Combined marker for detecting relationship between antidepressant dose-effect and dose-toxicity of Chinese thorowax root petroleum ether part and application of combined marker

Abstract

The invention belongs to the technical field of application of endogenous small-molecule metabolites of biological samples, and provides a combined marker for detecting an anti-depression amount-effect and amount-toxicity relationship of a radix bupleuri petroleum ether part and application of the combined marker. The amount-effect marker comprises L-isoleucine / leucine, L-carnitine, L-acetylcarnitine, adenine and 2-phenylacetamide; the quantity-toxicity marker is prepared from L-carnitine, valine, hydroxysuccinic acid, 2'-adenosine phosphate and 2-sulfuric acid ascorbic acid. The method is used for evaluating the relationship between the anti-depression dose-effect and dose-toxicity of the petroleum ether part of bupleurum chinense, and solves the problem of dose application of the traditional Chinese medicine bupleurum chinense in evaluating the drug effect and safety of depression treatment. The method can be used for sensitively and visually evaluating the relationship between the in-vivo action and dosage of the bupleurum petroleum ether part, and providing technical support for the drug effect and toxic dosage of the traditional Chinese medicine bupleurum, and a new method isprovided for exploring the effectiveness and safety of the traditional Chinese medicine bupleurum.

Description

technical field [0001] The invention belongs to the technical field of application of endogenous small molecule metabolites of biological samples, and specifically relates to a combination marker for detecting the antidepressant dose-effect and dose-toxicity relationship of Bupleurum petroleum ether and its application. Use liquid chromatography-mass spectrometry to detect endogenous small molecule compounds in biological samples, use multivariate data processing methods to screen compounds, and combine mathematical models to obtain dose-effect and dose-toxicity indices, which are used to evaluate the dose-drug / toxicity relationship. Background technique [0002] The dose-efficiency / toxicity relationship of traditional Chinese medicine is the essence of clinical medicine in traditional Chinese medicine. For a long time, due to the multi-component and multi-target mode of action of traditional Chinese medicine, how to comprehensively evaluate the dose-effect / toxicity relations...

AI Technical Summary

Problems solved by technology

[0004] At the same time, there is still a lack of objective quantitative indicators in the existing evaluation standards, especially subjective human evaluation or qualitative evaluation in the process of drug efficacy evaluation, such as tissue sections, imaging techniques, various electron microscope examinations, etc.

In addition, although metabolomics technology has been used for drug efficacy evaluation, the amount of metabolomics data is huge, and it is difficult to apply a focused evaluation model in clinical practice. In short, the existing methods of dose-effect-toxicity evaluation have limitations. Larger, lack of quantitative standards, lack of objectivity, comprehensiveness, pertinence and innovation, which is not conducive to the objective and accurate evaluation of the dose-effect / toxicity of traditional Chinese medicine, and has become a huge obstacle that seriously restricts pharmacological research and a major problem that plagues pharmacological research. problem

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