventor 邓放胡瀚文彭腾郑振兴杨天龙
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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