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Endophytic fungus and application thereof in dye pollutant treatment

A technology for pollutant treatment and endophytic fungi, applied in biological water/sewage treatment, water pollutants, microbial-based methods, etc., can solve problems such as difficult recycling, narrow degradation spectrum, and application limitations

Active Publication Date: 2020-10-30
ANHUI UNIVERSITY OF TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these dye-degrading strains that have been reported so far generally have problems such as narrow degradation spectrum and difficulty in recycling, which limit their application.

Method used

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  • Endophytic fungus and application thereof in dye pollutant treatment
  • Endophytic fungus and application thereof in dye pollutant treatment
  • Endophytic fungus and application thereof in dye pollutant treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: Isolation and screening of endophytic fungi

[0020] Tissue imprinting method was used for separation and screening. The separation and screening method was as follows: take an appropriate amount of fresh blueberries, wash them, sterilize them in 75% ethanol on an ultra-clean workbench for 1 min, soak them in sterile water for 20 seconds, and then place them in 10% ethanol. Disinfect in sodium hypochlorite solution for 3 minutes, rinse with sterile water for 5 times, and apply the sterile water of the last rinse to the TWA solid separation medium to test whether the disinfection is thorough. Use sterile filter paper to blot the remaining liquid on the blueberries, then cut each blueberry into 3-4 pieces, and blot them on the TWA solid separation medium, and blot 5 pieces for each petri dish containing the TWA solid separation medium. The formula of TWA solid separation medium is 8g agar powder and 1000mL deionized water, and autoclaved at 121°C for 15min.

...

Embodiment 2

[0030] Embodiment 2: the application of endophytic fungus in dye pollutant treatment

[0031] Preparation method of liquid medium for endophytic fungi: Prepare PDB liquid medium containing 500mg / L concentration of neutral red, Congo red, amide black 10B, magenta, methyl red, naphthol green B and cadmium black T dyes respectively: Wash and peel the potatoes, weigh 100g and cut into slices, add 500mL of water, boil for about 30min, filter it into a 500mL Erlenmeyer flask with gauze, add 10g of glucose and 10g of agar, stir with a glass rod, and make up after cooling down Water to 500mL, divided into 100mL Erlenmeyer flasks, each Erlenmeyer flask is 30mL quantitatively, sealed with a rubber stopper, sterilized under high pressure at 121°C for 20 minutes, after the sterilization is completed, take out the Erlenmeyer flasks and transfer them to a clean bench when the temperature drops to 50°C After the temperature dropped to room temperature, take 1.5 mL of neutral red, Congo red, ...

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Abstract

The invention provides endophytic fungus and an application of the endophytic fungus in dye pollutant treatment. The endophytic fungus is a pure culture strain separated from blueberry fruits by adopting a tissue imprinting method, the penicillium janthinellum LM5 gene is determined to be penicillium janthinellum through molecular biological identification, the penicillium janthinellum LM5 gene isnamed as penicillium janthinellum LM5, the strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2019965. The endophytic fungus disclosed by theinvention can be used for treating dye pollutants and has the characteristics of good adsorption effect, various degradation substrates, simplicity in operation, easiness in solid-liquid separation, low cost, environmental friendliness and the like.

Description

technical field [0001] The invention relates to the technical field of endophytic fungi, in particular to an endophytic fungus and its application in the treatment of dye pollutants. Background technique [0002] The resource and environmental constraints accumulated in the long-term rapid development have become increasingly prominent, and freshwater pollution caused by dyes has become one of the prominent environmental problems. These pollutants not only pollute the water body, exacerbate the situation of water shortage in my country, but also seriously damage the ecological balance and endanger human health due to their biological toxicity and three effects (teratogenic, carcinogenic and mutagenic). According to statistics, there are more than 100,000 types of dyes on the market, and the annual output of dyes exceeds 700,000 tons, of which more than 10% of the dyes are directly discharged into rivers and lakes during production and use, causing water pollution from the su...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C12R1/80C02F101/30C02F103/30
CPCC02F3/347C02F2101/308C02F2103/30C12N1/145C12R2001/80
Inventor 韩新亚郑德严菊芬张妍金奇邵晴晴汪莹
Owner ANHUI UNIVERSITY OF TECHNOLOGY
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